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Phenotypic and genotypic traits of Shiga toxin-negative E. coli O157:H7/H(-) bovine and porcine strains.
Foodborne Pathog Dis. 2009 Mar; 6(2):235-43.FP

Abstract

Enterohemorrhagic E. coli (EHEC) O157:H7/H(-) (nonmotile) exist as Shiga toxin gene (stx)-positive and stx-negative variants in patients and the environment. We analyzed molecular characteristics, phenotypes, and the phylogenetic background of three stx-negative E. coli O157:H7/H(-) strains isolated from cattle and a pig and compared them with those of human EHEC and stx-negative E. coli O157:H7/H(-). All three animal strains contained fliCH7 and two contained eae. One eae-positive strain (O157:H(-)) was sorbitol-fermenting (SF) and the other (O157:H7) was non-sorbitol-fermenting (nSF). These two strains shared a spectrum of non-stx putative virulence and fitness genes with human nSF and SF EHEC and stx-negative E. coli O157:H7/H(-) and belonged, similar to the vast majority of human isolates, to sequence type (ST) 11 in multilocus sequence typing. In contrast, the eae-negative O157:H7 animal isolate, which was SF, differed in spectrum of virulence genes and also differed phylogenetically (ST717) from the two eae-positive strains and the human EHEC and stx-negative E. coli O157:H7/H(-). In contrast to efforts with human stx-negative E. coli O157:H(-), attempts to transduce the two stx-negative/eae-positive animal O157:H7/H(-) strains with stx(2)-encoding phages from human SF and nSF EHEC O157:H7/H(-) failed, despite the animal strains having intact loci where such phages integrate in human EHEC O157 (wrbA and yecE). The role of animal stx-negative/eae-positive and stx-negative/eae-negative E. coli O157:H7/H(-) in their natural source and in human infections needs further investigation.

Authors+Show Affiliations

Institute for Food Safety and Hygiene, University of Zurich, Switzerland. stephanr@fsafety.uzh.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19245340

Citation

Stephan, Roger, et al. "Phenotypic and Genotypic Traits of Shiga Toxin-negative E. Coli O157:H7/H(-) Bovine and Porcine Strains." Foodborne Pathogens and Disease, vol. 6, no. 2, 2009, pp. 235-43.
Stephan R, Zhang W, Bielaszewska M, et al. Phenotypic and genotypic traits of Shiga toxin-negative E. coli O157:H7/H(-) bovine and porcine strains. Foodborne Pathog Dis. 2009;6(2):235-43.
Stephan, R., Zhang, W., Bielaszewska, M., Mellmann, A., & Karch, H. (2009). Phenotypic and genotypic traits of Shiga toxin-negative E. coli O157:H7/H(-) bovine and porcine strains. Foodborne Pathogens and Disease, 6(2), 235-43. https://doi.org/10.1089/fpd.2008.0205
Stephan R, et al. Phenotypic and Genotypic Traits of Shiga Toxin-negative E. Coli O157:H7/H(-) Bovine and Porcine Strains. Foodborne Pathog Dis. 2009;6(2):235-43. PubMed PMID: 19245340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genotypic traits of Shiga toxin-negative E. coli O157:H7/H(-) bovine and porcine strains. AU - Stephan,Roger, AU - Zhang,Wenlan, AU - Bielaszewska,Martina, AU - Mellmann,Alexander, AU - Karch,Helge, PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/6/3/medline SP - 235 EP - 43 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 6 IS - 2 N2 - Enterohemorrhagic E. coli (EHEC) O157:H7/H(-) (nonmotile) exist as Shiga toxin gene (stx)-positive and stx-negative variants in patients and the environment. We analyzed molecular characteristics, phenotypes, and the phylogenetic background of three stx-negative E. coli O157:H7/H(-) strains isolated from cattle and a pig and compared them with those of human EHEC and stx-negative E. coli O157:H7/H(-). All three animal strains contained fliCH7 and two contained eae. One eae-positive strain (O157:H(-)) was sorbitol-fermenting (SF) and the other (O157:H7) was non-sorbitol-fermenting (nSF). These two strains shared a spectrum of non-stx putative virulence and fitness genes with human nSF and SF EHEC and stx-negative E. coli O157:H7/H(-) and belonged, similar to the vast majority of human isolates, to sequence type (ST) 11 in multilocus sequence typing. In contrast, the eae-negative O157:H7 animal isolate, which was SF, differed in spectrum of virulence genes and also differed phylogenetically (ST717) from the two eae-positive strains and the human EHEC and stx-negative E. coli O157:H7/H(-). In contrast to efforts with human stx-negative E. coli O157:H(-), attempts to transduce the two stx-negative/eae-positive animal O157:H7/H(-) strains with stx(2)-encoding phages from human SF and nSF EHEC O157:H7/H(-) failed, despite the animal strains having intact loci where such phages integrate in human EHEC O157 (wrbA and yecE). The role of animal stx-negative/eae-positive and stx-negative/eae-negative E. coli O157:H7/H(-) in their natural source and in human infections needs further investigation. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/19245340/Phenotypic_and_genotypic_traits_of_Shiga_toxin_negative_E__coli_O157:H7/H____bovine_and_porcine_strains_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2008.0205?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -