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The role of C/EBPdelta in the early stages of adipogenesis.
Biochimie. 2009 May; 91(5):654-7.B

Abstract

Adipocyte differentiation is a complex process triggered and facilitated by transcription factors such as peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein (C/EBP) alpha. Most about the cascade underlying the differentiation process, especially events in the early stages, remain to be elucidated. Early on in adipocyte differentiation, the C/EBPbeta and C/EBPdelta genes are rapidly induced to express and later activate PPARgamma and C/EBPalpha expression. C/EBPbeta also plays a crucial role in mitotic clonal expansion (MCE), the approximately two rounds of mitosis which occurs soon after preadipocytes are stimulated by differentiation inducers and a necessary step for adipocyte differentiation. However, the effect of C/EBPdelta, another member of the C/EBP family, on MCE remains unclear. In the present study, we investigated the role of C/EBPdelta in the early stages of adipogenesis. A remarkable induction of C/EBPdelta gene expression after the initiation of differentiation was observed not in proliferating preadipocytes, but in growth-arrested, differentiable cells. RNAi-mediated knockdown of C/EBPdelta dramatically suppressed cell growth after differentiation was induced, and inhibited conversion into lipid-laden adipocytes. Furthermore, silencing of C/EBPdelta impaired the expression of factor for adipocyte differentiation (fad) 49, which is up-regulated and plays a crucial role early in adipogenesis. Taken together, these findings show that C/EBPdelta is involved in MCE and gene expression in the early stages of adipocyte differentiation.

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Authors+Show Affiliations

Hishida T
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
Nishizuka M
No affiliation info available
Osada S
No affiliation info available
Imagawa M
No affiliation info available

MeSH

3T3-L1 CellsAdipogenesisAnimalsCCAAT-Enhancer-Binding Protein-deltaCell DifferentiationGene Expression Regulation, DevelopmentalMiceMitosisPhospholipid Transfer ProteinsRNA InterferenceReverse Transcriptase Polymerase Chain Reaction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19233245

Citation

Hishida, Tomoaki, et al. "The Role of C/EBPdelta in the Early Stages of Adipogenesis." Biochimie, vol. 91, no. 5, 2009, pp. 654-7.
Hishida T, Nishizuka M, Osada S, et al. The role of C/EBPdelta in the early stages of adipogenesis. Biochimie. 2009;91(5):654-7.
Hishida, T., Nishizuka, M., Osada, S., & Imagawa, M. (2009). The role of C/EBPdelta in the early stages of adipogenesis. Biochimie, 91(5), 654-7. https://doi.org/10.1016/j.biochi.2009.02.002
Hishida T, et al. The Role of C/EBPdelta in the Early Stages of Adipogenesis. Biochimie. 2009;91(5):654-7. PubMed PMID: 19233245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of C/EBPdelta in the early stages of adipogenesis. AU - Hishida,Tomoaki, AU - Nishizuka,Makoto, AU - Osada,Shigehiro, AU - Imagawa,Masayoshi, Y1 - 2009/02/20/ PY - 2008/11/12/received PY - 2009/02/10/accepted PY - 2009/2/24/entrez PY - 2009/2/24/pubmed PY - 2009/6/26/medline SP - 654 EP - 7 JF - Biochimie JO - Biochimie VL - 91 IS - 5 N2 - Adipocyte differentiation is a complex process triggered and facilitated by transcription factors such as peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein (C/EBP) alpha. Most about the cascade underlying the differentiation process, especially events in the early stages, remain to be elucidated. Early on in adipocyte differentiation, the C/EBPbeta and C/EBPdelta genes are rapidly induced to express and later activate PPARgamma and C/EBPalpha expression. C/EBPbeta also plays a crucial role in mitotic clonal expansion (MCE), the approximately two rounds of mitosis which occurs soon after preadipocytes are stimulated by differentiation inducers and a necessary step for adipocyte differentiation. However, the effect of C/EBPdelta, another member of the C/EBP family, on MCE remains unclear. In the present study, we investigated the role of C/EBPdelta in the early stages of adipogenesis. A remarkable induction of C/EBPdelta gene expression after the initiation of differentiation was observed not in proliferating preadipocytes, but in growth-arrested, differentiable cells. RNAi-mediated knockdown of C/EBPdelta dramatically suppressed cell growth after differentiation was induced, and inhibited conversion into lipid-laden adipocytes. Furthermore, silencing of C/EBPdelta impaired the expression of factor for adipocyte differentiation (fad) 49, which is up-regulated and plays a crucial role early in adipogenesis. Taken together, these findings show that C/EBPdelta is involved in MCE and gene expression in the early stages of adipocyte differentiation. SN - 1638-6183 UR - https://www.unboundmedicine.com/medline/citation/19233245/The_role_of_C/EBPdelta_in_the_early_stages_of_adipogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(09)00046-7 DB - PRIME DP - Unbound Medicine ER -