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Culture-independent characterization of bacterial communities associated with the cold-water coral Lophelia pertusa in the northeastern Gulf of Mexico.
Appl Environ Microbiol. 2009 Apr; 75(8):2294-303.AE

Abstract

Bacteria are recognized as an important part of the total biology of shallow-water corals. Studies of shallow-water corals suggest that associated bacteria may benefit the corals by cycling carbon, fixing nitrogen, chelating iron, and producing antibiotics that protect the coral from other microbes. Cold-water or deep-sea corals have a fundamentally different ecology due to their adaptation to cold, dark, high-pressure environments and as such have novel microbiota. The goal of this study was to characterize the microbial associates of Lophelia pertusa in the northeastern Gulf of Mexico. This is the first study to collect the coral samples in individual insulated containers and to preserve coral samples at depth in an effort to minimize thermal shock and evaluate the effects of environmental gradients on the microbial diversity of samples. Molecular analysis of bacterial diversity showed a marked difference between the two study sites, Viosca Knoll 906/862 (VK906/862) and Viosca Knoll 826 (VK826). The bacterial communities from VK826 were dominated by a variety of unknown mycoplasmal members of the Tenericutes and Bacteroidetes, whereas the libraries from VK906/862 were dominated by members of the Proteobacteria. In addition to novel sequences, the 16S rRNA gene clone libraries revealed many bacterial sequences in common between Gulf of Mexico Lophelia corals and Norwegian fjord Lophelia corals, as well as shallow-water corals. Two Lophelia-specific bacterial groups were identified: a cluster of gammaproteobacteria related to sulfide-oxidizing gill symbionts of seep clams and a group of Mycoplasma spp. The presence of these groups in both Gulf and Norwegian Lophelia corals indicates that in spite of the geographic heterogeneity observed in Lophelia-associated bacterial communities, there are Lophelia-specific microbes.

Authors+Show Affiliations

U.S. Geological Survey, 600 4th Street South, St. Petersburg, Florida 33701, USA. ckellogg@usgs.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19233949

Citation

Kellogg, Christina A., et al. "Culture-independent Characterization of Bacterial Communities Associated With the Cold-water Coral Lophelia Pertusa in the Northeastern Gulf of Mexico." Applied and Environmental Microbiology, vol. 75, no. 8, 2009, pp. 2294-303.
Kellogg CA, Lisle JT, Galkiewicz JP. Culture-independent characterization of bacterial communities associated with the cold-water coral Lophelia pertusa in the northeastern Gulf of Mexico. Appl Environ Microbiol. 2009;75(8):2294-303.
Kellogg, C. A., Lisle, J. T., & Galkiewicz, J. P. (2009). Culture-independent characterization of bacterial communities associated with the cold-water coral Lophelia pertusa in the northeastern Gulf of Mexico. Applied and Environmental Microbiology, 75(8), 2294-303. https://doi.org/10.1128/AEM.02357-08
Kellogg CA, Lisle JT, Galkiewicz JP. Culture-independent Characterization of Bacterial Communities Associated With the Cold-water Coral Lophelia Pertusa in the Northeastern Gulf of Mexico. Appl Environ Microbiol. 2009;75(8):2294-303. PubMed PMID: 19233949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Culture-independent characterization of bacterial communities associated with the cold-water coral Lophelia pertusa in the northeastern Gulf of Mexico. AU - Kellogg,Christina A, AU - Lisle,John T, AU - Galkiewicz,Julia P, Y1 - 2009/02/20/ PY - 2009/2/24/entrez PY - 2009/2/24/pubmed PY - 2009/5/12/medline SP - 2294 EP - 303 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 75 IS - 8 N2 - Bacteria are recognized as an important part of the total biology of shallow-water corals. Studies of shallow-water corals suggest that associated bacteria may benefit the corals by cycling carbon, fixing nitrogen, chelating iron, and producing antibiotics that protect the coral from other microbes. Cold-water or deep-sea corals have a fundamentally different ecology due to their adaptation to cold, dark, high-pressure environments and as such have novel microbiota. The goal of this study was to characterize the microbial associates of Lophelia pertusa in the northeastern Gulf of Mexico. This is the first study to collect the coral samples in individual insulated containers and to preserve coral samples at depth in an effort to minimize thermal shock and evaluate the effects of environmental gradients on the microbial diversity of samples. Molecular analysis of bacterial diversity showed a marked difference between the two study sites, Viosca Knoll 906/862 (VK906/862) and Viosca Knoll 826 (VK826). The bacterial communities from VK826 were dominated by a variety of unknown mycoplasmal members of the Tenericutes and Bacteroidetes, whereas the libraries from VK906/862 were dominated by members of the Proteobacteria. In addition to novel sequences, the 16S rRNA gene clone libraries revealed many bacterial sequences in common between Gulf of Mexico Lophelia corals and Norwegian fjord Lophelia corals, as well as shallow-water corals. Two Lophelia-specific bacterial groups were identified: a cluster of gammaproteobacteria related to sulfide-oxidizing gill symbionts of seep clams and a group of Mycoplasma spp. The presence of these groups in both Gulf and Norwegian Lophelia corals indicates that in spite of the geographic heterogeneity observed in Lophelia-associated bacterial communities, there are Lophelia-specific microbes. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/19233949/Culture_independent_characterization_of_bacterial_communities_associated_with_the_cold_water_coral_Lophelia_pertusa_in_the_northeastern_Gulf_of_Mexico_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=19233949 DB - PRIME DP - Unbound Medicine ER -