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Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains.
J Appl Microbiol 2009; 106(5):1618-28JA

Abstract

AIMS

Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium.

METHODS AND RESULTS

The tetrasaccharide consists of three rhamnose residues and an unique monosaccharide--anthrose. As anthrose was not found in spores of related strains of bacteria, we envisioned the detection of B. anthracis spores based on antibodies against anthrose-containing polysaccharides. Carbohydrate-protein conjugates containing the synthetic tetrasaccharide, an anthrose-rhamnose disaccharide or anthrose alone were employed to immunize mice. All three formulations were immunogenic and elicited IgG responses with different fine specificities. All sera and monoclonal antibodies derived from tetrasaccharide immunized mice cross-reacted not only with spore lysates of a panel of virulent B. anthracis strains, but also with some of the B. cereus strains tested.

CONCLUSIONS

Our results demonstrate that antibodies to synthetic carbohydrates are useful tools for epitope analyses of complex carbohydrate antigens and for the detection of particular target structures in biological specimens.

SIGNIFICANCE AND IMPACT OF THE STUDY

Although not strictly specific for B. anthracis spores, antibodies against the tetrasaccharide may have potential as immuno-capturing components for a highly sensitive spore detection system.

Authors+Show Affiliations

Molecular Immunology, Swiss Tropical Institute, Basel, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19226390

Citation

Tamborrini, M, et al. "Immuno-detection of Anthrose Containing Tetrasaccharide in the Exosporium of Bacillus Anthracis and Bacillus Cereus Strains." Journal of Applied Microbiology, vol. 106, no. 5, 2009, pp. 1618-28.
Tamborrini M, Oberli MA, Werz DB, et al. Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains. J Appl Microbiol. 2009;106(5):1618-28.
Tamborrini, M., Oberli, M. A., Werz, D. B., Schürch, N., Frey, J., Seeberger, P. H., & Pluschke, G. (2009). Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains. Journal of Applied Microbiology, 106(5), pp. 1618-28. doi:10.1111/j.1365-2672.2008.04129.x.
Tamborrini M, et al. Immuno-detection of Anthrose Containing Tetrasaccharide in the Exosporium of Bacillus Anthracis and Bacillus Cereus Strains. J Appl Microbiol. 2009;106(5):1618-28. PubMed PMID: 19226390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains. AU - Tamborrini,M, AU - Oberli,M A, AU - Werz,D B, AU - Schürch,N, AU - Frey,J, AU - Seeberger,P H, AU - Pluschke,G, Y1 - 2009/02/16/ PY - 2009/2/20/entrez PY - 2009/2/20/pubmed PY - 2010/4/17/medline SP - 1618 EP - 28 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 106 IS - 5 N2 - AIMS: Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium. METHODS AND RESULTS: The tetrasaccharide consists of three rhamnose residues and an unique monosaccharide--anthrose. As anthrose was not found in spores of related strains of bacteria, we envisioned the detection of B. anthracis spores based on antibodies against anthrose-containing polysaccharides. Carbohydrate-protein conjugates containing the synthetic tetrasaccharide, an anthrose-rhamnose disaccharide or anthrose alone were employed to immunize mice. All three formulations were immunogenic and elicited IgG responses with different fine specificities. All sera and monoclonal antibodies derived from tetrasaccharide immunized mice cross-reacted not only with spore lysates of a panel of virulent B. anthracis strains, but also with some of the B. cereus strains tested. CONCLUSIONS: Our results demonstrate that antibodies to synthetic carbohydrates are useful tools for epitope analyses of complex carbohydrate antigens and for the detection of particular target structures in biological specimens. SIGNIFICANCE AND IMPACT OF THE STUDY: Although not strictly specific for B. anthracis spores, antibodies against the tetrasaccharide may have potential as immuno-capturing components for a highly sensitive spore detection system. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/19226390/Immuno_detection_of_anthrose_containing_tetrasaccharide_in_the_exosporium_of_Bacillus_anthracis_and_Bacillus_cereus_strains_ L2 - https://doi.org/10.1111/j.1365-2672.2008.04129.x DB - PRIME DP - Unbound Medicine ER -