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The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever.
Trans R Soc Trop Med Hyg. 2009 Apr; 103(4):413-9.TR

Abstract

Dengue infection is a major public health problem affecting millions of people living in tropical countries. With no suitable vaccines and specific antiviral drugs, treatment for dengue is usually symptomatic and supportive. Early diagnosis and recognition of severe disease is therefore crucial for better management of the patient. Two-dimension electrophoresis was used to identify disease-associated proteins that can be used for diagnosis and as drug targets for treatment. Two markers, identified by mass spectrometry analysis as alpha1-antitrypsin and NS1 proteins were found to be upregulated in dengue fever (DF; n=10) and dengue haemorrhagic fever (DHF; n=10) patients compared with healthy individuals (n=8). Both alpha1-antitrypsin and NS1 proteins were overexpressed two-fold in DHF patients compared with DF patients. Our study suggests that alpha1-antitrypsin and NS1 protein could be used as biomarkers as early indicators of DHF risk among patients with suspected dengue infection.

Authors+Show Affiliations

Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19203772

Citation

Thayan, Ravindran, et al. "The Use of Two-dimension Electrophoresis to Identify Serum Biomarkers From Patients With Dengue Haemorrhagic Fever." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 103, no. 4, 2009, pp. 413-9.
Thayan R, Huat TL, See LL, et al. The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever. Trans R Soc Trop Med Hyg. 2009;103(4):413-9.
Thayan, R., Huat, T. L., See, L. L., Tan, C. P., Khairullah, N. S., Yusof, R., & Devi, S. (2009). The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103(4), 413-9. https://doi.org/10.1016/j.trstmh.2008.12.018
Thayan R, et al. The Use of Two-dimension Electrophoresis to Identify Serum Biomarkers From Patients With Dengue Haemorrhagic Fever. Trans R Soc Trop Med Hyg. 2009;103(4):413-9. PubMed PMID: 19203772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever. AU - Thayan,Ravindran, AU - Huat,Tan Lian, AU - See,Lucy Lum Chai, AU - Tan,Christina Phoay Lay, AU - Khairullah,Nor Shahidah, AU - Yusof,Rohana, AU - Devi,Shamala, Y1 - 2009/02/08/ PY - 2007/03/20/received PY - 2008/12/23/revised PY - 2008/12/23/accepted PY - 2009/2/11/entrez PY - 2009/2/11/pubmed PY - 2009/7/28/medline SP - 413 EP - 9 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 103 IS - 4 N2 - Dengue infection is a major public health problem affecting millions of people living in tropical countries. With no suitable vaccines and specific antiviral drugs, treatment for dengue is usually symptomatic and supportive. Early diagnosis and recognition of severe disease is therefore crucial for better management of the patient. Two-dimension electrophoresis was used to identify disease-associated proteins that can be used for diagnosis and as drug targets for treatment. Two markers, identified by mass spectrometry analysis as alpha1-antitrypsin and NS1 proteins were found to be upregulated in dengue fever (DF; n=10) and dengue haemorrhagic fever (DHF; n=10) patients compared with healthy individuals (n=8). Both alpha1-antitrypsin and NS1 proteins were overexpressed two-fold in DHF patients compared with DF patients. Our study suggests that alpha1-antitrypsin and NS1 protein could be used as biomarkers as early indicators of DHF risk among patients with suspected dengue infection. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/19203772/The_use_of_two_dimension_electrophoresis_to_identify_serum_biomarkers_from_patients_with_dengue_haemorrhagic_fever_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035-9203(08)00576-2 DB - PRIME DP - Unbound Medicine ER -