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Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae.
Appl Microbiol Biotechnol. 2009 Apr; 82(5):909-19.AM

Abstract

During growth of Saccharomyces cerevisiae on glucose, the redox cofactors NADH and NADPH are predominantly involved in catabolism and biosynthesis, respectively. A deviation from the optimal level of these cofactors often results in major changes in the substrate uptake and biomass formation. However, the metabolism of xylose by recombinant S. cerevisiae carrying xylose reductase and xylitol dehydrogenase from the fungal pathway requires both NADH and NADPH and creates cofactor imbalance during growth on xylose. As one possible solution to overcoming this imbalance, the effect of overexpressing the native NADH kinase (encoded by the POS5 gene) in xylose-consuming recombinant S. cerevisiae directed either into the cytosol or to the mitochondria was evaluated. The physiology of the NADH kinase containing strains was also evaluated during growth on glucose. Overexpressing NADH kinase in the cytosol redirected carbon flow from CO(2) to ethanol during aerobic growth on glucose and to ethanol and acetate during anaerobic growth on glucose. However, cytosolic NADH kinase has an opposite effect during anaerobic metabolism of xylose consumption by channeling carbon flow from ethanol to xylitol. In contrast, overexpressing NADH kinase in the mitochondria did not affect the physiology to a large extent. Overall, although NADH kinase did not increase the rate of xylose consumption, we believe that it can provide an important source of NADPH in yeast, which can be useful for metabolic engineering strategies where the redox fluxes are manipulated.

Authors+Show Affiliations

Center for Microbial Biotechnology, Technical University of Denmark, Building 223, 2800 Kgs., Lyngby, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19221731

Citation

Hou, Jin, et al. "Impact of Overexpressing NADH Kinase On Glucose and Xylose Metabolism in Recombinant Xylose-utilizing Saccharomyces Cerevisiae." Applied Microbiology and Biotechnology, vol. 82, no. 5, 2009, pp. 909-19.
Hou J, Vemuri GN, Bao X, et al. Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2009;82(5):909-19.
Hou, J., Vemuri, G. N., Bao, X., & Olsson, L. (2009). Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 82(5), 909-19. https://doi.org/10.1007/s00253-009-1900-4
Hou J, et al. Impact of Overexpressing NADH Kinase On Glucose and Xylose Metabolism in Recombinant Xylose-utilizing Saccharomyces Cerevisiae. Appl Microbiol Biotechnol. 2009;82(5):909-19. PubMed PMID: 19221731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae. AU - Hou,Jin, AU - Vemuri,Goutham N, AU - Bao,Xiaoming, AU - Olsson,Lisbeth, Y1 - 2009/02/17/ PY - 2008/12/08/received PY - 2009/01/25/accepted PY - 2009/01/23/revised PY - 2009/2/18/entrez PY - 2009/2/18/pubmed PY - 2009/5/14/medline SP - 909 EP - 19 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 82 IS - 5 N2 - During growth of Saccharomyces cerevisiae on glucose, the redox cofactors NADH and NADPH are predominantly involved in catabolism and biosynthesis, respectively. A deviation from the optimal level of these cofactors often results in major changes in the substrate uptake and biomass formation. However, the metabolism of xylose by recombinant S. cerevisiae carrying xylose reductase and xylitol dehydrogenase from the fungal pathway requires both NADH and NADPH and creates cofactor imbalance during growth on xylose. As one possible solution to overcoming this imbalance, the effect of overexpressing the native NADH kinase (encoded by the POS5 gene) in xylose-consuming recombinant S. cerevisiae directed either into the cytosol or to the mitochondria was evaluated. The physiology of the NADH kinase containing strains was also evaluated during growth on glucose. Overexpressing NADH kinase in the cytosol redirected carbon flow from CO(2) to ethanol during aerobic growth on glucose and to ethanol and acetate during anaerobic growth on glucose. However, cytosolic NADH kinase has an opposite effect during anaerobic metabolism of xylose consumption by channeling carbon flow from ethanol to xylitol. In contrast, overexpressing NADH kinase in the mitochondria did not affect the physiology to a large extent. Overall, although NADH kinase did not increase the rate of xylose consumption, we believe that it can provide an important source of NADPH in yeast, which can be useful for metabolic engineering strategies where the redox fluxes are manipulated. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/19221731/Impact_of_overexpressing_NADH_kinase_on_glucose_and_xylose_metabolism_in_recombinant_xylose_utilizing_Saccharomyces_cerevisiae_ L2 - https://dx.doi.org/10.1007/s00253-009-1900-4 DB - PRIME DP - Unbound Medicine ER -