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Evaluation of FITC-induced atopic dermatitis-like disease in NC/Nga mice and BALB/c mice using computer-assisted stereological toolbox, a computer-aided morphometric system.
Int Arch Allergy Immunol. 2009; 149(3):188-94.IA

Abstract

BACKGROUND

The NC/Nga mouse spontaneously develops eczematous atopic dermatitis (AD)-like skin lesions when maintained under conventional conditions, but not when kept under specific pathogen-free (SPF) conditions. Hence, there is a need for an AD model in mice housed under SPF conditions, as this is mandatory for research animals in many countries.

METHODS

We evaluated the use of the hapten FITC as an inducer of AD-like disease in NC/Nga and BALB/c mice maintained under SPF conditions. Mice were either untreated or treated with tacrolimus or betamethasone. Using the software Computer Assisted Stereological Toolbox as a stereological method, the mice were sensitized to FITC and the histological efficiency of disease induction with regard to inflammation and CD4+ and CD8+ lymphocytes, in addition to mast cells, was evaluated. The method was validated by comparison to a conventional semiquantitative observer-dependent method.

RESULTS

Our findings prove that FITC does indeed induce AD-like lesions in NC/Nga mice with regard to the histological appearance of the mice. However, when evaluating the immunological response in the affected areas of the mice with regard to the CD4/CD8 ratio and the effect of treatment, we found that the immune response in the NC/Nga mice differed from AD skin lesions in humans in certain aspects.

CONCLUSIONS

These results emphasize the importance of an assessment of not only the histological but also the immunological appearance of the skin when evaluating AD-like disease in mice as a model for AD in humans.

Authors+Show Affiliations

Department of Dermato-Venereology, Aarhus University Hospital, Aarhus, Denmark. malenel@medmicro.au.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19218811

Citation

Hvid, Malene, et al. "Evaluation of FITC-induced Atopic Dermatitis-like Disease in NC/Nga Mice and BALB/c Mice Using Computer-assisted Stereological Toolbox, a Computer-aided Morphometric System." International Archives of Allergy and Immunology, vol. 149, no. 3, 2009, pp. 188-94.
Hvid M, Jensen HK, Deleuran B, et al. Evaluation of FITC-induced atopic dermatitis-like disease in NC/Nga mice and BALB/c mice using computer-assisted stereological toolbox, a computer-aided morphometric system. Int Arch Allergy Immunol. 2009;149(3):188-94.
Hvid, M., Jensen, H. K., Deleuran, B., Kemp, K., Andersson, C., Deleuran, M., & Vestergaard, C. (2009). Evaluation of FITC-induced atopic dermatitis-like disease in NC/Nga mice and BALB/c mice using computer-assisted stereological toolbox, a computer-aided morphometric system. International Archives of Allergy and Immunology, 149(3), 188-94. https://doi.org/10.1159/000199714
Hvid M, et al. Evaluation of FITC-induced Atopic Dermatitis-like Disease in NC/Nga Mice and BALB/c Mice Using Computer-assisted Stereological Toolbox, a Computer-aided Morphometric System. Int Arch Allergy Immunol. 2009;149(3):188-94. PubMed PMID: 19218811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of FITC-induced atopic dermatitis-like disease in NC/Nga mice and BALB/c mice using computer-assisted stereological toolbox, a computer-aided morphometric system. AU - Hvid,Malene, AU - Jensen,Helene Kofoed, AU - Deleuran,Bent, AU - Kemp,Kaare, AU - Andersson,Christina, AU - Deleuran,Mette, AU - Vestergaard,Christian, Y1 - 2009/02/12/ PY - 2008/06/24/received PY - 2008/10/10/accepted PY - 2009/2/17/entrez PY - 2009/2/17/pubmed PY - 2009/7/22/medline SP - 188 EP - 94 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 149 IS - 3 N2 - BACKGROUND: The NC/Nga mouse spontaneously develops eczematous atopic dermatitis (AD)-like skin lesions when maintained under conventional conditions, but not when kept under specific pathogen-free (SPF) conditions. Hence, there is a need for an AD model in mice housed under SPF conditions, as this is mandatory for research animals in many countries. METHODS: We evaluated the use of the hapten FITC as an inducer of AD-like disease in NC/Nga and BALB/c mice maintained under SPF conditions. Mice were either untreated or treated with tacrolimus or betamethasone. Using the software Computer Assisted Stereological Toolbox as a stereological method, the mice were sensitized to FITC and the histological efficiency of disease induction with regard to inflammation and CD4+ and CD8+ lymphocytes, in addition to mast cells, was evaluated. The method was validated by comparison to a conventional semiquantitative observer-dependent method. RESULTS: Our findings prove that FITC does indeed induce AD-like lesions in NC/Nga mice with regard to the histological appearance of the mice. However, when evaluating the immunological response in the affected areas of the mice with regard to the CD4/CD8 ratio and the effect of treatment, we found that the immune response in the NC/Nga mice differed from AD skin lesions in humans in certain aspects. CONCLUSIONS: These results emphasize the importance of an assessment of not only the histological but also the immunological appearance of the skin when evaluating AD-like disease in mice as a model for AD in humans. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/19218811/Evaluation_of_FITC_induced_atopic_dermatitis_like_disease_in_NC/Nga_mice_and_BALB/c_mice_using_computer_assisted_stereological_toolbox_a_computer_aided_morphometric_system_ L2 - https://www.karger.com?DOI=10.1159/000199714 DB - PRIME DP - Unbound Medicine ER -