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Dopaminergic and serotoninergic deficiencies in young adult rats prenatally exposed to the bacterial lipopolysaccharide.
Brain Res. 2009 Apr 10; 1265:196-204.BR

Abstract

We have reported previously that prenatal bacterial lipopolysaccharide (LPS) exposure at the gestation window of vulnerability could consistently lead to dopamine (DA) neuron loss in the substantia nigra (SN). Thus, we suggested that prenatal LPS exposure might represent as a risk factor for the development of Parkinson's disease (PD). Here, we report that the same exposure could lead to tryptophan hydroxylase (TPH, a serotonin neuron marker) immunoreactive cell loss in the dorsal raphe nucleus (DRN). Twenty two pups born to saline or LPS-injected gravid female rats at E10.5 were used in the current study. Twelve male pups at age of 4 months (6 from each of two prenatal groups) were used for the tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) immunochemistry studies. The other 10 (5 from each of two prenatal groups) males were used in the biochemistry studies. A 29% THir neuron loss in the substantia nigra (F(1,11)=17.573, P=0.002) and a 31% TPHir neuron loss (F(1,11)=44.005, P<0.001) in the DRN were seen. Significant DA and 5-hydroxytryptamine (5-HT) reductions (P<0.05) were found in the frontal cortex, nucleus accumbens, striatum, amygdala, hippocampus, and hypothalamus. The losses of DA and 5-HT were accompanied by the significant increases in homovanillic acid over DA and 5-hydroxyindoleacetic acid over 5-HT ratios in the most areas tested. These data further validate prenatal LPS exposure as a model of PD since DA and 5-HT changes similar to those seen in PD patients. They also suggest that prenatal LPS might be a risk factor for other diseases including mood disorders.

Authors+Show Affiliations

Institute of Population Health Sciences, National Health Research Institutes, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19236855

Citation

Wang, Sabrina, et al. "Dopaminergic and Serotoninergic Deficiencies in Young Adult Rats Prenatally Exposed to the Bacterial Lipopolysaccharide." Brain Research, vol. 1265, 2009, pp. 196-204.
Wang S, Yan JY, Lo YK, et al. Dopaminergic and serotoninergic deficiencies in young adult rats prenatally exposed to the bacterial lipopolysaccharide. Brain Res. 2009;1265:196-204.
Wang, S., Yan, J. Y., Lo, Y. K., Carvey, P. M., & Ling, Z. (2009). Dopaminergic and serotoninergic deficiencies in young adult rats prenatally exposed to the bacterial lipopolysaccharide. Brain Research, 1265, 196-204. https://doi.org/10.1016/j.brainres.2009.02.022
Wang S, et al. Dopaminergic and Serotoninergic Deficiencies in Young Adult Rats Prenatally Exposed to the Bacterial Lipopolysaccharide. Brain Res. 2009 Apr 10;1265:196-204. PubMed PMID: 19236855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dopaminergic and serotoninergic deficiencies in young adult rats prenatally exposed to the bacterial lipopolysaccharide. AU - Wang,Sabrina, AU - Yan,Jia-Ying, AU - Lo,Yu-Kang, AU - Carvey,Paul M, AU - Ling,Zaodung, Y1 - 2009/02/21/ PY - 2008/10/02/received PY - 2009/02/06/revised PY - 2009/02/06/accepted PY - 2009/2/25/entrez PY - 2009/2/25/pubmed PY - 2009/5/14/medline SP - 196 EP - 204 JF - Brain research JO - Brain Res VL - 1265 N2 - We have reported previously that prenatal bacterial lipopolysaccharide (LPS) exposure at the gestation window of vulnerability could consistently lead to dopamine (DA) neuron loss in the substantia nigra (SN). Thus, we suggested that prenatal LPS exposure might represent as a risk factor for the development of Parkinson's disease (PD). Here, we report that the same exposure could lead to tryptophan hydroxylase (TPH, a serotonin neuron marker) immunoreactive cell loss in the dorsal raphe nucleus (DRN). Twenty two pups born to saline or LPS-injected gravid female rats at E10.5 were used in the current study. Twelve male pups at age of 4 months (6 from each of two prenatal groups) were used for the tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) immunochemistry studies. The other 10 (5 from each of two prenatal groups) males were used in the biochemistry studies. A 29% THir neuron loss in the substantia nigra (F(1,11)=17.573, P=0.002) and a 31% TPHir neuron loss (F(1,11)=44.005, P<0.001) in the DRN were seen. Significant DA and 5-hydroxytryptamine (5-HT) reductions (P<0.05) were found in the frontal cortex, nucleus accumbens, striatum, amygdala, hippocampus, and hypothalamus. The losses of DA and 5-HT were accompanied by the significant increases in homovanillic acid over DA and 5-hydroxyindoleacetic acid over 5-HT ratios in the most areas tested. These data further validate prenatal LPS exposure as a model of PD since DA and 5-HT changes similar to those seen in PD patients. They also suggest that prenatal LPS might be a risk factor for other diseases including mood disorders. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19236855/Dopaminergic_and_serotoninergic_deficiencies_in_young_adult_rats_prenatally_exposed_to_the_bacterial_lipopolysaccharide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(09)00301-1 DB - PRIME DP - Unbound Medicine ER -