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Apolipoprotein E polymorphism and expression in type 2 diabetic patients with nephropathy: clinicopathological correlation.
Nephrol Dial Transplant. 2009 Jun; 24(6):1889-95.ND

Abstract

BACKGROUND

Diabetic nephropathy represents a heterogeneous group of renal pathologies that may be associated with genetic susceptibility. There have been clinical reports on the risk association of diabetic nephropathy with an apolipoprotein E (ApoE) exon 4 polymorphism although its correlations with renal histopathological changes have not been explored.

METHODS

A total of 213 adult autopsies with type 2 diabetes and 111 non-diabetic control cases were analysed. Genomic DNA samples were obtained from spleen tissues. The ApoE genotype was determined by PCR-LDR analysis. Histopathological examination of kidney sections was performed in a subset of 51 diabetic and 111 control cases. ApoE protein expression in diabetic carriers with similar clinical status was examined by immunohistochemical staining.

RESULTS

In type 2 diabetes, epsilon2 carriers (P = 0.04; odds ratio = 5.42; 95% CI: 1.10-26.8) and epsilon3/epsilon4 (P = 0.04; odds ratio = 22.5; 95% CI: 1.11-454.90) genotype carriers were more likely to have glomerular hypertrophy than were epsilon3/epsilon3 carriers. The epsilon2 carriers showed an increase in glomerular ApoE protein expression. A correlation between ApoE genotype and nodular glomerulosclerosis was not found.

CONCLUSIONS

Our findings confirm the risk association of the ApoE polymorphism with diabetic nephropathy in clinical studies and is the first study demonstrating the correlations between ApoE genotypes, protein expression and structural changes in diabetic nephropathy.

Authors+Show Affiliations

Department of Medicine and Therapeutics, The Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19218599

Citation

Guan, Jing, et al. "Apolipoprotein E Polymorphism and Expression in Type 2 Diabetic Patients With Nephropathy: Clinicopathological Correlation." Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association, vol. 24, no. 6, 2009, pp. 1889-95.
Guan J, Zhao HL, Baum L, et al. Apolipoprotein E polymorphism and expression in type 2 diabetic patients with nephropathy: clinicopathological correlation. Nephrol Dial Transplant. 2009;24(6):1889-95.
Guan, J., Zhao, H. L., Baum, L., Sui, Y., He, L., Wong, H., Lai, F. M., Tong, P. C., & Chan, J. C. (2009). Apolipoprotein E polymorphism and expression in type 2 diabetic patients with nephropathy: clinicopathological correlation. Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association, 24(6), 1889-95. https://doi.org/10.1093/ndt/gfn734
Guan J, et al. Apolipoprotein E Polymorphism and Expression in Type 2 Diabetic Patients With Nephropathy: Clinicopathological Correlation. Nephrol Dial Transplant. 2009;24(6):1889-95. PubMed PMID: 19218599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apolipoprotein E polymorphism and expression in type 2 diabetic patients with nephropathy: clinicopathological correlation. AU - Guan,Jing, AU - Zhao,Hai-Lu, AU - Baum,Larry, AU - Sui,Yi, AU - He,Lan, AU - Wong,Harriet, AU - Lai,Fernand M M, AU - Tong,Peter C Y, AU - Chan,Juliana C N, Y1 - 2009/02/14/ PY - 2009/2/17/entrez PY - 2009/2/17/pubmed PY - 2009/8/26/medline SP - 1889 EP - 95 JF - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association JO - Nephrol Dial Transplant VL - 24 IS - 6 N2 - BACKGROUND: Diabetic nephropathy represents a heterogeneous group of renal pathologies that may be associated with genetic susceptibility. There have been clinical reports on the risk association of diabetic nephropathy with an apolipoprotein E (ApoE) exon 4 polymorphism although its correlations with renal histopathological changes have not been explored. METHODS: A total of 213 adult autopsies with type 2 diabetes and 111 non-diabetic control cases were analysed. Genomic DNA samples were obtained from spleen tissues. The ApoE genotype was determined by PCR-LDR analysis. Histopathological examination of kidney sections was performed in a subset of 51 diabetic and 111 control cases. ApoE protein expression in diabetic carriers with similar clinical status was examined by immunohistochemical staining. RESULTS: In type 2 diabetes, epsilon2 carriers (P = 0.04; odds ratio = 5.42; 95% CI: 1.10-26.8) and epsilon3/epsilon4 (P = 0.04; odds ratio = 22.5; 95% CI: 1.11-454.90) genotype carriers were more likely to have glomerular hypertrophy than were epsilon3/epsilon3 carriers. The epsilon2 carriers showed an increase in glomerular ApoE protein expression. A correlation between ApoE genotype and nodular glomerulosclerosis was not found. CONCLUSIONS: Our findings confirm the risk association of the ApoE polymorphism with diabetic nephropathy in clinical studies and is the first study demonstrating the correlations between ApoE genotypes, protein expression and structural changes in diabetic nephropathy. SN - 1460-2385 UR - https://www.unboundmedicine.com/medline/citation/19218599/Apolipoprotein_E_polymorphism_and_expression_in_type_2_diabetic_patients_with_nephropathy:_clinicopathological_correlation_ L2 - https://academic.oup.com/ndt/article-lookup/doi/10.1093/ndt/gfn734 DB - PRIME DP - Unbound Medicine ER -