Tags

Type your tag names separated by a space and hit enter

Smed-Evi/Wntless is required for beta-catenin-dependent and -independent processes during planarian regeneration.
Development 2009; 136(6):905-10D

Abstract

Planarians can regenerate a whole animal from only a small piece of their body, and have become an important model for stem cell biology. To identify regenerative processes dependent on Wnt growth factors in the planarian Schmidtea mediterranea (Smed), we analyzed RNAi phenotypes of Evi, a transmembrane protein specifically required for the secretion of Wnt ligands. We show that, during regeneration, Smed-evi loss-of-function prevents posterior identity, leading to two-headed planarians that resemble Smed-beta-catenin1 RNAi animals. In addition, we observe regeneration defects of the nervous system that are not found after Smed-beta-catenin1 RNAi. By systematic knockdown of all putative Smed Wnts in regenerating planarians, we identify Smed-WntP-1 and Smed-Wnt11-2 as the putative posterior organizers, and demonstrate that Smed-Wnt5 is a regulator of neuronal organization and growth. Thus, our study provides evidence that planarian Wnts are major regulators of regeneration, and that they signal through beta-catenin-dependent and -independent pathways.

Authors+Show Affiliations

Department of Genetics and Institute of Biomedicine of the University of Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19211673

Citation

Adell, Teresa, et al. "Smed-Evi/Wntless Is Required for Beta-catenin-dependent and -independent Processes During Planarian Regeneration." Development (Cambridge, England), vol. 136, no. 6, 2009, pp. 905-10.
Adell T, Salò E, Boutros M, et al. Smed-Evi/Wntless is required for beta-catenin-dependent and -independent processes during planarian regeneration. Development. 2009;136(6):905-10.
Adell, T., Salò, E., Boutros, M., & Bartscherer, K. (2009). Smed-Evi/Wntless is required for beta-catenin-dependent and -independent processes during planarian regeneration. Development (Cambridge, England), 136(6), pp. 905-10. doi:10.1242/dev.033761.
Adell T, et al. Smed-Evi/Wntless Is Required for Beta-catenin-dependent and -independent Processes During Planarian Regeneration. Development. 2009;136(6):905-10. PubMed PMID: 19211673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Smed-Evi/Wntless is required for beta-catenin-dependent and -independent processes during planarian regeneration. AU - Adell,Teresa, AU - Salò,Emili, AU - Boutros,Michael, AU - Bartscherer,Kerstin, Y1 - 2009/02/11/ PY - 2009/2/13/entrez PY - 2009/2/13/pubmed PY - 2009/6/2/medline SP - 905 EP - 10 JF - Development (Cambridge, England) JO - Development VL - 136 IS - 6 N2 - Planarians can regenerate a whole animal from only a small piece of their body, and have become an important model for stem cell biology. To identify regenerative processes dependent on Wnt growth factors in the planarian Schmidtea mediterranea (Smed), we analyzed RNAi phenotypes of Evi, a transmembrane protein specifically required for the secretion of Wnt ligands. We show that, during regeneration, Smed-evi loss-of-function prevents posterior identity, leading to two-headed planarians that resemble Smed-beta-catenin1 RNAi animals. In addition, we observe regeneration defects of the nervous system that are not found after Smed-beta-catenin1 RNAi. By systematic knockdown of all putative Smed Wnts in regenerating planarians, we identify Smed-WntP-1 and Smed-Wnt11-2 as the putative posterior organizers, and demonstrate that Smed-Wnt5 is a regulator of neuronal organization and growth. Thus, our study provides evidence that planarian Wnts are major regulators of regeneration, and that they signal through beta-catenin-dependent and -independent pathways. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/19211673/Smed_Evi/Wntless_is_required_for_beta_catenin_dependent_and__independent_processes_during_planarian_regeneration_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=19211673 DB - PRIME DP - Unbound Medicine ER -