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Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury.
Br J Ophthalmol. 2009 Jul; 93(7):964-8.BJ

Abstract

AIM

To longitudinally investigate retinal ganglion cell (RGC) expression of Thy-1, a cell-surface glycoprotein specifically expressed in RGCs, with a blue-light confocal scanning laser ophthalmoscope, following retinal ischaemia induced by acute elevation of intraocular pressure.

METHODS

A blue-light confocal scanning laser ophthalmoscope (bCSLO, 460 nm excitation and 490 nm detection) was used to image Thy1-cyan fluorescent protein (CFP) mice before and weekly for 4 weeks after transiently elevating the intraocular pressure to 115 mm Hg for 45 min (n = 4) or 90 min (n = 5) to induce ischaemic injury. Corresponding retinal areas before and after the intraocular pressure (IOP) elevation, during the period of ischaemic reperfusion, were compared, and the fluorescent spots (Thy-1 expressing RGCs) were counted. The longitudinal profile of CFP-expressing RGCs was modelled with a linear regression equation. The spatial distribution of RGC damage was analysed in the superior, nasal, inferior and temporal quadrants of the retina.

RESULTS

No significant change was found at 4 weeks after 45 min of IOP elevation (n = 4, p = 0.465). The average RGC densities before and 4 weeks after IOP elevation were 1660 (SD 242) cells/mm2 and 1624 (209) cells/mm2, respectively. However, significant loss of CFP-expressing RGCs was detected at 1 week following 90 min of IOP elevation (n = 5, p<0.001). After this initial RGC loss, no significant change was detected subsequently. The proportion of RGC fluorescence remaining was variable and ranged from 14.5% to 79.5% at 4 weeks after the IOP elevation. The average RGC densities before and 4 weeks after IOP elevation were 1443 (162) cells/mm2 and 680 (385) cells/mm2, respectively. Diffuse loss of fluorescent RGCs was observed in the spatial distribution analysis.

CONCLUSIONS

The longitudinal profile of Thy-1 expressing RGC fluorescence loss after ischaemic injury is non-progressive and unrelated to the duration of reperfusion.

Authors+Show Affiliations

Hamilton Glaucoma Center, University of California, San Diego, La Jolla, CA 92093-0946, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19224902

Citation

Leung, C K S., et al. "Longitudinal Profile of Retinal Ganglion Cell Damage Assessed With Blue-light Confocal Scanning Laser Ophthalmoscopy After Ischaemic Reperfusion Injury." The British Journal of Ophthalmology, vol. 93, no. 7, 2009, pp. 964-8.
Leung CK, Lindsey JD, Chen L, et al. Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury. Br J Ophthalmol. 2009;93(7):964-8.
Leung, C. K., Lindsey, J. D., Chen, L., Liu, Q., & Weinreb, R. N. (2009). Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury. The British Journal of Ophthalmology, 93(7), 964-8. https://doi.org/10.1136/bjo.2008.150482
Leung CK, et al. Longitudinal Profile of Retinal Ganglion Cell Damage Assessed With Blue-light Confocal Scanning Laser Ophthalmoscopy After Ischaemic Reperfusion Injury. Br J Ophthalmol. 2009;93(7):964-8. PubMed PMID: 19224902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Longitudinal profile of retinal ganglion cell damage assessed with blue-light confocal scanning laser ophthalmoscopy after ischaemic reperfusion injury. AU - Leung,C K S, AU - Lindsey,J D, AU - Chen,L, AU - Liu,Q, AU - Weinreb,R N, Y1 - 2009/02/17/ PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2009/7/16/medline SP - 964 EP - 8 JF - The British journal of ophthalmology JO - Br J Ophthalmol VL - 93 IS - 7 N2 - AIM: To longitudinally investigate retinal ganglion cell (RGC) expression of Thy-1, a cell-surface glycoprotein specifically expressed in RGCs, with a blue-light confocal scanning laser ophthalmoscope, following retinal ischaemia induced by acute elevation of intraocular pressure. METHODS: A blue-light confocal scanning laser ophthalmoscope (bCSLO, 460 nm excitation and 490 nm detection) was used to image Thy1-cyan fluorescent protein (CFP) mice before and weekly for 4 weeks after transiently elevating the intraocular pressure to 115 mm Hg for 45 min (n = 4) or 90 min (n = 5) to induce ischaemic injury. Corresponding retinal areas before and after the intraocular pressure (IOP) elevation, during the period of ischaemic reperfusion, were compared, and the fluorescent spots (Thy-1 expressing RGCs) were counted. The longitudinal profile of CFP-expressing RGCs was modelled with a linear regression equation. The spatial distribution of RGC damage was analysed in the superior, nasal, inferior and temporal quadrants of the retina. RESULTS: No significant change was found at 4 weeks after 45 min of IOP elevation (n = 4, p = 0.465). The average RGC densities before and 4 weeks after IOP elevation were 1660 (SD 242) cells/mm2 and 1624 (209) cells/mm2, respectively. However, significant loss of CFP-expressing RGCs was detected at 1 week following 90 min of IOP elevation (n = 5, p<0.001). After this initial RGC loss, no significant change was detected subsequently. The proportion of RGC fluorescence remaining was variable and ranged from 14.5% to 79.5% at 4 weeks after the IOP elevation. The average RGC densities before and 4 weeks after IOP elevation were 1443 (162) cells/mm2 and 680 (385) cells/mm2, respectively. Diffuse loss of fluorescent RGCs was observed in the spatial distribution analysis. CONCLUSIONS: The longitudinal profile of Thy-1 expressing RGC fluorescence loss after ischaemic injury is non-progressive and unrelated to the duration of reperfusion. SN - 1468-2079 UR - https://www.unboundmedicine.com/medline/citation/19224902/Longitudinal_profile_of_retinal_ganglion_cell_damage_assessed_with_blue_light_confocal_scanning_laser_ophthalmoscopy_after_ischaemic_reperfusion_injury_ L2 - https://bjo.bmj.com/lookup/pmidlookup?view=long&amp;pmid=19224902 DB - PRIME DP - Unbound Medicine ER -