Tags

Type your tag names separated by a space and hit enter

Therapeutic effects of rectal administration of muscovite on experimental colitis in rats.
J Gastroenterol Hepatol. 2009 May; 24(5):912-9.JG

Abstract

BACKGROUND AND AIMS

The aim of this study was to investigate whether rectal administration of muscovite can ameliorate colonic inflammation in a rat model of experimental colitis, and its possible mechanism.

METHODS

Female Sprague-Dawley (SD) rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis were treated with rectal administration of muscovite or 5-aminosalicylic acid (5-ASA) daily for 14 days. The changes in body weight, macroscopic damage and histologic scores were subsequently evaluated. Gene expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), mucin2 (MUC2) and trefoil factor 3 (TFF3) in the colonic tissues was assessed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) while protein levels of TNF-alpha and IL-1beta were detected by ELISA. Mucin2 expression in colonic mucosa was detected by immunohistochemistry. The capacity of muscovite to adsorb cytokines in vitro was determined by the changes in the amount of TNF-alpha, IL-1beta secreted by lipopolysaccharide (LPS)-stimulated THP-1 cells and IL-8 secreted by LPS-stimulated HT-29 cells.

RESULTS

Rectal administration of muscovite improved the loss of body weight, macroscopic and histologic scores of TNBS-induced colitis in a dose-dependent manner. Trinitrobenzene sulfonic acid-induced expression of TNF-alpha and IL-1beta was reduced by muscovite and 5-ASA treatment. Reduction of MUC2 expression in colitis rats was reversed by muscovite and 5-ASA treatment. However, the expression of TFF3 mRNA in colonic mucosa was not affected. In addition, we found muscovite inhibited the expression of TNF-alpha, IL-1beta secreted by THP-1 and IL-8 secreted by HT-29 cells in a dose-dependent manner.

CONCLUSIONS

Our study demonstrated for the first time that rectal administration of muscovite can ameliorate colonic inflammation of TNBS-induced colitis. Further confirmatory studies are needed to prove that muscovite might be a potential therapeutic agent for the treatment of ulcerative colitis.

Authors+Show Affiliations

Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19220658

Citation

Chen, Yan, et al. "Therapeutic Effects of Rectal Administration of Muscovite On Experimental Colitis in Rats." Journal of Gastroenterology and Hepatology, vol. 24, no. 5, 2009, pp. 912-9.
Chen Y, Chen Y, Liu WL, et al. Therapeutic effects of rectal administration of muscovite on experimental colitis in rats. J Gastroenterol Hepatol. 2009;24(5):912-9.
Chen, Y., Chen, Y., Liu, W. L., Zhou, T. H., Cai, J. T., Du, Q., Wang, L. J., Wu, J. G., & Si, J. M. (2009). Therapeutic effects of rectal administration of muscovite on experimental colitis in rats. Journal of Gastroenterology and Hepatology, 24(5), 912-9. https://doi.org/10.1111/j.1440-1746.2008.05721.x
Chen Y, et al. Therapeutic Effects of Rectal Administration of Muscovite On Experimental Colitis in Rats. J Gastroenterol Hepatol. 2009;24(5):912-9. PubMed PMID: 19220658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic effects of rectal administration of muscovite on experimental colitis in rats. AU - Chen,Yan, AU - Chen,Yi, AU - Liu,Wei-li, AU - Zhou,Tian-hua, AU - Cai,Jian-ting, AU - Du,Qing, AU - Wang,Liang-jing, AU - Wu,Jia-Guo, AU - Si,Jian-min, Y1 - 2009/02/09/ PY - 2009/2/18/entrez PY - 2009/2/18/pubmed PY - 2009/10/16/medline SP - 912 EP - 9 JF - Journal of gastroenterology and hepatology JO - J. Gastroenterol. Hepatol. VL - 24 IS - 5 N2 - BACKGROUND AND AIMS: The aim of this study was to investigate whether rectal administration of muscovite can ameliorate colonic inflammation in a rat model of experimental colitis, and its possible mechanism. METHODS: Female Sprague-Dawley (SD) rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis were treated with rectal administration of muscovite or 5-aminosalicylic acid (5-ASA) daily for 14 days. The changes in body weight, macroscopic damage and histologic scores were subsequently evaluated. Gene expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), mucin2 (MUC2) and trefoil factor 3 (TFF3) in the colonic tissues was assessed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) while protein levels of TNF-alpha and IL-1beta were detected by ELISA. Mucin2 expression in colonic mucosa was detected by immunohistochemistry. The capacity of muscovite to adsorb cytokines in vitro was determined by the changes in the amount of TNF-alpha, IL-1beta secreted by lipopolysaccharide (LPS)-stimulated THP-1 cells and IL-8 secreted by LPS-stimulated HT-29 cells. RESULTS: Rectal administration of muscovite improved the loss of body weight, macroscopic and histologic scores of TNBS-induced colitis in a dose-dependent manner. Trinitrobenzene sulfonic acid-induced expression of TNF-alpha and IL-1beta was reduced by muscovite and 5-ASA treatment. Reduction of MUC2 expression in colitis rats was reversed by muscovite and 5-ASA treatment. However, the expression of TFF3 mRNA in colonic mucosa was not affected. In addition, we found muscovite inhibited the expression of TNF-alpha, IL-1beta secreted by THP-1 and IL-8 secreted by HT-29 cells in a dose-dependent manner. CONCLUSIONS: Our study demonstrated for the first time that rectal administration of muscovite can ameliorate colonic inflammation of TNBS-induced colitis. Further confirmatory studies are needed to prove that muscovite might be a potential therapeutic agent for the treatment of ulcerative colitis. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/19220658/Therapeutic_effects_of_rectal_administration_of_muscovite_on_experimental_colitis_in_rats_ L2 - https://doi.org/10.1111/j.1440-1746.2008.05721.x DB - PRIME DP - Unbound Medicine ER -