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Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar.
J Antimicrob Chemother. 2009 Apr; 63(4):699-703.JA

Abstract

OBJECTIVES

Cefoxitin is today the substance of choice for the phenotypic detection of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the influence of incubation temperature in the standard range, i.e. 35-37 degrees C, and time, i.e. 18-20 h, versus a full 24 h.

METHODS

Cefoxitin disc testing was examined at incubation temperatures of 35 and 36 degrees C and times of 18-20 and 24 h, respectively, for 94 mecA-negative and 49 mecA-positive S. aureus on Iso-Sensitest agar using a semi-confluent inoculum.

RESULTS

Cefoxitin inhibition zones on Iso-Sensitest agar were larger at temperatures above 35 degrees C; two isolates (4%, 95% confidence interval=0.5-14%) incubated at 36 degrees C were falsely categorized as susceptible to methicillin. Incubation time across 18-24 h did not impact results.

CONCLUSIONS

Detection of methicillin resistance in S. aureus using the cefoxitin disc method with a semi-confluent inoculum on Iso-Sensitest agar is influenced by incubation temperature, and the temperature should not exceed 35 degrees C for the reliable detection of MRSA.

Authors+Show Affiliations

National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark. rsk@ssi.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19224945

Citation

Skov, R, et al. "Effects of Temperature On the Detection of Methicillin Resistance in Staphylococcus Aureus Using Cefoxitin Disc Diffusion Testing With Iso-Sensitest Agar." The Journal of Antimicrobial Chemotherapy, vol. 63, no. 4, 2009, pp. 699-703.
Skov R, Smyth R, Yusof A, et al. Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar. J Antimicrob Chemother. 2009;63(4):699-703.
Skov, R., Smyth, R., Yusof, A., Karlsson, A., Mills, K., Frimodt-Moller, N., & Kahlmeter, G. (2009). Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar. The Journal of Antimicrobial Chemotherapy, 63(4), 699-703. https://doi.org/10.1093/jac/dkp019
Skov R, et al. Effects of Temperature On the Detection of Methicillin Resistance in Staphylococcus Aureus Using Cefoxitin Disc Diffusion Testing With Iso-Sensitest Agar. J Antimicrob Chemother. 2009;63(4):699-703. PubMed PMID: 19224945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar. AU - Skov,R, AU - Smyth,R, AU - Yusof,A, AU - Karlsson,A, AU - Mills,K, AU - Frimodt-Moller,N, AU - Kahlmeter,G, Y1 - 2009/02/18/ PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2009/4/25/medline SP - 699 EP - 703 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 63 IS - 4 N2 - OBJECTIVES: Cefoxitin is today the substance of choice for the phenotypic detection of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the influence of incubation temperature in the standard range, i.e. 35-37 degrees C, and time, i.e. 18-20 h, versus a full 24 h. METHODS: Cefoxitin disc testing was examined at incubation temperatures of 35 and 36 degrees C and times of 18-20 and 24 h, respectively, for 94 mecA-negative and 49 mecA-positive S. aureus on Iso-Sensitest agar using a semi-confluent inoculum. RESULTS: Cefoxitin inhibition zones on Iso-Sensitest agar were larger at temperatures above 35 degrees C; two isolates (4%, 95% confidence interval=0.5-14%) incubated at 36 degrees C were falsely categorized as susceptible to methicillin. Incubation time across 18-24 h did not impact results. CONCLUSIONS: Detection of methicillin resistance in S. aureus using the cefoxitin disc method with a semi-confluent inoculum on Iso-Sensitest agar is influenced by incubation temperature, and the temperature should not exceed 35 degrees C for the reliable detection of MRSA. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/19224945/Effects_of_temperature_on_the_detection_of_methicillin_resistance_in_Staphylococcus_aureus_using_cefoxitin_disc_diffusion_testing_with_Iso_Sensitest_agar_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp019 DB - PRIME DP - Unbound Medicine ER -