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Modifications of blood platelet proteins of patients with schizophrenia.
Platelets 2009; 20(2):90-6P

Abstract

Oxidative damage to lipids in plasma, blood platelets and neurons in patients with schizophrenia was described. The aim of our present study was to evaluate oxidative/nitrative modifications of blood platelets proteins by measurement the level of biomarkers of oxidative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with schizophrenia and compare with a control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and competition ELISA, respectively. The method with 5,5'-dithio-bis(2-nitro-benzoic acid) has been used to analyse thiol groups in platelet proteins. We demonstrated for the first time in platelet proteins from patients with schizophrenia a statistically significant increase of the level of biomarkers of oxidative/nitrative stress such as carbonyl groups or 3-nitrotyrosine; in schizophrenic patients the amount of thiol groups in platelet proteins was lower than in platelets from healthy subjects. Our results strongly indicate that in patients with schizophrenia reactive oxygen species and reactive nitrogen species induce not only peroxidation of lipids, but also may stimulate oxidative/nitrative modifications of platelet proteins. The consequence of these modifications may be the alteration of platelet protein structure and function.

Authors+Show Affiliations

II Department of Psychiatry, Medical University of Lodz, Lodz, Poland. tzn_lodz@post.plNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19235050

Citation

Dietrich-Muszalska, Anna, and Beata Olas. "Modifications of Blood Platelet Proteins of Patients With Schizophrenia." Platelets, vol. 20, no. 2, 2009, pp. 90-6.
Dietrich-Muszalska A, Olas B. Modifications of blood platelet proteins of patients with schizophrenia. Platelets. 2009;20(2):90-6.
Dietrich-Muszalska, A., & Olas, B. (2009). Modifications of blood platelet proteins of patients with schizophrenia. Platelets, 20(2), pp. 90-6. doi:10.1080/09537100802641499.
Dietrich-Muszalska A, Olas B. Modifications of Blood Platelet Proteins of Patients With Schizophrenia. Platelets. 2009;20(2):90-6. PubMed PMID: 19235050.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modifications of blood platelet proteins of patients with schizophrenia. AU - Dietrich-Muszalska,Anna, AU - Olas,Beata, PY - 2009/2/24/entrez PY - 2009/2/24/pubmed PY - 2009/3/25/medline SP - 90 EP - 6 JF - Platelets JO - Platelets VL - 20 IS - 2 N2 - Oxidative damage to lipids in plasma, blood platelets and neurons in patients with schizophrenia was described. The aim of our present study was to evaluate oxidative/nitrative modifications of blood platelets proteins by measurement the level of biomarkers of oxidative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with schizophrenia and compare with a control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and competition ELISA, respectively. The method with 5,5'-dithio-bis(2-nitro-benzoic acid) has been used to analyse thiol groups in platelet proteins. We demonstrated for the first time in platelet proteins from patients with schizophrenia a statistically significant increase of the level of biomarkers of oxidative/nitrative stress such as carbonyl groups or 3-nitrotyrosine; in schizophrenic patients the amount of thiol groups in platelet proteins was lower than in platelets from healthy subjects. Our results strongly indicate that in patients with schizophrenia reactive oxygen species and reactive nitrogen species induce not only peroxidation of lipids, but also may stimulate oxidative/nitrative modifications of platelet proteins. The consequence of these modifications may be the alteration of platelet protein structure and function. SN - 1369-1635 UR - https://www.unboundmedicine.com/medline/citation/19235050/Modifications_of_blood_platelet_proteins_of_patients_with_schizophrenia_ L2 - http://www.tandfonline.com/doi/full/10.1080/09537100802641499 DB - PRIME DP - Unbound Medicine ER -