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Absence of nasal blockage in a Japanese cedar pollen-induced allergic rhinitis model mouse.
Allergol Int. 2009 Jun; 58(2):171-8.AI

Abstract

BACKGROUND

Japanese cedar pollen-induced allergic rhinitis in a guinea pig model clearly induced not only sneezing but also biphasic nasal blockage. To date, there have only been a few reports on models of murine allergic rhinitis which clearly show nasal blockage. Therefore, in order to try and develop such a model, we administered multiple dosages of intranasal pollen or purified antigen protein Cry j 1.

METHODS

B10.S mice were sensitized by intranasal instillations of either pollen extract or Cry j 1 twice a day for 7 days, which was adsorbed on Al(OH)(3). Subsequently, once a week, the mice were given multiple intranasal instillation challenges of either the pollen suspension or Cry j 1 and the frequency of sneezing was observed after respective challenges were made. Specific airway resistance (sRaw) was measured as an indicator for nasal blockage. Cry j 1-specific IgE levels were measured using an enzyme-linked immunosorbent assay.

RESULTS

The serum Cry j 1-specific IgE level showed clear elevation only in the group sensitized by Cry j 1 + Al(OH)(3) and then challenged by Cry j 1. No elevations were seen in the groups sensitized by pollen extract + Al(OH)(3) followed by a pollen suspension challenge. There was an immediate increase in sneezing after challenges in all of the sensitized-challenged groups. Nevertheless, no increases in sRaw in any of the groups were detected at any of the time points during the 8 hours following the challenges.

CONCLUSIONS

Cry j 1 may be more effective than crude antigens for efficient sensitization/challenge in mice. No increase in sRaw occurred, even in mice that possessed high amounts of Cry j 1-specific IgE and that exhibited sneezing.

Authors+Show Affiliations

Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19240378

Citation

Ogita-Nakanishi, Hiromi, et al. "Absence of Nasal Blockage in a Japanese Cedar Pollen-induced Allergic Rhinitis Model Mouse." Allergology International : Official Journal of the Japanese Society of Allergology, vol. 58, no. 2, 2009, pp. 171-8.
Ogita-Nakanishi H, Nabe T, Mizutani N, et al. Absence of nasal blockage in a Japanese cedar pollen-induced allergic rhinitis model mouse. Allergol Int. 2009;58(2):171-8.
Ogita-Nakanishi, H., Nabe, T., Mizutani, N., Fujii, M., & Kohno, S. (2009). Absence of nasal blockage in a Japanese cedar pollen-induced allergic rhinitis model mouse. Allergology International : Official Journal of the Japanese Society of Allergology, 58(2), 171-8. https://doi.org/10.2332/allergolint.08-OA-0021
Ogita-Nakanishi H, et al. Absence of Nasal Blockage in a Japanese Cedar Pollen-induced Allergic Rhinitis Model Mouse. Allergol Int. 2009;58(2):171-8. PubMed PMID: 19240378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of nasal blockage in a Japanese cedar pollen-induced allergic rhinitis model mouse. AU - Ogita-Nakanishi,Hiromi, AU - Nabe,Takeshi, AU - Mizutani,Nobuaki, AU - Fujii,Masanori, AU - Kohno,Shigekatsu, Y1 - 2009/02/25/ PY - 2008/06/26/received PY - 2008/09/05/accepted PY - 2009/2/26/entrez PY - 2009/2/26/pubmed PY - 2009/12/16/medline SP - 171 EP - 8 JF - Allergology international : official journal of the Japanese Society of Allergology JO - Allergol Int VL - 58 IS - 2 N2 - BACKGROUND: Japanese cedar pollen-induced allergic rhinitis in a guinea pig model clearly induced not only sneezing but also biphasic nasal blockage. To date, there have only been a few reports on models of murine allergic rhinitis which clearly show nasal blockage. Therefore, in order to try and develop such a model, we administered multiple dosages of intranasal pollen or purified antigen protein Cry j 1. METHODS: B10.S mice were sensitized by intranasal instillations of either pollen extract or Cry j 1 twice a day for 7 days, which was adsorbed on Al(OH)(3). Subsequently, once a week, the mice were given multiple intranasal instillation challenges of either the pollen suspension or Cry j 1 and the frequency of sneezing was observed after respective challenges were made. Specific airway resistance (sRaw) was measured as an indicator for nasal blockage. Cry j 1-specific IgE levels were measured using an enzyme-linked immunosorbent assay. RESULTS: The serum Cry j 1-specific IgE level showed clear elevation only in the group sensitized by Cry j 1 + Al(OH)(3) and then challenged by Cry j 1. No elevations were seen in the groups sensitized by pollen extract + Al(OH)(3) followed by a pollen suspension challenge. There was an immediate increase in sneezing after challenges in all of the sensitized-challenged groups. Nevertheless, no increases in sRaw in any of the groups were detected at any of the time points during the 8 hours following the challenges. CONCLUSIONS: Cry j 1 may be more effective than crude antigens for efficient sensitization/challenge in mice. No increase in sRaw occurred, even in mice that possessed high amounts of Cry j 1-specific IgE and that exhibited sneezing. SN - 1440-1592 UR - https://www.unboundmedicine.com/medline/citation/19240378/Absence_of_nasal_blockage_in_a_Japanese_cedar_pollen_induced_allergic_rhinitis_model_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1323-8930(15)30694-8 DB - PRIME DP - Unbound Medicine ER -