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Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins.
Methods Mol Biol. 2009; 492:201-13.MM

Abstract

Electrospray ionization-mass spectrometry (ESI-MS) is a powerful tool for the characterization of intact proteins. However, complex samples require separation in order to obtain clear mass spectra and avoid matrix interaction; capillary electrophoresis (CE) has been shown to be a powerful separation technique for intact proteins. Herein, we present a method based on capillary zone electrophoretic (CZE) separation coupled online with mass spectrometric protein detection. While this approach is suitable for the separation and characterization of various intact proteins, the emphasis is placed on the separation of glycoforms of various and rather complex glycoproteins. The method has been successfully applied to the analysis of glycoproteins, e.g., erythropoietin, fetuin, and alpha-acid glycoprotein.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Neusüss C
Department of Chemistry, University of Aalen, Aalen, Germany.
Pelzing M
No affiliation info available

MeSH

Amino Acid SequenceAnalytic Sample Preparation MethodsElectrophoresis, CapillaryGlycoproteinsHumansMass SpectrometryProtein Isoforms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19241034

Citation

Neusüss, Christian, and Matthias Pelzing. "Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins." Methods in Molecular Biology (Clifton, N.J.), vol. 492, 2009, pp. 201-13.
Neusüss C, Pelzing M. Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins. Methods Mol Biol. 2009;492:201-13.
Neusüss, C., & Pelzing, M. (2009). Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins. Methods in Molecular Biology (Clifton, N.J.), 492, 201-13. https://doi.org/10.1007/978-1-59745-493-3_11
Neusüss C, Pelzing M. Capillary Zone Electrophoresis-mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins. Methods Mol Biol. 2009;492:201-13. PubMed PMID: 19241034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins. AU - Neusüss,Christian, AU - Pelzing,Matthias, PY - 2009/2/26/entrez PY - 2009/2/26/pubmed PY - 2009/3/19/medline SP - 201 EP - 13 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 492 N2 - Electrospray ionization-mass spectrometry (ESI-MS) is a powerful tool for the characterization of intact proteins. However, complex samples require separation in order to obtain clear mass spectra and avoid matrix interaction; capillary electrophoresis (CE) has been shown to be a powerful separation technique for intact proteins. Herein, we present a method based on capillary zone electrophoretic (CZE) separation coupled online with mass spectrometric protein detection. While this approach is suitable for the separation and characterization of various intact proteins, the emphasis is placed on the separation of glycoforms of various and rather complex glycoproteins. The method has been successfully applied to the analysis of glycoproteins, e.g., erythropoietin, fetuin, and alpha-acid glycoprotein. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19241034/Capillary_zone_electrophoresis_mass_spectrometry_for_the_characterization_of_isoforms_of_intact_glycoproteins_ L2 - https://dx.doi.org/10.1007/978-1-59745-493-3_11 DB - PRIME DP - Unbound Medicine ER -