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Multiple defects in the respiratory chain lead to the repression of genes encoding components of the respiratory chain and TCA cycle enzymes.
J Mol Biol. 2009 Apr 17; 387(5):1081-91.JM

Abstract

Respiratory complexes III, IV and V are formed by components of both nuclear and mitochondrial origin and are embedded in the inner mitochondrial membrane. Their assembly requires the auxiliary factor Oxa1, and the absence of this protein has severe consequences on these three major respiratory chain enzymes. We have studied, in the yeast Saccharomyces cerevisiae, the effect of the loss of Oxa1 function and of other respiratory defects on the expression of nuclear genes encoding components of the respiratory complexes and tricarboxylic acid cycle enzymes. We observed that the concomitant decrease in the level of two respiratory enzymes, complexes III and IV, led to their repression. These genes are known targets of the transcriptional activator complex Hap2/3/4/5 that plays a central role in the reprogramming of yeast metabolism when cells switch from a fermenting, glucose-repressed state to a respiring, derepressed state. We found that the Hap4 protein, the regulatory subunit of the transcriptional complex, was present at a lower level in the oxa1 mutants whereas no change in HAP4 transcript level was observed, suggesting a posttranscriptional modulation. In addition, an altered mitochondrial morphology was observed in mutants with decreased expression of Hap2/3/4/5 target genes. We suggest that the aberrant mitochondrial morphology, presumably caused by the severely decreased level of at least two respiratory enzymes, might be part of the signalling pathway linking the mitochondrial defect and Hap2/3/4/5.

Authors+Show Affiliations

University of Massachusetts Medical School, BMP, LRB, 364 Plantation Street, Worcester, MA 01605, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19245817

Citation

Bourges, Ingrid, et al. "Multiple Defects in the Respiratory Chain Lead to the Repression of Genes Encoding Components of the Respiratory Chain and TCA Cycle Enzymes." Journal of Molecular Biology, vol. 387, no. 5, 2009, pp. 1081-91.
Bourges I, Mucchielli MH, Herbert CJ, et al. Multiple defects in the respiratory chain lead to the repression of genes encoding components of the respiratory chain and TCA cycle enzymes. J Mol Biol. 2009;387(5):1081-91.
Bourges, I., Mucchielli, M. H., Herbert, C. J., Guiard, B., Dujardin, G., & Meunier, B. (2009). Multiple defects in the respiratory chain lead to the repression of genes encoding components of the respiratory chain and TCA cycle enzymes. Journal of Molecular Biology, 387(5), 1081-91. https://doi.org/10.1016/j.jmb.2009.02.039
Bourges I, et al. Multiple Defects in the Respiratory Chain Lead to the Repression of Genes Encoding Components of the Respiratory Chain and TCA Cycle Enzymes. J Mol Biol. 2009 Apr 17;387(5):1081-91. PubMed PMID: 19245817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple defects in the respiratory chain lead to the repression of genes encoding components of the respiratory chain and TCA cycle enzymes. AU - Bourges,Ingrid, AU - Mucchielli,Marie-Helene, AU - Herbert,Christopher J, AU - Guiard,Bernard, AU - Dujardin,Geneviève, AU - Meunier,Brigitte, Y1 - 2009/02/23/ PY - 2008/10/14/received PY - 2009/02/11/revised PY - 2009/02/16/accepted PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/4/25/medline SP - 1081 EP - 91 JF - Journal of molecular biology JO - J Mol Biol VL - 387 IS - 5 N2 - Respiratory complexes III, IV and V are formed by components of both nuclear and mitochondrial origin and are embedded in the inner mitochondrial membrane. Their assembly requires the auxiliary factor Oxa1, and the absence of this protein has severe consequences on these three major respiratory chain enzymes. We have studied, in the yeast Saccharomyces cerevisiae, the effect of the loss of Oxa1 function and of other respiratory defects on the expression of nuclear genes encoding components of the respiratory complexes and tricarboxylic acid cycle enzymes. We observed that the concomitant decrease in the level of two respiratory enzymes, complexes III and IV, led to their repression. These genes are known targets of the transcriptional activator complex Hap2/3/4/5 that plays a central role in the reprogramming of yeast metabolism when cells switch from a fermenting, glucose-repressed state to a respiring, derepressed state. We found that the Hap4 protein, the regulatory subunit of the transcriptional complex, was present at a lower level in the oxa1 mutants whereas no change in HAP4 transcript level was observed, suggesting a posttranscriptional modulation. In addition, an altered mitochondrial morphology was observed in mutants with decreased expression of Hap2/3/4/5 target genes. We suggest that the aberrant mitochondrial morphology, presumably caused by the severely decreased level of at least two respiratory enzymes, might be part of the signalling pathway linking the mitochondrial defect and Hap2/3/4/5. SN - 1089-8638 UR - https://www.unboundmedicine.com/medline/citation/19245817/Multiple_defects_in_the_respiratory_chain_lead_to_the_repression_of_genes_encoding_components_of_the_respiratory_chain_and_TCA_cycle_enzymes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(09)00209-5 DB - PRIME DP - Unbound Medicine ER -