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Effect of dietary fatty acid composition on Th1/Th2 polarization in lymphocytes.
JPEN J Parenter Enteral Nutr 2009 Jul-Aug; 33(4):390-6JJ

Abstract

BACKGROUND

It has become increasingly clear that polyunsaturated fatty acids (PUFAs) have immunomodulatory effects. However, the intake of these fatty acids used in animal studies often greatly exceeds dietary human intake. Whether differences in the composition of fatty acids that are consumed in amounts consistent with normal dietary intake can influence immune function remains uncertain.

METHODS

We manufactured 3 types of liquid diet, related to modified fatty acid composition (omega-6/omega-3 = 0.25, 2.27 and 42.9), but excluding eicosapentaenoic acid and docosahexaenoic acid, based upon a liquid diet used clinically in humans. We assessed CD3-stimulated cytokine production of splenocytes in female BALB/c mice (n = 4 per group) fed 1 of 3 liquid diets for 4 weeks. We also measured the cytokine production of peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin in humans at the end of a 4-week period of consumption of 2 different liquid diets (omega-6/omega-3 = 3 and 44).

RESULTS

We found that the ratio of interfero omega-gamma (IFN-gamma) / interleukin-4 (IL-4) was significantly higher in mice fed the omega-3 rich diet than in others. In humans, IFN-gamma / IL-4 was significantly higher after the omega-3 versus the omega-6 enhanced diet.

CONCLUSIONS

Differences in the composition of omega-3 and omega-6 PUFAs induces a shift in the Th1/Th2 balance in both mouse and human lymphocytes, even when ingested in normal dietary amounts. An omega-3 rich diet containing alpha-linolenic acid modulates immune function.

Authors+Show Affiliations

Department of Clinical Neurosciences, Nagasaki University Graduate School of Biomedical Sciences, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19221048

Citation

Mizota, Takamitsu, et al. "Effect of Dietary Fatty Acid Composition On Th1/Th2 Polarization in Lymphocytes." JPEN. Journal of Parenteral and Enteral Nutrition, vol. 33, no. 4, 2009, pp. 390-6.
Mizota T, Fujita-Kambara C, Matsuya N, et al. Effect of dietary fatty acid composition on Th1/Th2 polarization in lymphocytes. JPEN J Parenter Enteral Nutr. 2009;33(4):390-6.
Mizota, T., Fujita-Kambara, C., Matsuya, N., Hamasaki, S., Fukudome, T., Goto, H., ... Matsuo, H. (2009). Effect of dietary fatty acid composition on Th1/Th2 polarization in lymphocytes. JPEN. Journal of Parenteral and Enteral Nutrition, 33(4), pp. 390-6. doi:10.1177/0148607108325252.
Mizota T, et al. Effect of Dietary Fatty Acid Composition On Th1/Th2 Polarization in Lymphocytes. JPEN J Parenter Enteral Nutr. 2009;33(4):390-6. PubMed PMID: 19221048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary fatty acid composition on Th1/Th2 polarization in lymphocytes. AU - Mizota,Takamitsu, AU - Fujita-Kambara,Chiaki, AU - Matsuya,Nemu, AU - Hamasaki,Shinji, AU - Fukudome,Takayasu, AU - Goto,Hirofumi, AU - Nakane,Shunya, AU - Kondo,Takayuki, AU - Matsuo,Hidenori, Y1 - 2009/02/12/ PY - 2009/2/18/entrez PY - 2009/2/18/pubmed PY - 2009/8/8/medline SP - 390 EP - 6 JF - JPEN. Journal of parenteral and enteral nutrition JO - JPEN J Parenter Enteral Nutr VL - 33 IS - 4 N2 - BACKGROUND: It has become increasingly clear that polyunsaturated fatty acids (PUFAs) have immunomodulatory effects. However, the intake of these fatty acids used in animal studies often greatly exceeds dietary human intake. Whether differences in the composition of fatty acids that are consumed in amounts consistent with normal dietary intake can influence immune function remains uncertain. METHODS: We manufactured 3 types of liquid diet, related to modified fatty acid composition (omega-6/omega-3 = 0.25, 2.27 and 42.9), but excluding eicosapentaenoic acid and docosahexaenoic acid, based upon a liquid diet used clinically in humans. We assessed CD3-stimulated cytokine production of splenocytes in female BALB/c mice (n = 4 per group) fed 1 of 3 liquid diets for 4 weeks. We also measured the cytokine production of peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin in humans at the end of a 4-week period of consumption of 2 different liquid diets (omega-6/omega-3 = 3 and 44). RESULTS: We found that the ratio of interfero omega-gamma (IFN-gamma) / interleukin-4 (IL-4) was significantly higher in mice fed the omega-3 rich diet than in others. In humans, IFN-gamma / IL-4 was significantly higher after the omega-3 versus the omega-6 enhanced diet. CONCLUSIONS: Differences in the composition of omega-3 and omega-6 PUFAs induces a shift in the Th1/Th2 balance in both mouse and human lymphocytes, even when ingested in normal dietary amounts. An omega-3 rich diet containing alpha-linolenic acid modulates immune function. SN - 0148-6071 UR - https://www.unboundmedicine.com/medline/citation/19221048/Effect_of_dietary_fatty_acid_composition_on_Th1/Th2_polarization_in_lymphocytes_ L2 - https://doi.org/10.1177/0148607108325252 DB - PRIME DP - Unbound Medicine ER -