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Molecularly imprinted solid-phase extraction for the selective determination of 17beta-estradiol in fishery samples with high performance liquid chromatography.
Talanta. 2009 Apr 30; 78(2):442-7.T

Abstract

A molecularly imprinted polymer (MIP) has been synthesized by a thermo-polymerization method using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, acetonitrile as porogenic solvent, and 17beta-estradiol as template. The MIP showed obvious affinity for 17beta-estradiol in acetonitrile solution, which was confirmed by absorption experiments. After optimization of molecularly imprinted solid-phase extraction (MISPE) conditions, three structurally related estrogenic compounds (17beta-estradiol, estriol, and diethylstilbestrol) were used to evaluate the selectivity of the MIP cartridges. The MIP cartridges exhibited highly selectivity for E(2), the recoveries were 84.8+/-6.53% for MIPs and 19.1+/-1.93% for non-imprinted polymer (NIP) cartridges. The detection and quantification limits correspond to 0.023 and 0.076 mg L(-1). Furthermore, the MISPE methods were used to selectively extract E(2) from fish and prawn tissue prior to HPLC analysis. This MISPE-HPLC procedure could eliminate all matrix interference simultaneously and had good recoveries (78.3-84.5%).

Authors+Show Affiliations

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19203606

Citation

Jiang, Tianhe, et al. "Molecularly Imprinted Solid-phase Extraction for the Selective Determination of 17beta-estradiol in Fishery Samples With High Performance Liquid Chromatography." Talanta, vol. 78, no. 2, 2009, pp. 442-7.
Jiang T, Zhao L, Chu B, et al. Molecularly imprinted solid-phase extraction for the selective determination of 17beta-estradiol in fishery samples with high performance liquid chromatography. Talanta. 2009;78(2):442-7.
Jiang, T., Zhao, L., Chu, B., Feng, Q., Yan, W., & Lin, J. M. (2009). Molecularly imprinted solid-phase extraction for the selective determination of 17beta-estradiol in fishery samples with high performance liquid chromatography. Talanta, 78(2), 442-7. https://doi.org/10.1016/j.talanta.2008.11.047
Jiang T, et al. Molecularly Imprinted Solid-phase Extraction for the Selective Determination of 17beta-estradiol in Fishery Samples With High Performance Liquid Chromatography. Talanta. 2009 Apr 30;78(2):442-7. PubMed PMID: 19203606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid-phase extraction for the selective determination of 17beta-estradiol in fishery samples with high performance liquid chromatography. AU - Jiang,Tianhe, AU - Zhao,Lixia, AU - Chu,Baolin, AU - Feng,Qinzhong, AU - Yan,Wei, AU - Lin,Jin-Ming, Y1 - 2008/12/09/ PY - 2008/09/10/received PY - 2008/11/24/revised PY - 2008/11/24/accepted PY - 2009/2/11/entrez PY - 2009/2/11/pubmed PY - 2009/4/14/medline SP - 442 EP - 7 JF - Talanta JO - Talanta VL - 78 IS - 2 N2 - A molecularly imprinted polymer (MIP) has been synthesized by a thermo-polymerization method using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, acetonitrile as porogenic solvent, and 17beta-estradiol as template. The MIP showed obvious affinity for 17beta-estradiol in acetonitrile solution, which was confirmed by absorption experiments. After optimization of molecularly imprinted solid-phase extraction (MISPE) conditions, three structurally related estrogenic compounds (17beta-estradiol, estriol, and diethylstilbestrol) were used to evaluate the selectivity of the MIP cartridges. The MIP cartridges exhibited highly selectivity for E(2), the recoveries were 84.8+/-6.53% for MIPs and 19.1+/-1.93% for non-imprinted polymer (NIP) cartridges. The detection and quantification limits correspond to 0.023 and 0.076 mg L(-1). Furthermore, the MISPE methods were used to selectively extract E(2) from fish and prawn tissue prior to HPLC analysis. This MISPE-HPLC procedure could eliminate all matrix interference simultaneously and had good recoveries (78.3-84.5%). SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19203606/Molecularly_imprinted_solid_phase_extraction_for_the_selective_determination_of_17beta_estradiol_in_fishery_samples_with_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(08)00857-6 DB - PRIME DP - Unbound Medicine ER -