TY - JOUR T1 - Determination of alprostadil in rat plasma by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry after intravenous administration. AU - Lin,Xia, AU - Zhang,Yu, AU - Cui,Yue, AU - Wang,Lin, AU - Wang,Jing, AU - Tang,Xing, Y1 - 2009/01/21/ PY - 2008/11/14/received PY - 2009/01/10/revised PY - 2009/01/12/accepted PY - 2009/2/17/entrez PY - 2009/2/17/pubmed PY - 2009/7/17/medline SP - 983 EP - 8 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 49 IS - 4 N2 - A rapid, highly selective ultra performance liquid chromatography-electrospray ionisation-tandem mass spectrometry method (UPLC-ESI-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of alprostadil in rat plasma. After a simple sample preparation procedure involving a one-step liquid-liquid extraction, alprostadil and the internal standard, diphenhydramine, were chromatographed on an ACQUITY UPLC BEH C(18) column with gradient elution using a mobile phase consisting of acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.25 mL min(-1). The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode via an electrospray ionization (ESI) source. The calibration curve was linear (r(2)=0.99) over the concentration range 0.4-250.0 ng mL(-1), with a lower limit of quantification of 0.4 ng mL(-1) for alprostadil. The inter- and intra-day precision (%R.S.D.) was less than 8.5% and 2.4%, respectively, and the accuracy (RE%) was between 9.3% and 1.0% (n=6). Alprostadil in rat plasma was stable when stored at room temperature for 0.5h and at -20 degrees C for two weeks. The method was very rapid, simple and reliable, and was employed for the first time for the pharmacokinetic studies of alprostadil in rats after a single intravenous administration of 50 microg kg(-1). SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19217739/Determination_of_alprostadil_in_rat_plasma_by_ultra_performance_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_after_intravenous_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(09)00040-5 DB - PRIME DP - Unbound Medicine ER -