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Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis.
Scand Cardiovasc J. 2009 Apr; 43(2):136-44.SC

Abstract

OBJECTIVES

Interferon gamma (IFN-gamma) synthesis in peripheral blood mononuclear cells (PBMCs) is suppressed after major surgical trauma. Interleukin-12 (IL-12) has been shown to stimulate IFN-gamma-synthesis. We hypothesised that exogenous IL-12 can increase perioperative pro-inflammatory cytokine release. We therefore assessed the effect of IL-12 on IFN-gamma-synthesis and pro-inflammatory cytokine release in vitro before and after cardiac surgery.

DESIGN

In this prospective study, PBMCs from 20 elective cardiac surgery patients were stimulated for 24 hours with staphylococcal enterotoxin B and lipopolysaccharide before surgery (d0) and on the 1st (d1), 3rd (d3) and 5th (d5) postoperative days. IL-12 was added at each time point investigated. IFN-gamma, IL-6, tumour necrosis factor-alpha (TNF-alpha), IL-2, IL-4, IL-5, and IL-10 concentrations were assayed.

RESULTS

IFN-gamma-synthesis was significantly reduced at d1, d3 and d5. When IL-12 was added, IFN-gamma-synthesis returned to preoperative levels at d1, d3 and d5. Neither IL-6 nor TNF-alpha-synthesis was influenced by IL-12.

CONCLUSIONS

IFN-gamma synthesis is significantly reduced after major surgical trauma. IL-12 increases IFN-gamma-synthesis before and after surgery without influencing pro-inflammatory cytokine synthesis.

Authors+Show Affiliations

Department of Cardiovascular Surgery, German Armed Forces Central Hospital, Koblenz, Germany. dr.axel.franke@t-online.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19225954

Citation

Franke, Axel, et al. "Postoperative PMBC-derived IL-6 and TNF-alpha-release Is Uninfluenced By IL-12-mediated Restoration of IFN-gamma Synthesis." Scandinavian Cardiovascular Journal : SCJ, vol. 43, no. 2, 2009, pp. 136-44.
Franke A, Lante W, Kurig E, et al. Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis. Scand Cardiovasc J. 2009;43(2):136-44.
Franke, A., Lante, W., Kurig, E., Zoeller, L. G., Kollig, E., & Markewitz, A. (2009). Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis. Scandinavian Cardiovascular Journal : SCJ, 43(2), 136-44. https://doi.org/10.1080/14017430802409699
Franke A, et al. Postoperative PMBC-derived IL-6 and TNF-alpha-release Is Uninfluenced By IL-12-mediated Restoration of IFN-gamma Synthesis. Scand Cardiovasc J. 2009;43(2):136-44. PubMed PMID: 19225954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postoperative PMBC-derived IL-6 and TNF-alpha-release is uninfluenced by IL-12-mediated restoration of IFN-gamma synthesis. AU - Franke,Axel, AU - Lante,Wolfgang, AU - Kurig,Edmond, AU - Zoeller,Lothar G, AU - Kollig,Erwin, AU - Markewitz,Andreas, PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2009/5/9/medline SP - 136 EP - 44 JF - Scandinavian cardiovascular journal : SCJ JO - Scand Cardiovasc J VL - 43 IS - 2 N2 - OBJECTIVES: Interferon gamma (IFN-gamma) synthesis in peripheral blood mononuclear cells (PBMCs) is suppressed after major surgical trauma. Interleukin-12 (IL-12) has been shown to stimulate IFN-gamma-synthesis. We hypothesised that exogenous IL-12 can increase perioperative pro-inflammatory cytokine release. We therefore assessed the effect of IL-12 on IFN-gamma-synthesis and pro-inflammatory cytokine release in vitro before and after cardiac surgery. DESIGN: In this prospective study, PBMCs from 20 elective cardiac surgery patients were stimulated for 24 hours with staphylococcal enterotoxin B and lipopolysaccharide before surgery (d0) and on the 1st (d1), 3rd (d3) and 5th (d5) postoperative days. IL-12 was added at each time point investigated. IFN-gamma, IL-6, tumour necrosis factor-alpha (TNF-alpha), IL-2, IL-4, IL-5, and IL-10 concentrations were assayed. RESULTS: IFN-gamma-synthesis was significantly reduced at d1, d3 and d5. When IL-12 was added, IFN-gamma-synthesis returned to preoperative levels at d1, d3 and d5. Neither IL-6 nor TNF-alpha-synthesis was influenced by IL-12. CONCLUSIONS: IFN-gamma synthesis is significantly reduced after major surgical trauma. IL-12 increases IFN-gamma-synthesis before and after surgery without influencing pro-inflammatory cytokine synthesis. SN - 1651-2006 UR - https://www.unboundmedicine.com/medline/citation/19225954/Postoperative_PMBC_derived_IL_6_and_TNF_alpha_release_is_uninfluenced_by_IL_12_mediated_restoration_of_IFN_gamma_synthesis_ L2 - https://www.tandfonline.com/doi/full/10.1080/14017430802409699 DB - PRIME DP - Unbound Medicine ER -