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Comparison of acute inflammatory and chronic structural asthma-like responses between C57BL/6 and BALB/c mice.
Int Arch Allergy Immunol. 2009; 149(3):195-207.IA

Abstract

BACKGROUND

The interactions between airway responsiveness, structural remodelling and inflammation in allergic asthma remain poorly understood. Prolonged challenge with inhaled allergen is necessary to replicate many of the features of airway wall remodelling in mice. In both mice and humans, genetic differences can have a profound influence on allergy, inflammation, airway responsiveness and structural changes.

METHODS

The aim of this study was to provide a comparative analysis of allergen-induced airway changes in sensitized BALB/c and C57BL/6 mice that were exposed to inhaled allergen for 2 ('acute'), 6 or 9 weeks ('chronic'). Inflammation, remodelling and responsiveness were analyzed.

RESULTS

Both strains developed a Th-2-driven airway inflammation with allergen-specific IgE, airway eosinophilia and goblet cell hyperplasia upon 2 weeks of allergen inhalation. This was accompanied by a significant increase in airway smooth muscle mass and hyperresponsiveness in BALB/c but not in C57BL/6 mice. However, airway eosinophilia was more pronounced in the C57BL/6 strain. Chronic allergen exposure (6 or 9 weeks) resulted in an increase in airway smooth muscle mass as well as subepithelial collagen and fibronectin deposition in both strains. The emergence of these structural changes paralleled the disappearance of inflammation in both C57BL/6 and BALB/c mice and loss of hyperresponsiveness in the BALB/c strain. TGF-beta(1)was accordingly elevated in both strains.

CONCLUSION

Airway inflammation, remodelling and hyperresponsiveness are closely intertwined processes. Genetic background influences several aspects of the acute allergic phenotype. Chronic allergen exposure induces a marked airway remodelling that parallels a decreased inflammation, which was largely comparable between the two strains.

Authors+Show Affiliations

Department of Respiratory Medicine, Ghent University, Ghent, Belgium. chris.vanhove@UGent.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19218812

Citation

Van Hove, Chris L., et al. "Comparison of Acute Inflammatory and Chronic Structural Asthma-like Responses Between C57BL/6 and BALB/c Mice." International Archives of Allergy and Immunology, vol. 149, no. 3, 2009, pp. 195-207.
Van Hove CL, Maes T, Cataldo DD, et al. Comparison of acute inflammatory and chronic structural asthma-like responses between C57BL/6 and BALB/c mice. Int Arch Allergy Immunol. 2009;149(3):195-207.
Van Hove, C. L., Maes, T., Cataldo, D. D., Guéders, M. M., Palmans, E., Joos, G. F., & Tournoy, K. G. (2009). Comparison of acute inflammatory and chronic structural asthma-like responses between C57BL/6 and BALB/c mice. International Archives of Allergy and Immunology, 149(3), 195-207. https://doi.org/10.1159/000199715
Van Hove CL, et al. Comparison of Acute Inflammatory and Chronic Structural Asthma-like Responses Between C57BL/6 and BALB/c Mice. Int Arch Allergy Immunol. 2009;149(3):195-207. PubMed PMID: 19218812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of acute inflammatory and chronic structural asthma-like responses between C57BL/6 and BALB/c mice. AU - Van Hove,Chris L, AU - Maes,Tania, AU - Cataldo,Didier D, AU - Guéders,Maud M, AU - Palmans,Els, AU - Joos,Guy F, AU - Tournoy,Kurt G, Y1 - 2009/02/12/ PY - 2008/05/07/received PY - 2008/09/30/accepted PY - 2009/2/17/entrez PY - 2009/2/17/pubmed PY - 2009/7/22/medline SP - 195 EP - 207 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 149 IS - 3 N2 - BACKGROUND: The interactions between airway responsiveness, structural remodelling and inflammation in allergic asthma remain poorly understood. Prolonged challenge with inhaled allergen is necessary to replicate many of the features of airway wall remodelling in mice. In both mice and humans, genetic differences can have a profound influence on allergy, inflammation, airway responsiveness and structural changes. METHODS: The aim of this study was to provide a comparative analysis of allergen-induced airway changes in sensitized BALB/c and C57BL/6 mice that were exposed to inhaled allergen for 2 ('acute'), 6 or 9 weeks ('chronic'). Inflammation, remodelling and responsiveness were analyzed. RESULTS: Both strains developed a Th-2-driven airway inflammation with allergen-specific IgE, airway eosinophilia and goblet cell hyperplasia upon 2 weeks of allergen inhalation. This was accompanied by a significant increase in airway smooth muscle mass and hyperresponsiveness in BALB/c but not in C57BL/6 mice. However, airway eosinophilia was more pronounced in the C57BL/6 strain. Chronic allergen exposure (6 or 9 weeks) resulted in an increase in airway smooth muscle mass as well as subepithelial collagen and fibronectin deposition in both strains. The emergence of these structural changes paralleled the disappearance of inflammation in both C57BL/6 and BALB/c mice and loss of hyperresponsiveness in the BALB/c strain. TGF-beta(1)was accordingly elevated in both strains. CONCLUSION: Airway inflammation, remodelling and hyperresponsiveness are closely intertwined processes. Genetic background influences several aspects of the acute allergic phenotype. Chronic allergen exposure induces a marked airway remodelling that parallels a decreased inflammation, which was largely comparable between the two strains. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/19218812/Comparison_of_acute_inflammatory_and_chronic_structural_asthma_like_responses_between_C57BL/6_and_BALB/c_mice_ DB - PRIME DP - Unbound Medicine ER -