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Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae.
Yeast. 2009 Feb; 26(2):95-110.Y

Abstract

Previous studies from this laboratory indicated that the product of the RSF1 gene of S. cerevisiae is present in both nucleus and mitochondria, and they suggested that Rsf1p acts as a transcriptional modulator. To investigate this latter question, we performed transcriptome profiling of an rsf1 mutant strain and its wild-type parent during a shift from glucose-based fermentative to glycerol-based respiratory growth to identify genes whose expression is regulated by Rsf1p. Loss of Rsf1p engendered a decrease in transcript levels from many genes encoding components of the electron transport chain and various other mitochondrially-localized products. The earlier studies further showed that rsf1 cells exhibit a growth defect on medium containing glycerol, but not ethanol, as sole carbon source. Importantly, transcriptome profiling of the rsf1 mutant during shift from glucose- to glycerol-based medium revealed that the product of this gene plays a major role in both orchestration of the transition to, and maintenance of, efficient growth on glycerol as sole carbon source. An increase in transcript levels from genes encoding products that function in the stress response, and an imbalance between expression of genes encoding glycerol anabolic and catabolic enzymes, was observed in the rsf1 mutant during steady-state growth on glycerol- but not ethanol-based medium; this suggests the presence of partially separate transcriptional regulatory systems for transition to respiratory growth on each of these two carbon sources. Genes whose expression is affected by loss of Rsf1p, which lacks a known DNA-binding motif, lack a common DNA sequence motif in their upstream regions. These and other data presented here strongly suggest that the transcriptional effects exerted by Rsf1p are mediated via interaction with other transcription factors.

Authors+Show Affiliations

Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19235764

Citation

Roberts, George G., and Alan P. Hudson. "Rsf1p Is Required for an Efficient Metabolic Shift From Fermentative to Glycerol-based Respiratory Growth in S. Cerevisiae." Yeast (Chichester, England), vol. 26, no. 2, 2009, pp. 95-110.
Roberts GG, Hudson AP. Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae. Yeast. 2009;26(2):95-110.
Roberts, G. G., & Hudson, A. P. (2009). Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae. Yeast (Chichester, England), 26(2), 95-110. https://doi.org/10.1002/yea.1655
Roberts GG, Hudson AP. Rsf1p Is Required for an Efficient Metabolic Shift From Fermentative to Glycerol-based Respiratory Growth in S. Cerevisiae. Yeast. 2009;26(2):95-110. PubMed PMID: 19235764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae. AU - Roberts,George G,3rd AU - Hudson,Alan P, PY - 2009/2/25/entrez PY - 2009/2/25/pubmed PY - 2009/3/26/medline SP - 95 EP - 110 JF - Yeast (Chichester, England) JO - Yeast VL - 26 IS - 2 N2 - Previous studies from this laboratory indicated that the product of the RSF1 gene of S. cerevisiae is present in both nucleus and mitochondria, and they suggested that Rsf1p acts as a transcriptional modulator. To investigate this latter question, we performed transcriptome profiling of an rsf1 mutant strain and its wild-type parent during a shift from glucose-based fermentative to glycerol-based respiratory growth to identify genes whose expression is regulated by Rsf1p. Loss of Rsf1p engendered a decrease in transcript levels from many genes encoding components of the electron transport chain and various other mitochondrially-localized products. The earlier studies further showed that rsf1 cells exhibit a growth defect on medium containing glycerol, but not ethanol, as sole carbon source. Importantly, transcriptome profiling of the rsf1 mutant during shift from glucose- to glycerol-based medium revealed that the product of this gene plays a major role in both orchestration of the transition to, and maintenance of, efficient growth on glycerol as sole carbon source. An increase in transcript levels from genes encoding products that function in the stress response, and an imbalance between expression of genes encoding glycerol anabolic and catabolic enzymes, was observed in the rsf1 mutant during steady-state growth on glycerol- but not ethanol-based medium; this suggests the presence of partially separate transcriptional regulatory systems for transition to respiratory growth on each of these two carbon sources. Genes whose expression is affected by loss of Rsf1p, which lacks a known DNA-binding motif, lack a common DNA sequence motif in their upstream regions. These and other data presented here strongly suggest that the transcriptional effects exerted by Rsf1p are mediated via interaction with other transcription factors. SN - 1097-0061 UR - https://www.unboundmedicine.com/medline/citation/19235764/Rsf1p_is_required_for_an_efficient_metabolic_shift_from_fermentative_to_glycerol_based_respiratory_growth_in_S__cerevisiae_ L2 - https://doi.org/10.1002/yea.1655 DB - PRIME DP - Unbound Medicine ER -