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Ifenprodil induced antinociception and decreased the expression of NR2B subunits in the dorsal horn after chronic dorsal root ganglia compression in rats.
Anesth Analg. 2009 Mar; 108(3):1015-20.A&A

Abstract

BACKGROUND

Spinal N-methyl D-aspartate receptors play an important role in the pathogenesis of neuropathic pain, and administration of N-methyl D-aspartate receptor antagonists can attenuate this hyperpathia. Ifenprodil is an antagonist selective for N-methyl D-aspartate receptor 2B (NR2B) subunits. Several researches have reported effective analgesia of ifenprodil in animal models of neuropathic pain. We extended this work to include chronic compression of the dorsal root ganglia (CCD).

METHODS

The paw withdrawal mechanical threshold and paw withdrawal thermal latency tests were used to assess mechanical allodynia and thermal hyperalgesia after a CCD operation and intrathecal injection of ifenprodil. We used immunohistochemistry and immunoblotting to investigate the effect of ifenprodil on NR2B subunits expression in CCD rats.

RESULTS

The data revealed increased expression of NR2B subunits in the superficial dorsal horn in CCD rats. We found that, in addition to a marked suppression of thermal hyperalgesia and mechanical allodynia, intrathecal injection ifenprodil treatment causes a decreased expression of NR2B in the spinal cord.

CONCLUSIONS

These data suggest that ifenprodil induced antinociception in CCD rats and provided further evidence for the important role of NR2B subunits in the development of neuropathic pain.

Authors+Show Affiliations

Department of Anesthesiology, Drum Tower Hospital, Medical Department of Nanjing University, Nanjing 210008, Jiangsu Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19224818

Citation

Zhang, Wei, et al. "Ifenprodil Induced Antinociception and Decreased the Expression of NR2B Subunits in the Dorsal Horn After Chronic Dorsal Root Ganglia Compression in Rats." Anesthesia and Analgesia, vol. 108, no. 3, 2009, pp. 1015-20.
Zhang W, Shi CX, Gu XP, et al. Ifenprodil induced antinociception and decreased the expression of NR2B subunits in the dorsal horn after chronic dorsal root ganglia compression in rats. Anesth Analg. 2009;108(3):1015-20.
Zhang, W., Shi, C. X., Gu, X. P., Ma, Z. L., & Zhu, W. (2009). Ifenprodil induced antinociception and decreased the expression of NR2B subunits in the dorsal horn after chronic dorsal root ganglia compression in rats. Anesthesia and Analgesia, 108(3), 1015-20. https://doi.org/10.1213/ane.0b013e318193ffd2
Zhang W, et al. Ifenprodil Induced Antinociception and Decreased the Expression of NR2B Subunits in the Dorsal Horn After Chronic Dorsal Root Ganglia Compression in Rats. Anesth Analg. 2009;108(3):1015-20. PubMed PMID: 19224818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ifenprodil induced antinociception and decreased the expression of NR2B subunits in the dorsal horn after chronic dorsal root ganglia compression in rats. AU - Zhang,Wei, AU - Shi,Chang-Xi, AU - Gu,Xiao-ping, AU - Ma,Zheng-Liang, AU - Zhu,Wei, PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2009/3/13/medline SP - 1015 EP - 20 JF - Anesthesia and analgesia JO - Anesth Analg VL - 108 IS - 3 N2 - BACKGROUND: Spinal N-methyl D-aspartate receptors play an important role in the pathogenesis of neuropathic pain, and administration of N-methyl D-aspartate receptor antagonists can attenuate this hyperpathia. Ifenprodil is an antagonist selective for N-methyl D-aspartate receptor 2B (NR2B) subunits. Several researches have reported effective analgesia of ifenprodil in animal models of neuropathic pain. We extended this work to include chronic compression of the dorsal root ganglia (CCD). METHODS: The paw withdrawal mechanical threshold and paw withdrawal thermal latency tests were used to assess mechanical allodynia and thermal hyperalgesia after a CCD operation and intrathecal injection of ifenprodil. We used immunohistochemistry and immunoblotting to investigate the effect of ifenprodil on NR2B subunits expression in CCD rats. RESULTS: The data revealed increased expression of NR2B subunits in the superficial dorsal horn in CCD rats. We found that, in addition to a marked suppression of thermal hyperalgesia and mechanical allodynia, intrathecal injection ifenprodil treatment causes a decreased expression of NR2B in the spinal cord. CONCLUSIONS: These data suggest that ifenprodil induced antinociception in CCD rats and provided further evidence for the important role of NR2B subunits in the development of neuropathic pain. SN - 1526-7598 UR - https://www.unboundmedicine.com/medline/citation/19224818/Ifenprodil_induced_antinociception_and_decreased_the_expression_of_NR2B_subunits_in_the_dorsal_horn_after_chronic_dorsal_root_ganglia_compression_in_rats_ L2 - https://doi.org/10.1213/ane.0b013e318193ffd2 DB - PRIME DP - Unbound Medicine ER -