Tags

Type your tag names separated by a space and hit enter

Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala).
Food Chem Toxicol. 2009 Jun; 47(6):1209-20.FC

Abstract

Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV-DAD/MS(n)-ESI and HPLC-UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentration-dependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation-contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale's phenolic compounds. Moreover, the extract's bioactivities suggest that they may constitute an interesting source of bioactive compounds whose complex chemical structures preclude either synthesis or isolation.

Authors+Show Affiliations

Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19233244

Citation

Ferreres, Federico, et al. "Metabolic Profiling and Biological Capacity of Pieris Brassicae Fed With Kale (Brassica Oleracea L. Var. Acephala)." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 6, 2009, pp. 1209-20.
Ferreres F, Fernandes F, Oliveira JM, et al. Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). Food Chem Toxicol. 2009;47(6):1209-20.
Ferreres, F., Fernandes, F., Oliveira, J. M., Valentão, P., Pereira, J. A., & Andrade, P. B. (2009). Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(6), 1209-20. https://doi.org/10.1016/j.fct.2009.02.014
Ferreres F, et al. Metabolic Profiling and Biological Capacity of Pieris Brassicae Fed With Kale (Brassica Oleracea L. Var. Acephala). Food Chem Toxicol. 2009;47(6):1209-20. PubMed PMID: 19233244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala). AU - Ferreres,Federico, AU - Fernandes,Fátima, AU - Oliveira,Jorge M A, AU - Valentão,Patrícia, AU - Pereira,José A, AU - Andrade,Paula B, Y1 - 2009/02/20/ PY - 2008/11/13/received PY - 2009/01/28/revised PY - 2009/02/10/accepted PY - 2009/2/24/entrez PY - 2009/2/24/pubmed PY - 2009/7/17/medline SP - 1209 EP - 20 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 47 IS - 6 N2 - Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV-DAD/MS(n)-ESI and HPLC-UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentration-dependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation-contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale's phenolic compounds. Moreover, the extract's bioactivities suggest that they may constitute an interesting source of bioactive compounds whose complex chemical structures preclude either synthesis or isolation. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19233244/Metabolic_profiling_and_biological_capacity_of_Pieris_brassicae_fed_with_kale__Brassica_oleracea_L__var__acephala__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00086-6 DB - PRIME DP - Unbound Medicine ER -