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Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets.
J Physiol Pharmacol 2008; 59(4):823-35JP

Abstract

The antioxidant properties of extract from berries of Aronia melanocarpa (chokeberry) containing: anthocyanidines, phenolic acids and quercetine glycosides on oxidative/nitrative stress induced by peroxynitrite (ONOO(-), a powerful physiological oxidant, nitrating species and inflammatory mediator) in human blood platelets were studied in vitro. The extract from A. melanocarpa (5 - 50 microg/mL) significantly inhibited platelet protein carbonylation (measured by ELISA method) and thiol oxidation estimated with 5,5'-dithio-bis(2-nitro-benzoic acid) (DTNB) induced by peroxynitrite (0.1 mM) (IC(50)--35 microg/mL for protein carbonylation, and IC(50)--33 microg/mL for protein thiol oxidation). The tested extract only slightly reduced platelet protein nitration (measured by C- ELISA method). The extract also caused a distinct reduction of platelet lipid peroxidation induced by peroxynitrite. Moreover, in our preliminary experiments we observed that the extract (50 microg/mL) reduced oxidative/nitrative stress in blood platelets from patients with breast cancer. The obtained results indicate that in vitro the extract from A. melanocarpa has the protective effects against peroxynitrite-induced oxidative/nitrative damage to the human platelet proteins and lipids. The extract from A. melanocarpa seems to be also useful as an antioxidant in patients with breast cancer.

Authors+Show Affiliations

Department of General Biochemistry, University of Lodz, Poland. olasb@biol.uni.lodz.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19212014

Citation

Olas, B, et al. "Studies On Antioxidant Properties of Polyphenol-rich Extract From Berries of Aronia Melanocarpa in Blood Platelets." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 59, no. 4, 2008, pp. 823-35.
Olas B, Wachowicz B, Nowak P, et al. Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets. J Physiol Pharmacol. 2008;59(4):823-35.
Olas, B., Wachowicz, B., Nowak, P., Kedzierska, M., Tomczak, A., Stochmal, A., ... Piekarski, J. (2008). Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 59(4), pp. 823-35.
Olas B, et al. Studies On Antioxidant Properties of Polyphenol-rich Extract From Berries of Aronia Melanocarpa in Blood Platelets. J Physiol Pharmacol. 2008;59(4):823-35. PubMed PMID: 19212014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets. AU - Olas,B, AU - Wachowicz,B, AU - Nowak,P, AU - Kedzierska,M, AU - Tomczak,A, AU - Stochmal,A, AU - Oleszek,W, AU - Jeziorski,A, AU - Piekarski,J, PY - 2008/02/28/received PY - 2008/06/10/accepted PY - 2009/2/13/entrez PY - 2009/2/13/pubmed PY - 2009/4/9/medline SP - 823 EP - 35 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J. Physiol. Pharmacol. VL - 59 IS - 4 N2 - The antioxidant properties of extract from berries of Aronia melanocarpa (chokeberry) containing: anthocyanidines, phenolic acids and quercetine glycosides on oxidative/nitrative stress induced by peroxynitrite (ONOO(-), a powerful physiological oxidant, nitrating species and inflammatory mediator) in human blood platelets were studied in vitro. The extract from A. melanocarpa (5 - 50 microg/mL) significantly inhibited platelet protein carbonylation (measured by ELISA method) and thiol oxidation estimated with 5,5'-dithio-bis(2-nitro-benzoic acid) (DTNB) induced by peroxynitrite (0.1 mM) (IC(50)--35 microg/mL for protein carbonylation, and IC(50)--33 microg/mL for protein thiol oxidation). The tested extract only slightly reduced platelet protein nitration (measured by C- ELISA method). The extract also caused a distinct reduction of platelet lipid peroxidation induced by peroxynitrite. Moreover, in our preliminary experiments we observed that the extract (50 microg/mL) reduced oxidative/nitrative stress in blood platelets from patients with breast cancer. The obtained results indicate that in vitro the extract from A. melanocarpa has the protective effects against peroxynitrite-induced oxidative/nitrative damage to the human platelet proteins and lipids. The extract from A. melanocarpa seems to be also useful as an antioxidant in patients with breast cancer. SN - 1899-1505 UR - https://www.unboundmedicine.com/medline/citation/19212014/Studies_on_antioxidant_properties_of_polyphenol_rich_extract_from_berries_of_Aronia_melanocarpa_in_blood_platelets_ L2 - http://www.jpp.krakow.pl/journal/archive/12_08/pdf/823_12_08_article.pdf DB - PRIME DP - Unbound Medicine ER -