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Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2009 Mar; 23(6):877-84.RC

Abstract

Unambiguous identification of mycotoxin-producing fungal species as Fusarium is of great relevance to agriculture and the food-producing industry as well as in medicine. Protein profiles of intact fungal spores, such as Penicillium, Aspergillus and Trichoderma, derived from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were shown to provide a rapid and straightforward method for species identification and characterization. In this study, we applied this approach to five different Fusarium spp. strains which are known to affect the growth of different grain plants. To obtain a suitable MALDI matrix system and sample preparation method, thin-layer, dried-droplet and sandwich methods and several MALDI matrices, namely CHCA, DHB, FA, SA and THAP dissolved in various solvent mixtures (organic solvents such as ACN, MeOH, EtOH and iPrOH and for the aqueous phase water and 0.1% TFA), were evaluated in terms of mass spectrometric pattern and signal intensities. The most significant peptide/protein profiles were obtained with 10 mg ferulic acid (FA) in 1 mL ACN/0.1% TFA (7:3, v/v) used as matrix system. Mixing the spores with the matrix solution directly on the MALDI target (dried-droplet technique) resulted in an evenly distributed spores/matrix crystal layer, yielding highly reproducible peptide/protein profiles from the spore surfaces. Numerous abundant ions throughout the investigated m/z range (m/z 1500-15 000) could be detected. Differences in the obtained mass spectral patterns allowed the differentiation of spores of various Fusarium species.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kemptner J
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.
Marchetti-Deschmann M
No affiliation info available
Mach R
No affiliation info available
Druzhinina IS
No affiliation info available
Kubicek CP
No affiliation info available
Allmaier G
No affiliation info available

MeSH

Fungal ProteinsFusariumReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpores, Fungal

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19224532

Citation

Kemptner, Jasmin, et al. "Evaluation of Matrix-assisted Laser Desorption/ionization (MALDI) Preparation Techniques for Surface Characterization of Intact Fusarium Spores By MALDI Linear Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 6, 2009, pp. 877-84.
Kemptner J, Marchetti-Deschmann M, Mach R, et al. Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(6):877-84.
Kemptner, J., Marchetti-Deschmann, M., Mach, R., Druzhinina, I. S., Kubicek, C. P., & Allmaier, G. (2009). Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 23(6), 877-84. https://doi.org/10.1002/rcm.3949
Kemptner J, et al. Evaluation of Matrix-assisted Laser Desorption/ionization (MALDI) Preparation Techniques for Surface Characterization of Intact Fusarium Spores By MALDI Linear Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2009;23(6):877-84. PubMed PMID: 19224532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry. AU - Kemptner,Jasmin, AU - Marchetti-Deschmann,Martina, AU - Mach,Robert, AU - Druzhinina,Irina S, AU - Kubicek,Christian P, AU - Allmaier,Günter, PY - 2009/2/19/entrez PY - 2009/2/19/pubmed PY - 2009/4/23/medline SP - 877 EP - 84 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 6 N2 - Unambiguous identification of mycotoxin-producing fungal species as Fusarium is of great relevance to agriculture and the food-producing industry as well as in medicine. Protein profiles of intact fungal spores, such as Penicillium, Aspergillus and Trichoderma, derived from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were shown to provide a rapid and straightforward method for species identification and characterization. In this study, we applied this approach to five different Fusarium spp. strains which are known to affect the growth of different grain plants. To obtain a suitable MALDI matrix system and sample preparation method, thin-layer, dried-droplet and sandwich methods and several MALDI matrices, namely CHCA, DHB, FA, SA and THAP dissolved in various solvent mixtures (organic solvents such as ACN, MeOH, EtOH and iPrOH and for the aqueous phase water and 0.1% TFA), were evaluated in terms of mass spectrometric pattern and signal intensities. The most significant peptide/protein profiles were obtained with 10 mg ferulic acid (FA) in 1 mL ACN/0.1% TFA (7:3, v/v) used as matrix system. Mixing the spores with the matrix solution directly on the MALDI target (dried-droplet technique) resulted in an evenly distributed spores/matrix crystal layer, yielding highly reproducible peptide/protein profiles from the spore surfaces. Numerous abundant ions throughout the investigated m/z range (m/z 1500-15 000) could be detected. Differences in the obtained mass spectral patterns allowed the differentiation of spores of various Fusarium species. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/19224532/Evaluation_of_matrix_assisted_laser_desorption/ionization__MALDI__preparation_techniques_for_surface_characterization_of_intact_Fusarium_spores_by_MALDI_linear_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3949 DB - PRIME DP - Unbound Medicine ER -