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Stem cell factor-KIT signalling plays a pivotal role in regulating pigmentation in mammalian hair.
J Pathol. 2009 May; 218(1):30-9.JP

Abstract

Hair greying is one of the most distinct but least comprehended features of senescence. The signalling of stem cell factor (SCF) and its receptor KIT has been documented to regulate essential roles in the maintenance of embryonic melanocyte lineages and postnatal cutaneous melanogenesis, although little is known about its detailed mechanisms in postnatal hair pigmentation. To address this, anagen human hair follicles and C57BL/6 murine pelage were analysed in this study. Molecular biological analyses of murine follicular skin indicated a significant increase of membrane-bound SCF expression, reaching its peak 8-16 days after anagen induction in concert with the escalation of cutaneous tyrosinase activity and corresponding pigmentation. Administration of KIT-neutralizing antibody abolished MITF and tyrosinase expressions, resulting in a reversible hair depigmentation in murine regenerated hair and human hair organ culture. Quantitative RT-PCR of human hair follicles indicated that KIT expression as well as the expression of several melanogenic factors, including MITF, was significantly lower in unpigmented than in pigmented follicles. Taken together, these data revealed a pivotal role of SCF-KIT signalling in the maintenance of human hair follicle melanogenesis during the anagen cycle and its involvement in physiological ageing of the hair follicle pigmentary unit.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hachiya A
Kao Biological Science Laboratories, Haga, Tochigi, Japan.
Sriwiriyanont P
No affiliation info available
Kobayashi T
No affiliation info available
Nagasawa A
No affiliation info available
Yoshida H
No affiliation info available
Ohuchi A
No affiliation info available
Kitahara T
No affiliation info available
Visscher MO
No affiliation info available
Takema Y
No affiliation info available
Tsuboi R
No affiliation info available
Boissy RE
No affiliation info available

MeSH

AdultAgedAnimalsFemaleGene ExpressionHair ColorHair FollicleHumansImmunohistochemistryMelanocytesMiceMice, Inbred C57BLMiddle AgedProto-Oncogene Proteins c-kitRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSignal TransductionStem Cell FactorTissue Culture TechniquesYoung Adult

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19214986

Citation

Hachiya, Akira, et al. "Stem Cell factor-KIT Signalling Plays a Pivotal Role in Regulating Pigmentation in Mammalian Hair." The Journal of Pathology, vol. 218, no. 1, 2009, pp. 30-9.
Hachiya A, Sriwiriyanont P, Kobayashi T, et al. Stem cell factor-KIT signalling plays a pivotal role in regulating pigmentation in mammalian hair. J Pathol. 2009;218(1):30-9.
Hachiya, A., Sriwiriyanont, P., Kobayashi, T., Nagasawa, A., Yoshida, H., Ohuchi, A., Kitahara, T., Visscher, M. O., Takema, Y., Tsuboi, R., & Boissy, R. E. (2009). Stem cell factor-KIT signalling plays a pivotal role in regulating pigmentation in mammalian hair. The Journal of Pathology, 218(1), 30-9. https://doi.org/10.1002/path.2503
Hachiya A, et al. Stem Cell factor-KIT Signalling Plays a Pivotal Role in Regulating Pigmentation in Mammalian Hair. J Pathol. 2009;218(1):30-9. PubMed PMID: 19214986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stem cell factor-KIT signalling plays a pivotal role in regulating pigmentation in mammalian hair. AU - Hachiya,Akira, AU - Sriwiriyanont,Penkanok, AU - Kobayashi,Takeshi, AU - Nagasawa,Azumi, AU - Yoshida,Hiroshi, AU - Ohuchi,Atsushi, AU - Kitahara,Takashi, AU - Visscher,Marty O, AU - Takema,Yoshinori, AU - Tsuboi,Ryoji, AU - Boissy,Raymond E, PY - 2009/2/14/entrez PY - 2009/2/14/pubmed PY - 2009/6/16/medline SP - 30 EP - 9 JF - The Journal of pathology JO - J Pathol VL - 218 IS - 1 N2 - Hair greying is one of the most distinct but least comprehended features of senescence. The signalling of stem cell factor (SCF) and its receptor KIT has been documented to regulate essential roles in the maintenance of embryonic melanocyte lineages and postnatal cutaneous melanogenesis, although little is known about its detailed mechanisms in postnatal hair pigmentation. To address this, anagen human hair follicles and C57BL/6 murine pelage were analysed in this study. Molecular biological analyses of murine follicular skin indicated a significant increase of membrane-bound SCF expression, reaching its peak 8-16 days after anagen induction in concert with the escalation of cutaneous tyrosinase activity and corresponding pigmentation. Administration of KIT-neutralizing antibody abolished MITF and tyrosinase expressions, resulting in a reversible hair depigmentation in murine regenerated hair and human hair organ culture. Quantitative RT-PCR of human hair follicles indicated that KIT expression as well as the expression of several melanogenic factors, including MITF, was significantly lower in unpigmented than in pigmented follicles. Taken together, these data revealed a pivotal role of SCF-KIT signalling in the maintenance of human hair follicle melanogenesis during the anagen cycle and its involvement in physiological ageing of the hair follicle pigmentary unit. SN - 1096-9896 UR - https://www.unboundmedicine.com/medline/citation/19214986/Stem_cell_factor_KIT_signalling_plays_a_pivotal_role_in_regulating_pigmentation_in_mammalian_hair_ L2 - https://doi.org/10.1002/path.2503 DB - PRIME DP - Unbound Medicine ER -