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Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source.
Lab Chip. 2009 Mar 07; 9(5):733-6.LC

Abstract

A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (micro-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ren K
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
Liang Q
No affiliation info available
Mu X
No affiliation info available
Luo G
No affiliation info available
Wang Y
No affiliation info available

MeSH

AlgorithmsCapillary ElectrochromatographyEquipment DesignFluorescein-5-isothiocyanateFluorescent DyesLightMicrocomputersNanotechnology

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19224025

Citation

Ren, Kangning, et al. "Miniaturized High Throughput Detection System for Capillary Array Electrophoresis On Chip With Integrated Light Emitting Diode Array as Addressed Ring-shaped Light Source." Lab On a Chip, vol. 9, no. 5, 2009, pp. 733-6.
Ren K, Liang Q, Mu X, et al. Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source. Lab Chip. 2009;9(5):733-6.
Ren, K., Liang, Q., Mu, X., Luo, G., & Wang, Y. (2009). Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source. Lab On a Chip, 9(5), 733-6. https://doi.org/10.1039/b810705j
Ren K, et al. Miniaturized High Throughput Detection System for Capillary Array Electrophoresis On Chip With Integrated Light Emitting Diode Array as Addressed Ring-shaped Light Source. Lab Chip. 2009 Mar 7;9(5):733-6. PubMed PMID: 19224025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source. AU - Ren,Kangning, AU - Liang,Qionglin, AU - Mu,Xuan, AU - Luo,Guoan, AU - Wang,Yiming, Y1 - 2008/11/28/ PY - 2009/2/19/entrez PY - 2009/2/19/pubmed PY - 2009/4/14/medline SP - 733 EP - 6 JF - Lab on a chip JO - Lab Chip VL - 9 IS - 5 N2 - A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (micro-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol. SN - 1473-0197 UR - https://www.unboundmedicine.com/medline/citation/19224025/Miniaturized_high_throughput_detection_system_for_capillary_array_electrophoresis_on_chip_with_integrated_light_emitting_diode_array_as_addressed_ring_shaped_light_source_ L2 - https://doi.org/10.1039/b810705j DB - PRIME DP - Unbound Medicine ER -