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Amperometric phenol biosensor based on horseradish peroxidase entrapped PVF and PPy composite film coated GC electrode.
Appl Biochem Biotechnol. 2010 Mar; 160(3):856-67.AB

Abstract

Polyvinylferrocene (PVF) was used as a mediator for the fabrication of a horseradish peroxidase (HRP)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (PPy). Through an electropolymerization process, enzyme HRP was entrapped with PPy in a three-electrode system onto a glassy carbon electrode previously covered with PVF, resulting in a composite polymeric matrix. Steady-state amperometric measurements were performed at -200 mV vs. Ag/AgCl in aqueous phosphate buffer containing NaCl 0.1 M (pH 6.8) in the presence of hydrogen peroxide. The response of the HRP-modified PVF electrode was investigated for various phenol derivatives, which were 4-chlorophenol, phenol, catechol, hydroquinone, 2-aminophenol, pyrogallol, m-cresol, and 4-methoxyphenol. Analytical parameters for the fabricated PVF electrode were obtained from the calibration curves. The highest sensitivity was obtained from the calibration of 4-chlorophenol as 29.91 nA/microM. The lowest detection limit was found to be 0.22 microM (S/N = 3) for catechol, and the highest detection limit was found to be 0.79 microM (S/N = 3) for 4-methoxyphenol among the tested derivatives. The biosensor can reach 95% of steady-state current in about 5 min. The electrode is stable for 2 months at 4 degrees Celsius.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Topcu Sulak M
Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Turkey.
Erhan E
No affiliation info available
Keskinler B
No affiliation info available

MeSH

BiocatalysisBiosensing TechniquesCarbonElectrochemistryElectrodesEnzymes, ImmobilizedFerrous CompoundsGlassHorseradish PeroxidaseHydrogen-Ion ConcentrationMethylene ChloridePhenolPolymersPolyvinylsPyrrolesWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19224403

Citation

Topcu Sulak, Meral, et al. "Amperometric Phenol Biosensor Based On Horseradish Peroxidase Entrapped PVF and PPy Composite Film Coated GC Electrode." Applied Biochemistry and Biotechnology, vol. 160, no. 3, 2010, pp. 856-67.
Topcu Sulak M, Erhan E, Keskinler B. Amperometric phenol biosensor based on horseradish peroxidase entrapped PVF and PPy composite film coated GC electrode. Appl Biochem Biotechnol. 2010;160(3):856-67.
Topcu Sulak, M., Erhan, E., & Keskinler, B. (2010). Amperometric phenol biosensor based on horseradish peroxidase entrapped PVF and PPy composite film coated GC electrode. Applied Biochemistry and Biotechnology, 160(3), 856-67. https://doi.org/10.1007/s12010-009-8534-y
Topcu Sulak M, Erhan E, Keskinler B. Amperometric Phenol Biosensor Based On Horseradish Peroxidase Entrapped PVF and PPy Composite Film Coated GC Electrode. Appl Biochem Biotechnol. 2010;160(3):856-67. PubMed PMID: 19224403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric phenol biosensor based on horseradish peroxidase entrapped PVF and PPy composite film coated GC electrode. AU - Topcu Sulak,Meral, AU - Erhan,Elif, AU - Keskinler,Bülent, Y1 - 2009/02/18/ PY - 2008/10/28/received PY - 2009/01/20/accepted PY - 2009/2/19/entrez PY - 2009/2/19/pubmed PY - 2010/3/24/medline SP - 856 EP - 67 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 160 IS - 3 N2 - Polyvinylferrocene (PVF) was used as a mediator for the fabrication of a horseradish peroxidase (HRP)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (PPy). Through an electropolymerization process, enzyme HRP was entrapped with PPy in a three-electrode system onto a glassy carbon electrode previously covered with PVF, resulting in a composite polymeric matrix. Steady-state amperometric measurements were performed at -200 mV vs. Ag/AgCl in aqueous phosphate buffer containing NaCl 0.1 M (pH 6.8) in the presence of hydrogen peroxide. The response of the HRP-modified PVF electrode was investigated for various phenol derivatives, which were 4-chlorophenol, phenol, catechol, hydroquinone, 2-aminophenol, pyrogallol, m-cresol, and 4-methoxyphenol. Analytical parameters for the fabricated PVF electrode were obtained from the calibration curves. The highest sensitivity was obtained from the calibration of 4-chlorophenol as 29.91 nA/microM. The lowest detection limit was found to be 0.22 microM (S/N = 3) for catechol, and the highest detection limit was found to be 0.79 microM (S/N = 3) for 4-methoxyphenol among the tested derivatives. The biosensor can reach 95% of steady-state current in about 5 min. The electrode is stable for 2 months at 4 degrees Celsius. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/19224403/Amperometric_phenol_biosensor_based_on_horseradish_peroxidase_entrapped_PVF_and_PPy_composite_film_coated_GC_electrode_ L2 - https://dx.doi.org/10.1007/s12010-009-8534-y DB - PRIME DP - Unbound Medicine ER -