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A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice.
BMC Infect Dis. 2009 Feb 12; 9:17.BI

Abstract

BACKGROUND

Developing vaccines for the prevention of human infection by H5N1 influenza viruses is an urgent task. DNA vaccines are a novel alternative to conventional vaccines and should contribute to the prophylaxis of emerging H5N1 virus. In this study, we assessed whether a single immunization with plasmid DNA expressing H5N1 hemagglutinin (HA) could provide early protection against lethal challenge in a mouse model.

METHODS

Mice were immunized once with HA DNA at 3, 5, 7 days before a lethal challenge. The survival rate, virus titer in the lungs and change of body weight were assayed to evaluate the protective abilities of the vaccine. To test the humoral immune response induced by HA DNA, serum samples were collected through the eye canthus of mice on various days after immunization and examined for specific antibodies by ELISA and an HI assay. Splenocytes were isolated after the immunization to determine the antigen-specific T-cell response by the ELISPOT assay.

RESULTS

Challenge experiments revealed that a single immunization of H5N1 virus HA DNA is effective in early protection against lethal homologous virus. Immunological analysis showed that an antigen-specific antibody and T-cell response could be elicited in mice shortly after the immunization. The protective abilities were correlated with the amount of injected DNA and the length of time after vaccination.

CONCLUSION

A single immunization of 100 mug H5 HA DNA vaccine combined with electroporation was able to provide early protection in mice against homologous virus infection.

Authors+Show Affiliations

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China. zlyfxz981@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19216752

Citation

Zheng, Liyun, et al. "A Single Immunization With HA DNA Vaccine By Electroporation Induces Early Protection Against H5N1 Avian Influenza Virus Challenge in Mice." BMC Infectious Diseases, vol. 9, 2009, p. 17.
Zheng L, Wang F, Yang Z, et al. A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice. BMC Infect Dis. 2009;9:17.
Zheng, L., Wang, F., Yang, Z., Chen, J., Chang, H., & Chen, Z. (2009). A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice. BMC Infectious Diseases, 9, 17. https://doi.org/10.1186/1471-2334-9-17
Zheng L, et al. A Single Immunization With HA DNA Vaccine By Electroporation Induces Early Protection Against H5N1 Avian Influenza Virus Challenge in Mice. BMC Infect Dis. 2009 Feb 12;9:17. PubMed PMID: 19216752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice. AU - Zheng,Liyun, AU - Wang,Fuyan, AU - Yang,Zhongdong, AU - Chen,Jianjun, AU - Chang,Haiyan, AU - Chen,Ze, Y1 - 2009/02/12/ PY - 2008/05/30/received PY - 2009/02/12/accepted PY - 2009/2/17/entrez PY - 2009/2/17/pubmed PY - 2009/3/13/medline SP - 17 EP - 17 JF - BMC infectious diseases JO - BMC Infect Dis VL - 9 N2 - BACKGROUND: Developing vaccines for the prevention of human infection by H5N1 influenza viruses is an urgent task. DNA vaccines are a novel alternative to conventional vaccines and should contribute to the prophylaxis of emerging H5N1 virus. In this study, we assessed whether a single immunization with plasmid DNA expressing H5N1 hemagglutinin (HA) could provide early protection against lethal challenge in a mouse model. METHODS: Mice were immunized once with HA DNA at 3, 5, 7 days before a lethal challenge. The survival rate, virus titer in the lungs and change of body weight were assayed to evaluate the protective abilities of the vaccine. To test the humoral immune response induced by HA DNA, serum samples were collected through the eye canthus of mice on various days after immunization and examined for specific antibodies by ELISA and an HI assay. Splenocytes were isolated after the immunization to determine the antigen-specific T-cell response by the ELISPOT assay. RESULTS: Challenge experiments revealed that a single immunization of H5N1 virus HA DNA is effective in early protection against lethal homologous virus. Immunological analysis showed that an antigen-specific antibody and T-cell response could be elicited in mice shortly after the immunization. The protective abilities were correlated with the amount of injected DNA and the length of time after vaccination. CONCLUSION: A single immunization of 100 mug H5 HA DNA vaccine combined with electroporation was able to provide early protection in mice against homologous virus infection. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/19216752/A_single_immunization_with_HA_DNA_vaccine_by_electroporation_induces_early_protection_against_H5N1_avian_influenza_virus_challenge_in_mice_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-9-17 DB - PRIME DP - Unbound Medicine ER -