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Multiple introductions of the Spiroplasma bacterial endosymbiont into Drosophila.
Mol Ecol. 2009 Mar; 18(6):1294-305.ME

Abstract

Bacterial endosymbionts are common in insects and can have dramatic effects on their host's evolution. So far, the only heritable symbionts found in Drosophila have been Wolbachia and Spiroplasma. While the incidence and effects of Wolbachia have been studied extensively, the prevalence and significance of Spiroplasma infections in Drosophila are less clear. These small, gram-positive, helical bacteria infect a diverse array of plant and arthropod hosts, conferring a variety of fitness effects. Male-killing Spiroplasma are known from certain Drosophila species; however, in others, Spiroplasma appear not to affect sex ratio. Previous studies have identified different Spiroplasma haplotypes in Drosophila populations, although no extensive surveys have yet been reported. We used a multilocus sequence analysis to reconstruct a robust Spiroplasma endosymbiont phylogeny, assess genetic diversity, and look for evidence of recombination. Six loci were sequenced from over 65 Spiroplasma-infected individuals from nine different Drosophila species. Analysis of these sequences reveals at least five separate introductions of four phylogenetically distinct Spiroplasma haplotypes, indicating that more extensive sampling will likely reveal an even greater Spiroplasma endosymbiont diversity. Patterns of variation in Drosophila mitochondrial haplotypes in Spiroplasma-infected and uninfected flies imply imperfect vertical transmission in host populations and possible horizontal transmission.

Authors+Show Affiliations

Department of Ecology and Evolutionary Biology Biosciences, University of Arizona, Tucson, 85721-0088, USA. thaselko@ucsd.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19226322

Citation

Haselkorn, Tamara S., et al. "Multiple Introductions of the Spiroplasma Bacterial Endosymbiont Into Drosophila." Molecular Ecology, vol. 18, no. 6, 2009, pp. 1294-305.
Haselkorn TS, Markow TA, Moran NA. Multiple introductions of the Spiroplasma bacterial endosymbiont into Drosophila. Mol Ecol. 2009;18(6):1294-305.
Haselkorn, T. S., Markow, T. A., & Moran, N. A. (2009). Multiple introductions of the Spiroplasma bacterial endosymbiont into Drosophila. Molecular Ecology, 18(6), 1294-305. https://doi.org/10.1111/j.1365-294X.2009.04085.x
Haselkorn TS, Markow TA, Moran NA. Multiple Introductions of the Spiroplasma Bacterial Endosymbiont Into Drosophila. Mol Ecol. 2009;18(6):1294-305. PubMed PMID: 19226322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple introductions of the Spiroplasma bacterial endosymbiont into Drosophila. AU - Haselkorn,Tamara S, AU - Markow,Therese A, AU - Moran,Nancy A, Y1 - 2009/02/17/ PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2009/3/25/medline SP - 1294 EP - 305 JF - Molecular ecology JO - Mol. Ecol. VL - 18 IS - 6 N2 - Bacterial endosymbionts are common in insects and can have dramatic effects on their host's evolution. So far, the only heritable symbionts found in Drosophila have been Wolbachia and Spiroplasma. While the incidence and effects of Wolbachia have been studied extensively, the prevalence and significance of Spiroplasma infections in Drosophila are less clear. These small, gram-positive, helical bacteria infect a diverse array of plant and arthropod hosts, conferring a variety of fitness effects. Male-killing Spiroplasma are known from certain Drosophila species; however, in others, Spiroplasma appear not to affect sex ratio. Previous studies have identified different Spiroplasma haplotypes in Drosophila populations, although no extensive surveys have yet been reported. We used a multilocus sequence analysis to reconstruct a robust Spiroplasma endosymbiont phylogeny, assess genetic diversity, and look for evidence of recombination. Six loci were sequenced from over 65 Spiroplasma-infected individuals from nine different Drosophila species. Analysis of these sequences reveals at least five separate introductions of four phylogenetically distinct Spiroplasma haplotypes, indicating that more extensive sampling will likely reveal an even greater Spiroplasma endosymbiont diversity. Patterns of variation in Drosophila mitochondrial haplotypes in Spiroplasma-infected and uninfected flies imply imperfect vertical transmission in host populations and possible horizontal transmission. SN - 1365-294X UR - https://www.unboundmedicine.com/medline/citation/19226322/Multiple_introductions_of_the_Spiroplasma_bacterial_endosymbiont_into_Drosophila_ L2 - https://doi.org/10.1111/j.1365-294X.2009.04085.x DB - PRIME DP - Unbound Medicine ER -