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Living fungi cells encapsulated in polyelectrolyte shells doped with metal nanoparticles.
Langmuir. 2009 Apr 21; 25(8):4628-34.L

Abstract

We report the layer-by-layer coating of living fungi cells (Saccharomyces cerevisiae and Trichoderma asperellum) with polyelectrolytes poly(allylamine hydrochloride)/sodium poly(styrene sulfonate) and bovine serum albumin/DNA and citrate-stabilized gold and silver nanoparticles. It was found that the nanoparticles were effectively incorporated between oppositely charged polyelectrolyte layers, modifying the topography and the roughness of cell walls. The formation of large aggregates of nanoparticles on the cell walls of encapsulated cells was shown. It was found that the encapsulated cells preserved their viability and the shells were soft enough to allow the growth of mycelium. The surface-enhanced Raman scattering (SERS) was used to investigate the biochemical environments of the gold and silver nanoparticles immobilized on the surface of T. asperellum conidia. The SERS spectra from encapsulated conidia and polyelectrolytes indicate that both gold and silver nanoparticles interact with cell walls from different locations, and nanoparticle-polyelectrolyte interaction is limited. The approach described in this paper might have potential applications in modification of living cells.

Authors+Show Affiliations

Department of Biochemistry, Kazan State University, Kremlevskaya 18, Kazan 420008, Tatarstan, Russian Federation. biosensor@bk.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19239251

Citation

Fakhrullin, Rawil F., et al. "Living Fungi Cells Encapsulated in Polyelectrolyte Shells Doped With Metal Nanoparticles." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 25, no. 8, 2009, pp. 4628-34.
Fakhrullin RF, Zamaleeva AI, Morozov MV, et al. Living fungi cells encapsulated in polyelectrolyte shells doped with metal nanoparticles. Langmuir. 2009;25(8):4628-34.
Fakhrullin, R. F., Zamaleeva, A. I., Morozov, M. V., Tazetdinova, D. I., Alimova, F. K., Hilmutdinov, A. K., Zhdanov, R. I., Kahraman, M., & Culha, M. (2009). Living fungi cells encapsulated in polyelectrolyte shells doped with metal nanoparticles. Langmuir : the ACS Journal of Surfaces and Colloids, 25(8), 4628-34. https://doi.org/10.1021/la803871z
Fakhrullin RF, et al. Living Fungi Cells Encapsulated in Polyelectrolyte Shells Doped With Metal Nanoparticles. Langmuir. 2009 Apr 21;25(8):4628-34. PubMed PMID: 19239251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Living fungi cells encapsulated in polyelectrolyte shells doped with metal nanoparticles. AU - Fakhrullin,Rawil F, AU - Zamaleeva,Alsu I, AU - Morozov,Mikhail V, AU - Tazetdinova,Diana I, AU - Alimova,Farida K, AU - Hilmutdinov,Albert K, AU - Zhdanov,Renat I, AU - Kahraman,Mehmet, AU - Culha,Mustafa, PY - 2009/2/26/entrez PY - 2009/2/26/pubmed PY - 2009/7/8/medline SP - 4628 EP - 34 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 25 IS - 8 N2 - We report the layer-by-layer coating of living fungi cells (Saccharomyces cerevisiae and Trichoderma asperellum) with polyelectrolytes poly(allylamine hydrochloride)/sodium poly(styrene sulfonate) and bovine serum albumin/DNA and citrate-stabilized gold and silver nanoparticles. It was found that the nanoparticles were effectively incorporated between oppositely charged polyelectrolyte layers, modifying the topography and the roughness of cell walls. The formation of large aggregates of nanoparticles on the cell walls of encapsulated cells was shown. It was found that the encapsulated cells preserved their viability and the shells were soft enough to allow the growth of mycelium. The surface-enhanced Raman scattering (SERS) was used to investigate the biochemical environments of the gold and silver nanoparticles immobilized on the surface of T. asperellum conidia. The SERS spectra from encapsulated conidia and polyelectrolytes indicate that both gold and silver nanoparticles interact with cell walls from different locations, and nanoparticle-polyelectrolyte interaction is limited. The approach described in this paper might have potential applications in modification of living cells. SN - 0743-7463 UR - https://www.unboundmedicine.com/medline/citation/19239251/Living_fungi_cells_encapsulated_in_polyelectrolyte_shells_doped_with_metal_nanoparticles_ L2 - https://doi.org/10.1021/la803871z DB - PRIME DP - Unbound Medicine ER -