TY - JOUR T1 - Micellar electrokinetic biofluid analysis of biogenic amines using on-line sample concentration and UV laser-induced native fluorescence detection. AU - Tseng,Hua-Ming, AU - Barrett,David A, Y1 - 2009/02/11/ PY - 2008/09/12/received PY - 2009/02/02/revised PY - 2009/02/05/accepted PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/9/11/medline SP - 3387 EP - 91 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 15 N2 - A simple and sensitive sweeping micellar electrokinetic chromatography method coupled with UV laser-induced native fluorescence detection has been developed for quantitative analysis of biogenic amines in biofluids. The background electrolyte comprised 30 mmol L(-1) phosphoric acid and 20 mmol L(-1) sodium dodecyl sulfate. The concentration limits of detection of analytes using sweeping-micellar electrokinetic chromatography (sweeping-MEKC) were in the range 7-100 nmol L(-1), which were 250-3600-fold improvement for dopamine, DOPA and epinephrine compared with conventional capillary zone electrophoresis. An improvement of approximately 20-fold was observed for all analytes compared with typical micellar electrokinetic chromatography conditions. Baseline separation was achieved for the all analytes within 12 min and migration-time and peak-area repeatability were better than RSD 0.35% and 5.68%, respectively. The developed method was applied to measure the biogenic amines in biofluids extracted from wheat phloem sap, human plasma and human urine. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19246044/Micellar_electrokinetic_biofluid_analysis_of_biogenic_amines_using_on_line_sample_concentration_and_UV_laser_induced_native_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00218-0 DB - PRIME DP - Unbound Medicine ER -