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Full-length sequence of genotype 3 hepatitis E virus derived from a pig in Thailand.
J Med Virol. 2009 Apr; 81(4):657-64.JM

Abstract

Hepatitis E virus (HEV) infection in pigs was investigated in two principal swine farming areas in Thailand. Anti-HEV antibodies and HEV RNA in sera were examined in 258 pigs reared on five commercial farms from age 1 to 6.5 months and sows. Overall, 167 of 258 (64.7%) pigs were positive for anti-HEV IgG, while 20 of 258 (7.75%) had detectable HEV RNA. Sequence analysis of 20 HEV isolates obtained from viremic pigs revealed that they were 92.3-100% identical to each other and had 82.2-88.2% nucleotide similarity to other reported genotype 3 isolates in 415 nucleotide sequences within ORF2 region. Further characterization by sequencing the complete genome of the Thai swine HEV isolate (named Thai-swHEV07) and phylogenetic analysis showed that Thai-swHEV07 segregated into a cluster consisting of swine isolates from Japan, Mongolia, and Kyrgyzstan within the HEV genotype 3. The Thai-swHEV07 had a genomic length of 7,229 nt excluding the polyadenylated region at 3' terminus of the genome. Comparison of Thai-swHEV07 and 27 reported strains of genotype 3 revealed 80.4-85.9% nucleotide identity, with the highest identity of 85.9% to the novel swHEV strain from Mongolia. These findings suggest that genotype 3 HEV isolates are markedly heterogeneous.

Authors+Show Affiliations

Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI).No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19235846

Citation

Siripanyaphinyo, Uamporn, et al. "Full-length Sequence of Genotype 3 Hepatitis E Virus Derived From a Pig in Thailand." Journal of Medical Virology, vol. 81, no. 4, 2009, pp. 657-64.
Siripanyaphinyo U, Laohasinnarong D, Siripanee J, et al. Full-length sequence of genotype 3 hepatitis E virus derived from a pig in Thailand. J Med Virol. 2009;81(4):657-64.
Siripanyaphinyo, U., Laohasinnarong, D., Siripanee, J., Kaeoket, K., Kameoka, M., Ikuta, K., & Sawanpanyalert, P. (2009). Full-length sequence of genotype 3 hepatitis E virus derived from a pig in Thailand. Journal of Medical Virology, 81(4), 657-64. https://doi.org/10.1002/jmv.21428
Siripanyaphinyo U, et al. Full-length Sequence of Genotype 3 Hepatitis E Virus Derived From a Pig in Thailand. J Med Virol. 2009;81(4):657-64. PubMed PMID: 19235846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Full-length sequence of genotype 3 hepatitis E virus derived from a pig in Thailand. AU - Siripanyaphinyo,Uamporn, AU - Laohasinnarong,Dusit, AU - Siripanee,Juthamas, AU - Kaeoket,Kampon, AU - Kameoka,Masanori, AU - Ikuta,Kazuyoshi, AU - Sawanpanyalert,Pathom, PY - 2009/2/25/entrez PY - 2009/2/25/pubmed PY - 2009/3/26/medline SP - 657 EP - 64 JF - Journal of medical virology JO - J Med Virol VL - 81 IS - 4 N2 - Hepatitis E virus (HEV) infection in pigs was investigated in two principal swine farming areas in Thailand. Anti-HEV antibodies and HEV RNA in sera were examined in 258 pigs reared on five commercial farms from age 1 to 6.5 months and sows. Overall, 167 of 258 (64.7%) pigs were positive for anti-HEV IgG, while 20 of 258 (7.75%) had detectable HEV RNA. Sequence analysis of 20 HEV isolates obtained from viremic pigs revealed that they were 92.3-100% identical to each other and had 82.2-88.2% nucleotide similarity to other reported genotype 3 isolates in 415 nucleotide sequences within ORF2 region. Further characterization by sequencing the complete genome of the Thai swine HEV isolate (named Thai-swHEV07) and phylogenetic analysis showed that Thai-swHEV07 segregated into a cluster consisting of swine isolates from Japan, Mongolia, and Kyrgyzstan within the HEV genotype 3. The Thai-swHEV07 had a genomic length of 7,229 nt excluding the polyadenylated region at 3' terminus of the genome. Comparison of Thai-swHEV07 and 27 reported strains of genotype 3 revealed 80.4-85.9% nucleotide identity, with the highest identity of 85.9% to the novel swHEV strain from Mongolia. These findings suggest that genotype 3 HEV isolates are markedly heterogeneous. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/19235846/Full_length_sequence_of_genotype_3_hepatitis_E_virus_derived_from_a_pig_in_Thailand_ L2 - https://doi.org/10.1002/jmv.21428 DB - PRIME DP - Unbound Medicine ER -