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Dynamic 13C-tracer study of storage carbohydrate pools in aerobic glucose-limited Saccharomyces cerevisiae confirms a rapid steady-state turnover and fast mobilization during a modest stepup in the glucose uptake rate.
FEMS Yeast Res. 2009 Mar; 9(2):191-201.FY

Abstract

In this research, two dynamic (13)C-labeling experiments confirmed turnover and rapid mobilization of stored glycogen and trehalose in an aerobic glucose-limited chemostat (D=0.05 h(-1)) culture of Saccharomyces cerevisiae. In one experiment, the continuous feed to an aerobic glucose-limited chemostat culture of S. cerevisiae was instantaneously switched from naturally labeled to fully (13)C labeled while maintaining the same feed rate before and after the switch. The dynamic replacements of naturally labeled intracellular glycolytic intermediates and CO(2) (in the off-gas) with their (13)C-labeled equivalents were measured. The data of this experiment suggest that the continuous turnover of glycogen and trehalose is substantial (c. 1/3 of the glycolytic flux). The second experiment combined the medium switch with a shiftup in the glucose feeding rate (dilution rate shiftup from 0.05 to 0.10 h(-1)). This experiment triggered a strong but transient mobilization of storage carbon, that was channelled into glycolysis, causing a significant disruption in the dynamic labeling profile of glycolytic intermediates. The off-gas measurements in the shiftup experiment confirmed a considerable transient influx of (12)C-carbon into glycolysis after the combined medium switch and dilution rate shiftup. This study shows that for accurate in vivo kinetic interpretation of rapid pulse experiments, glycogen and trehalose metabolism must be taken into account.

Authors+Show Affiliations

Department of Biotechnology, Delft University of Technology, Delft, The Netherlands. f.o.aboka@tudelft.nlNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19220865

Citation

Aboka, Fredrick O., et al. "Dynamic 13C-tracer Study of Storage Carbohydrate Pools in Aerobic Glucose-limited Saccharomyces Cerevisiae Confirms a Rapid Steady-state Turnover and Fast Mobilization During a Modest Stepup in the Glucose Uptake Rate." FEMS Yeast Research, vol. 9, no. 2, 2009, pp. 191-201.
Aboka FO, Heijnen JJ, van Winden WA. Dynamic 13C-tracer study of storage carbohydrate pools in aerobic glucose-limited Saccharomyces cerevisiae confirms a rapid steady-state turnover and fast mobilization during a modest stepup in the glucose uptake rate. FEMS Yeast Res. 2009;9(2):191-201.
Aboka, F. O., Heijnen, J. J., & van Winden, W. A. (2009). Dynamic 13C-tracer study of storage carbohydrate pools in aerobic glucose-limited Saccharomyces cerevisiae confirms a rapid steady-state turnover and fast mobilization during a modest stepup in the glucose uptake rate. FEMS Yeast Research, 9(2), 191-201. https://doi.org/10.1111/j.1567-1364.2008.00465.x
Aboka FO, Heijnen JJ, van Winden WA. Dynamic 13C-tracer Study of Storage Carbohydrate Pools in Aerobic Glucose-limited Saccharomyces Cerevisiae Confirms a Rapid Steady-state Turnover and Fast Mobilization During a Modest Stepup in the Glucose Uptake Rate. FEMS Yeast Res. 2009;9(2):191-201. PubMed PMID: 19220865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic 13C-tracer study of storage carbohydrate pools in aerobic glucose-limited Saccharomyces cerevisiae confirms a rapid steady-state turnover and fast mobilization during a modest stepup in the glucose uptake rate. AU - Aboka,Fredrick O, AU - Heijnen,Joseph J, AU - van Winden,Wouter A, PY - 2009/2/18/entrez PY - 2009/2/18/pubmed PY - 2009/3/21/medline SP - 191 EP - 201 JF - FEMS yeast research JO - FEMS Yeast Res VL - 9 IS - 2 N2 - In this research, two dynamic (13)C-labeling experiments confirmed turnover and rapid mobilization of stored glycogen and trehalose in an aerobic glucose-limited chemostat (D=0.05 h(-1)) culture of Saccharomyces cerevisiae. In one experiment, the continuous feed to an aerobic glucose-limited chemostat culture of S. cerevisiae was instantaneously switched from naturally labeled to fully (13)C labeled while maintaining the same feed rate before and after the switch. The dynamic replacements of naturally labeled intracellular glycolytic intermediates and CO(2) (in the off-gas) with their (13)C-labeled equivalents were measured. The data of this experiment suggest that the continuous turnover of glycogen and trehalose is substantial (c. 1/3 of the glycolytic flux). The second experiment combined the medium switch with a shiftup in the glucose feeding rate (dilution rate shiftup from 0.05 to 0.10 h(-1)). This experiment triggered a strong but transient mobilization of storage carbon, that was channelled into glycolysis, causing a significant disruption in the dynamic labeling profile of glycolytic intermediates. The off-gas measurements in the shiftup experiment confirmed a considerable transient influx of (12)C-carbon into glycolysis after the combined medium switch and dilution rate shiftup. This study shows that for accurate in vivo kinetic interpretation of rapid pulse experiments, glycogen and trehalose metabolism must be taken into account. SN - 1567-1364 UR - https://www.unboundmedicine.com/medline/citation/19220865/Dynamic_13C_tracer_study_of_storage_carbohydrate_pools_in_aerobic_glucose_limited_Saccharomyces_cerevisiae_confirms_a_rapid_steady_state_turnover_and_fast_mobilization_during_a_modest_stepup_in_the_glucose_uptake_rate_ DB - PRIME DP - Unbound Medicine ER -