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Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-kappaB pathway.

Abstract

Excessive production of inflammatory mediators such as nitric oxide (NO), prostaglandin E(2) (PGE2), and proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) from activated microglia contributes to uncontrolled inflammation in neurodegenerative diseases. It seems possible that treatment with anti-inflammatory agents, including plants used in Oriental medicine, might delay the progression of neurodegeneration through the inhibition of microglial activation. The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiber officinale Roscoe (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, PGE(2), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 cells, a mouse microglial cell line. GHE significantly inhibited the excessive production of NO, PGE(2), TNF-alpha, and IL-1beta in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. The molecular mechanisms that underlie GHE-mediated attenuation are related to the inhibition of the phosphorylation of three mitogen-activated protein kinases (MAPKs), extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38 MAPK, and c-Jun N-terminal kinase (JNK), and the activation of nuclear factor-kappaB (NF-kappaB). Our results indicate that GHE exhibits anti-inflammatory properties by suppressing the transcription of inflammatory mediator genes through the MAPK and NF-kappaB signaling pathways. The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases.

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  • Authors+Show Affiliations

    ,

    Department of Herbology, College of Oriental Medicine, Dongguk University, Seok-Jang Dong, Gyeongju 780-714, Republic of Korea.

    , , ,

    Source

    MeSH

    Animals
    Blotting, Western
    Cell Line
    Cell Survival
    Cytokines
    Dinoprostone
    Ginger
    Hexanes
    Inflammation
    Lipopolysaccharides
    Mice
    Microglia
    Mitogen-Activated Protein Kinases
    NF-kappa B
    Nitric Oxide
    Phosphorylation
    Reverse Transcriptase Polymerase Chain Reaction
    Solvents

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19233241

    Citation

    Jung, Hyo Won, et al. "Hexane Fraction of Zingiberis Rhizoma Crudus Extract Inhibits the Production of Nitric Oxide and Proinflammatory Cytokines in LPS-stimulated BV2 Microglial Cells Via the NF-kappaB Pathway." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 6, 2009, pp. 1190-7.
    Jung HW, Yoon CH, Park KM, et al. Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-kappaB pathway. Food Chem Toxicol. 2009;47(6):1190-7.
    Jung, H. W., Yoon, C. H., Park, K. M., Han, H. S., & Park, Y. K. (2009). Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-kappaB pathway. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(6), pp. 1190-7. doi:10.1016/j.fct.2009.02.012.
    Jung HW, et al. Hexane Fraction of Zingiberis Rhizoma Crudus Extract Inhibits the Production of Nitric Oxide and Proinflammatory Cytokines in LPS-stimulated BV2 Microglial Cells Via the NF-kappaB Pathway. Food Chem Toxicol. 2009;47(6):1190-7. PubMed PMID: 19233241.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-kappaB pathway. AU - Jung,Hyo Won, AU - Yoon,Cheol-Ho, AU - Park,Kwon Moo, AU - Han,Hyung Soo, AU - Park,Yong-Ki, Y1 - 2009/02/20/ PY - 2008/01/29/received PY - 2009/01/20/revised PY - 2009/02/09/accepted PY - 2009/2/24/entrez PY - 2009/2/24/pubmed PY - 2009/7/17/medline SP - 1190 EP - 7 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 47 IS - 6 N2 - Excessive production of inflammatory mediators such as nitric oxide (NO), prostaglandin E(2) (PGE2), and proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) from activated microglia contributes to uncontrolled inflammation in neurodegenerative diseases. It seems possible that treatment with anti-inflammatory agents, including plants used in Oriental medicine, might delay the progression of neurodegeneration through the inhibition of microglial activation. The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiber officinale Roscoe (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, PGE(2), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 cells, a mouse microglial cell line. GHE significantly inhibited the excessive production of NO, PGE(2), TNF-alpha, and IL-1beta in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. The molecular mechanisms that underlie GHE-mediated attenuation are related to the inhibition of the phosphorylation of three mitogen-activated protein kinases (MAPKs), extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38 MAPK, and c-Jun N-terminal kinase (JNK), and the activation of nuclear factor-kappaB (NF-kappaB). Our results indicate that GHE exhibits anti-inflammatory properties by suppressing the transcription of inflammatory mediator genes through the MAPK and NF-kappaB signaling pathways. The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19233241/Hexane_fraction_of_Zingiberis_Rhizoma_Crudus_extract_inhibits_the_production_of_nitric_oxide_and_proinflammatory_cytokines_in_LPS_stimulated_BV2_microglial_cells_via_the_NF_kappaB_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00077-5 DB - PRIME DP - Unbound Medicine ER -