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Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays.
Yeast. 2009 Mar; 26(3):147-65.Y

Abstract

The bottom fermenting yeast Saccharomyces pastorianus is reported to have arisen as a natural hybrid of two yeast strains, S. cerevisiae and S. bayanus. The S. pastorianus genome includes S. cerevisiae-type (Sc-type) genes and orthologous lager-fermenting-yeast specific-type (Lg-type) genes derived from S. cerevisiae and S. bayanus, respectively. To gain insights into the physiological properties of S. pastorianus, we developed an in situ synthesized 60-mer oligonucleotide microarray for gene expression monitoring of these orthologous genes, consisting of approximately 6600 Sc-type genes and 3200 Lg-type genes. A comparison of the transcriptional profile of orthologous genes (e.g. Sc-type and Lg-type genes) in S. cerevisiae or S. bayanus demonstrated the feasibility of performing gene expression studies with this microarray. Genome-wide analysis of S. pastorianus with this microarray could clearly distinguish more than 67% of the expressed orthologous genes. Furthermore, it was shown that the gene expression of particular Lg-type genes differed from that of the orthologous Sc-type genes, suggesting that some Lg-type and Sc-type genes may have different functional roles. We conclude that the oligonucleotide microarray that we constructed is a powerful tool for the monitoring of gene expression of the orthologous genes of S. pastorianus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Minato T
Central Laboratories for Frontier Technology, Kirin Holdings Co Ltd, Yokohama-shi, Kanagawa, Japan.
Yoshida S
No affiliation info available
Ishiguro T
No affiliation info available
Shimada E
No affiliation info available
Mizutani S
No affiliation info available
Kobayashi O
No affiliation info available
Yoshimoto H
No affiliation info available

MeSH

BeerDNA, FungalFermentationFungal ProteinsGene Expression ProfilingGene Expression Regulation, FungalOligonucleotide Array Sequence AnalysisOligonucleotide ProbesReverse Transcriptase Polymerase Chain ReactionSaccharomycesSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19243081

Citation

Minato, Toshiko, et al. "Expression Profiling of the Bottom Fermenting Yeast Saccharomyces Pastorianus Orthologous Genes Using Oligonucleotide Microarrays." Yeast (Chichester, England), vol. 26, no. 3, 2009, pp. 147-65.
Minato T, Yoshida S, Ishiguro T, et al. Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays. Yeast. 2009;26(3):147-65.
Minato, T., Yoshida, S., Ishiguro, T., Shimada, E., Mizutani, S., Kobayashi, O., & Yoshimoto, H. (2009). Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays. Yeast (Chichester, England), 26(3), 147-65. https://doi.org/10.1002/yea.1654
Minato T, et al. Expression Profiling of the Bottom Fermenting Yeast Saccharomyces Pastorianus Orthologous Genes Using Oligonucleotide Microarrays. Yeast. 2009;26(3):147-65. PubMed PMID: 19243081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiling of the bottom fermenting yeast Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays. AU - Minato,Toshiko, AU - Yoshida,Satoshi, AU - Ishiguro,Tatsuji, AU - Shimada,Emiko, AU - Mizutani,Satoru, AU - Kobayashi,Osamu, AU - Yoshimoto,Hiroyuki, PY - 2009/2/27/entrez PY - 2009/2/27/pubmed PY - 2009/3/26/medline SP - 147 EP - 65 JF - Yeast (Chichester, England) JO - Yeast VL - 26 IS - 3 N2 - The bottom fermenting yeast Saccharomyces pastorianus is reported to have arisen as a natural hybrid of two yeast strains, S. cerevisiae and S. bayanus. The S. pastorianus genome includes S. cerevisiae-type (Sc-type) genes and orthologous lager-fermenting-yeast specific-type (Lg-type) genes derived from S. cerevisiae and S. bayanus, respectively. To gain insights into the physiological properties of S. pastorianus, we developed an in situ synthesized 60-mer oligonucleotide microarray for gene expression monitoring of these orthologous genes, consisting of approximately 6600 Sc-type genes and 3200 Lg-type genes. A comparison of the transcriptional profile of orthologous genes (e.g. Sc-type and Lg-type genes) in S. cerevisiae or S. bayanus demonstrated the feasibility of performing gene expression studies with this microarray. Genome-wide analysis of S. pastorianus with this microarray could clearly distinguish more than 67% of the expressed orthologous genes. Furthermore, it was shown that the gene expression of particular Lg-type genes differed from that of the orthologous Sc-type genes, suggesting that some Lg-type and Sc-type genes may have different functional roles. We conclude that the oligonucleotide microarray that we constructed is a powerful tool for the monitoring of gene expression of the orthologous genes of S. pastorianus. SN - 1097-0061 UR - https://www.unboundmedicine.com/medline/citation/19243081/Expression_profiling_of_the_bottom_fermenting_yeast_Saccharomyces_pastorianus_orthologous_genes_using_oligonucleotide_microarrays_ L2 - https://doi.org/10.1002/yea.1654 DB - PRIME DP - Unbound Medicine ER -