Tags

Type your tag names separated by a space and hit enter

Role of NAD(P)H oxidase in transforming growth factor-beta1-induced monocyte chemoattractant protein-1 and interleukin-6 expression in rat renal tubular epithelial cells.
Nephrology (Carlton). 2009 Apr; 14(3):302-10.N

Abstract

AIM

This study investigated the role of NAD(P)H oxidase in transforming growth factor-beta1 (TGF-beta1)-induced reactive oxygen species (ROS) generation, monocyte chemoattractant protein-1 (MCP-1), and interleukin-6 (IL-6) expression in rat renal tubular epithelial NRK-52E cells.

METHODS

The cells were treated with 10 ng/mL TGF-beta1, either in the presence or absence of the NAD(P)H oxidase inhibitor, diphenyleneiodonium (DPI), or short hairpin RNA (shRNA) suppressing p67phox expression. Expression of NAD(P)H oxidase subunits, MCP-1, and IL-6 at the mRNA levels was detected by reverse transcription polymerase chain reaction, while expression of NAD(P)H oxidase subunit p67phox protein was analyzed by western blot and MCP-1 by enzyme-linked immunosorbent assay. The cellular ROS generation was visualized using 2',7'-dichlorodihydrofluorescein diacetate by confocal microscopy.

RESULTS

Compared to control, TGF-beta1 upregulated NAD(P)H oxidase subunit p67phox mRNA by 3.59-fold (P < 0.01), but had no effect on p22phox, gp91phox and p47phox NAD(P)H subunits. TGF-beta1 was also able to significantly increase intracellular ROS (P < 0.05), MCP-1 (P < 0.01) and IL-6 (P < 0.05) expression in NRK-52E cells. Further studies showed that generation of ROS and upregulation of MCP-1 and IL-6 by TGF-beta1 were significantly blocked by addition of DPI or shRNA-p67phox (P < 0.01), suggesting that these effects were NAD(P)H oxidase-dependent.

CONCLUSION

TGF-beta1 differentially regulates the expression of NAD(P)H oxidase subunits and mediates MCP-1 and IL-6 expression in rat renal tubular cells via the NAD(P)H oxidase/p67phox-dependent mechanism.

Authors+Show Affiliations

Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19207862

Citation

Zhang, Haiyan, et al. "Role of NAD(P)H Oxidase in Transforming Growth Factor-beta1-induced Monocyte Chemoattractant Protein-1 and Interleukin-6 Expression in Rat Renal Tubular Epithelial Cells." Nephrology (Carlton, Vic.), vol. 14, no. 3, 2009, pp. 302-10.
Zhang H, Jiang Z, Chang J, et al. Role of NAD(P)H oxidase in transforming growth factor-beta1-induced monocyte chemoattractant protein-1 and interleukin-6 expression in rat renal tubular epithelial cells. Nephrology (Carlton). 2009;14(3):302-10.
Zhang, H., Jiang, Z., Chang, J., Li, X., Zhu, H., Lan, H. Y., Zhou, S. F., & Yu, X. (2009). Role of NAD(P)H oxidase in transforming growth factor-beta1-induced monocyte chemoattractant protein-1 and interleukin-6 expression in rat renal tubular epithelial cells. Nephrology (Carlton, Vic.), 14(3), 302-10. https://doi.org/10.1111/j.1440-1797.2008.01072.x
Zhang H, et al. Role of NAD(P)H Oxidase in Transforming Growth Factor-beta1-induced Monocyte Chemoattractant Protein-1 and Interleukin-6 Expression in Rat Renal Tubular Epithelial Cells. Nephrology (Carlton). 2009;14(3):302-10. PubMed PMID: 19207862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of NAD(P)H oxidase in transforming growth factor-beta1-induced monocyte chemoattractant protein-1 and interleukin-6 expression in rat renal tubular epithelial cells. AU - Zhang,Haiyan, AU - Jiang,Zongpei, AU - Chang,Jie, AU - Li,Xiaoyan, AU - Zhu,Hengmei, AU - Lan,Hui Y, AU - Zhou,Shu-Feng, AU - Yu,Xueqing, Y1 - 2009/02/03/ PY - 2009/2/12/entrez PY - 2009/2/12/pubmed PY - 2009/7/16/medline SP - 302 EP - 10 JF - Nephrology (Carlton, Vic.) JO - Nephrology (Carlton) VL - 14 IS - 3 N2 - AIM: This study investigated the role of NAD(P)H oxidase in transforming growth factor-beta1 (TGF-beta1)-induced reactive oxygen species (ROS) generation, monocyte chemoattractant protein-1 (MCP-1), and interleukin-6 (IL-6) expression in rat renal tubular epithelial NRK-52E cells. METHODS: The cells were treated with 10 ng/mL TGF-beta1, either in the presence or absence of the NAD(P)H oxidase inhibitor, diphenyleneiodonium (DPI), or short hairpin RNA (shRNA) suppressing p67phox expression. Expression of NAD(P)H oxidase subunits, MCP-1, and IL-6 at the mRNA levels was detected by reverse transcription polymerase chain reaction, while expression of NAD(P)H oxidase subunit p67phox protein was analyzed by western blot and MCP-1 by enzyme-linked immunosorbent assay. The cellular ROS generation was visualized using 2',7'-dichlorodihydrofluorescein diacetate by confocal microscopy. RESULTS: Compared to control, TGF-beta1 upregulated NAD(P)H oxidase subunit p67phox mRNA by 3.59-fold (P < 0.01), but had no effect on p22phox, gp91phox and p47phox NAD(P)H subunits. TGF-beta1 was also able to significantly increase intracellular ROS (P < 0.05), MCP-1 (P < 0.01) and IL-6 (P < 0.05) expression in NRK-52E cells. Further studies showed that generation of ROS and upregulation of MCP-1 and IL-6 by TGF-beta1 were significantly blocked by addition of DPI or shRNA-p67phox (P < 0.01), suggesting that these effects were NAD(P)H oxidase-dependent. CONCLUSION: TGF-beta1 differentially regulates the expression of NAD(P)H oxidase subunits and mediates MCP-1 and IL-6 expression in rat renal tubular cells via the NAD(P)H oxidase/p67phox-dependent mechanism. SN - 1440-1797 UR - https://www.unboundmedicine.com/medline/citation/19207862/Role_of_NAD_P_H_oxidase_in_transforming_growth_factor_beta1_induced_monocyte_chemoattractant_protein_1_and_interleukin_6_expression_in_rat_renal_tubular_epithelial_cells_ L2 - https://doi.org/10.1111/j.1440-1797.2008.01072.x DB - PRIME DP - Unbound Medicine ER -