Extraction of two active polysaccharides from the yeast cell wall.Z Naturforsch C J Biosci. 2008 Nov-Dec; 63(11-12):919-21.ZN
Yeast cell wall matrix particles are composed entirely of beta-glucan and mannoprotein. Alkali-insoluble (1-->3)-beta-D-glucan was extracted from the yeast cell wall by an alkaline-acid method. IR spectra analysis showed that the product was chemically pure glucan, that is to say, it contained no other carbohydrates and proteins. So, the alkaline-acid method was ideal for extracting (1-->3)-beta-D-glucan from the yeast cell wall. We also purified and analyzed mannan oligosaccharides by the dilute alkali-Sevage method from the yeast cell wall.