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Local action of endogenous renal tubular atrial natriuretic peptide.
J Cell Physiol. 2009 Jun; 219(3):776-86.JC

Abstract

Up-regulation of atrial natriuretic peptide (ANP) mRNA in the kidneys in several disorders has been demonstrated; however, evidence that ANP synthesized by the kidney exerts a local function has never been produced. Therefore, we investigated whether endogenous ANP could modulate high glucose-stimulated TGF-beta1, collagen type I and nuclear factor-kappaB (NF-kappaB) in NRK-52E cells using transfection of ANP and ANP small interfering RNA (siANP). NRK-52E cells were grown with or without transfection with ANP plasmid; cells were also transfected with ANP siRNA or control siRNA. These cells were then stimulated with a high glucose concentration to modulate ANP, TGF-beta1, collagen type I, NF-kappaB and IkappaB-alpha, and the results showed that ANP, TGF-beta1, collagen type I and NF-kappaB significantly increased in untransfected cells, and the transfection of ANP significantly attenuated high glucose-activated TGF-beta1, collagen I and NF-kappaB expression. ANP siRNA knocked-down ANP but significantly increased TGF-beta1 and collagen I under normal glucose conditions; ANP siRNA decreased IkappaB-alpha but strongly enhanced high glucose-activated TGF-beta1, collagen type I and NF-kappaB. In contrast, medium from ANP-transfected cells attenuated high glucose-activated TGF-beta1 and collagen type I expression in NRK-52E cells transfected with siANP. In conclusion, our results demonstrated that siANP increased activation of TGF-beta1, collagen type I and NF-kappaB in NRK-52E cells under high glucose conditions, and medium from ANP-transfected cells attenuated high glucose-activated TGF-beta1 and collagen type I. This is the first study to demonstrate the auto/paracrine action of endogenous ANP in renal tubular cells on the attenuation of hyperglycemia-activated TGF-beta1 and NF-kappaB expression. J. Cell. Physiol. 219: 776-786, 2009. (c) 2009 Wiley-Liss, Inc.

Authors+Show Affiliations

Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19229880

Citation

Lo, Chao-Sheng, et al. "Local Action of Endogenous Renal Tubular Atrial Natriuretic Peptide." Journal of Cellular Physiology, vol. 219, no. 3, 2009, pp. 776-86.
Lo CS, Chen CH, Hsieh TJ, et al. Local action of endogenous renal tubular atrial natriuretic peptide. J Cell Physiol. 2009;219(3):776-86.
Lo, C. S., Chen, C. H., Hsieh, T. J., Lin, K. D., Hsiao, P. J., & Shin, S. J. (2009). Local action of endogenous renal tubular atrial natriuretic peptide. Journal of Cellular Physiology, 219(3), 776-86. https://doi.org/10.1002/jcp.21728
Lo CS, et al. Local Action of Endogenous Renal Tubular Atrial Natriuretic Peptide. J Cell Physiol. 2009;219(3):776-86. PubMed PMID: 19229880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Local action of endogenous renal tubular atrial natriuretic peptide. AU - Lo,Chao-Sheng, AU - Chen,Chao-Hung, AU - Hsieh,Tusty-Jiuan, AU - Lin,Kun-Der, AU - Hsiao,Pi-Jung, AU - Shin,Shyi-Jang, PY - 2009/2/21/entrez PY - 2009/2/21/pubmed PY - 2009/4/17/medline SP - 776 EP - 86 JF - Journal of cellular physiology JO - J Cell Physiol VL - 219 IS - 3 N2 - Up-regulation of atrial natriuretic peptide (ANP) mRNA in the kidneys in several disorders has been demonstrated; however, evidence that ANP synthesized by the kidney exerts a local function has never been produced. Therefore, we investigated whether endogenous ANP could modulate high glucose-stimulated TGF-beta1, collagen type I and nuclear factor-kappaB (NF-kappaB) in NRK-52E cells using transfection of ANP and ANP small interfering RNA (siANP). NRK-52E cells were grown with or without transfection with ANP plasmid; cells were also transfected with ANP siRNA or control siRNA. These cells were then stimulated with a high glucose concentration to modulate ANP, TGF-beta1, collagen type I, NF-kappaB and IkappaB-alpha, and the results showed that ANP, TGF-beta1, collagen type I and NF-kappaB significantly increased in untransfected cells, and the transfection of ANP significantly attenuated high glucose-activated TGF-beta1, collagen I and NF-kappaB expression. ANP siRNA knocked-down ANP but significantly increased TGF-beta1 and collagen I under normal glucose conditions; ANP siRNA decreased IkappaB-alpha but strongly enhanced high glucose-activated TGF-beta1, collagen type I and NF-kappaB. In contrast, medium from ANP-transfected cells attenuated high glucose-activated TGF-beta1 and collagen type I expression in NRK-52E cells transfected with siANP. In conclusion, our results demonstrated that siANP increased activation of TGF-beta1, collagen type I and NF-kappaB in NRK-52E cells under high glucose conditions, and medium from ANP-transfected cells attenuated high glucose-activated TGF-beta1 and collagen type I. This is the first study to demonstrate the auto/paracrine action of endogenous ANP in renal tubular cells on the attenuation of hyperglycemia-activated TGF-beta1 and NF-kappaB expression. J. Cell. Physiol. 219: 776-786, 2009. (c) 2009 Wiley-Liss, Inc. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/19229880/Local_action_of_endogenous_renal_tubular_atrial_natriuretic_peptide_ L2 - https://doi.org/10.1002/jcp.21728 DB - PRIME DP - Unbound Medicine ER -