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A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus.
Bull Entomol Res. 2009 Dec; 99(6):573-82.BE

Abstract

A real-time PCR assay based on TaqMan technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex.

Authors+Show Affiliations

Agricultural Research Institute, 1516 Nicosia, PO Box 22016 Cyprus. l.papayiannis@arinet.ari.gov.cyNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19203404

Citation

Papayiannis, L C., et al. "A Real-time PCR Assay to Differentiate the B and Q Biotypes of the Bemisia Tabaci Complex in Cyprus." Bulletin of Entomological Research, vol. 99, no. 6, 2009, pp. 573-82.
Papayiannis LC, Brown JK, Seraphides NA, et al. A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus. Bull Entomol Res. 2009;99(6):573-82.
Papayiannis, L. C., Brown, J. K., Seraphides, N. A., Hadjistylli, M., Ioannou, N., & Katis, N. I. (2009). A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus. Bulletin of Entomological Research, 99(6), 573-82. https://doi.org/10.1017/S0007485308006603
Papayiannis LC, et al. A Real-time PCR Assay to Differentiate the B and Q Biotypes of the Bemisia Tabaci Complex in Cyprus. Bull Entomol Res. 2009;99(6):573-82. PubMed PMID: 19203404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A real-time PCR assay to differentiate the B and Q biotypes of the Bemisia tabaci complex in Cyprus. AU - Papayiannis,L C, AU - Brown,J K, AU - Seraphides,N A, AU - Hadjistylli,M, AU - Ioannou,N, AU - Katis,N I, Y1 - 2009/02/10/ PY - 2009/2/11/entrez PY - 2009/2/11/pubmed PY - 2010/2/2/medline SP - 573 EP - 82 JF - Bulletin of entomological research JO - Bull Entomol Res VL - 99 IS - 6 N2 - A real-time PCR assay based on TaqMan technology was developed and evaluated for the rapid detection of the B and Q biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). A survey was conducted during 2005-2007 in order to identify the distribution and prevalence of B. tabaci biotypes in Cyprus using the real-time PCR assay. More than 700 adult whiteflies collected from 35 cultivated and weed plant species were individually haplotyped using TaqMan PCR, and the results of the assay were validated by restriction fragment length polymorphism analysis and DNA sequencing of the mitochondrial cytochrome oxidase I (mtCOI) gene. Two biotypes, B and Q, were identified in the collected plant species on the island. The real-time PCR and RFLP assay consistently yielded the same results, although the real-time assay was more sensitive and less time consuming. Phylogenetic analysis of the mtCOI DNA sequences corroborated the identity of the B and Q biotypes 100% of the time and by phylogenetic analysis the haplotypes grouped, as expected, in the major North African-Mediterranean-Middle Eastern clade of the B. tabaci complex. SN - 1475-2670 UR - https://www.unboundmedicine.com/medline/citation/19203404/A_real_time_PCR_assay_to_differentiate_the_B_and_Q_biotypes_of_the_Bemisia_tabaci_complex_in_Cyprus_ L2 - https://www.cambridge.org/core/product/identifier/S0007485308006603/type/journal_article DB - PRIME DP - Unbound Medicine ER -