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Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase.
Acta Biochim Pol. 2009; 56(1):183-7.AB

Abstract

BACKGROUND

Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme.

SUBJECTS AND METHODS

Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 microg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px.

RESULTS

Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein.

CONCLUSIONS

The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.

Authors+Show Affiliations

Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland. bronzach@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19238255

Citation

Zachara, Bronislaw A., et al. "Selenium Supplementation to Chronic Kidney Disease Patients On Hemodialysis Does Not Induce the Synthesis of Plasma Glutathione Peroxidase." Acta Biochimica Polonica, vol. 56, no. 1, 2009, pp. 183-7.
Zachara BA, Gromadzinska J, Zbrog Z, et al. Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase. Acta Biochim Pol. 2009;56(1):183-7.
Zachara, B. A., Gromadzinska, J., Zbrog, Z., Swiech, R., Wasowicz, W., Twardowska, E., Jablonska, E., & Sobala, W. (2009). Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase. Acta Biochimica Polonica, 56(1), 183-7.
Zachara BA, et al. Selenium Supplementation to Chronic Kidney Disease Patients On Hemodialysis Does Not Induce the Synthesis of Plasma Glutathione Peroxidase. Acta Biochim Pol. 2009;56(1):183-7. PubMed PMID: 19238255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase. AU - Zachara,Bronislaw A, AU - Gromadzinska,Jolanta, AU - Zbrog,Zbigniew, AU - Swiech,Rafal, AU - Wasowicz,Wojciech, AU - Twardowska,Ewa, AU - Jablonska,Ewa, AU - Sobala,Wojciech, Y1 - 2009/02/24/ PY - 2008/11/24/received PY - 2009/01/22/revised PY - 2009/02/14/accepted PY - 2009/2/25/entrez PY - 2009/2/25/pubmed PY - 2009/6/20/medline SP - 183 EP - 7 JF - Acta biochimica Polonica JO - Acta Biochim Pol VL - 56 IS - 1 N2 - BACKGROUND: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. SUBJECTS AND METHODS: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 microg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. RESULTS: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. CONCLUSIONS: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium. SN - 0001-527X UR - https://www.unboundmedicine.com/medline/citation/19238255/Selenium_supplementation_to_chronic_kidney_disease_patients_on_hemodialysis_does_not_induce_the_synthesis_of_plasma_glutathione_peroxidase_ L2 - http://www.actabp.pl/pdf/1_2009/183.pdf DB - PRIME DP - Unbound Medicine ER -