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Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection.
Anal Bioanal Chem. 2009 Apr; 393(8):2059-66.AB

Abstract

Serotonin (5-hydroxytryptamine, 5-HT) plays vital roles in regulating gastrointestinal functions. Thus, the detection of 5-HT in the gastrointestinal tract is of great importance for biomedical research, medical diagnosis, and pharmaceutical therapy. This paper presents a simple, sensitive, and fast method for the quantification of luminally released serotonin in the feces and tissues of the rat proximal colon by means of capillary electrophoresis with laser-induced fluorescence detection. 5-Carboxyfluorescein N-succinimidyl ester was used for precolumn derivatization of serotonin. The optimal separation and detection conditions were obtained with an electrophoretic buffer containing 60 mM borate (pH 8.90) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). The serotonin concentrations in the feces and tissues of proximal colons were analyzed with this method, and the average values of serotonin in the feces samples were 1.951 +/- 0.446 ng/mg (male) and 2.095 +/- 0.533 ng/mg (female) and 1.397 +/- 0.267 ng/mg in rat proximal colon tissues. The results demonstrate that this method can accurately determine luminally released 5-HT in rats.

Authors+Show Affiliations

School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19242684

Citation

Qi, Sheng-da, et al. "Quantification of Luminally Released Serotonin in Rat Proximal Colon By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Analytical and Bioanalytical Chemistry, vol. 393, no. 8, 2009, pp. 2059-66.
Qi SD, Tian SL, Xu HX, et al. Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2009;393(8):2059-66.
Qi, S. D., Tian, S. L., Xu, H. X., Sung, J. J., & Bian, Z. X. (2009). Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Analytical and Bioanalytical Chemistry, 393(8), 2059-66. https://doi.org/10.1007/s00216-009-2655-6
Qi SD, et al. Quantification of Luminally Released Serotonin in Rat Proximal Colon By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Anal Bioanal Chem. 2009;393(8):2059-66. PubMed PMID: 19242684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. AU - Qi,Sheng-da, AU - Tian,Shun-lian, AU - Xu,Hong-xi, AU - Sung,Joseph J Y, AU - Bian,Zhao-xiang, Y1 - 2009/02/26/ PY - 2008/11/16/received PY - 2009/01/26/accepted PY - 2008/12/31/revised PY - 2009/2/27/entrez PY - 2009/2/27/pubmed PY - 2009/6/24/medline SP - 2059 EP - 66 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 393 IS - 8 N2 - Serotonin (5-hydroxytryptamine, 5-HT) plays vital roles in regulating gastrointestinal functions. Thus, the detection of 5-HT in the gastrointestinal tract is of great importance for biomedical research, medical diagnosis, and pharmaceutical therapy. This paper presents a simple, sensitive, and fast method for the quantification of luminally released serotonin in the feces and tissues of the rat proximal colon by means of capillary electrophoresis with laser-induced fluorescence detection. 5-Carboxyfluorescein N-succinimidyl ester was used for precolumn derivatization of serotonin. The optimal separation and detection conditions were obtained with an electrophoretic buffer containing 60 mM borate (pH 8.90) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). The serotonin concentrations in the feces and tissues of proximal colons were analyzed with this method, and the average values of serotonin in the feces samples were 1.951 +/- 0.446 ng/mg (male) and 2.095 +/- 0.533 ng/mg (female) and 1.397 +/- 0.267 ng/mg in rat proximal colon tissues. The results demonstrate that this method can accurately determine luminally released 5-HT in rats. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/19242684/Quantification_of_luminally_released_serotonin_in_rat_proximal_colon_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://dx.doi.org/10.1007/s00216-009-2655-6 DB - PRIME DP - Unbound Medicine ER -