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Micellar electrokinetic biofluid analysis of biogenic amines using on-line sample concentration and UV laser-induced native fluorescence detection.
J Chromatogr A. 2009 Apr 10; 1216(15):3387-91.JC

Abstract

A simple and sensitive sweeping micellar electrokinetic chromatography method coupled with UV laser-induced native fluorescence detection has been developed for quantitative analysis of biogenic amines in biofluids. The background electrolyte comprised 30 mmol L(-1) phosphoric acid and 20 mmol L(-1) sodium dodecyl sulfate. The concentration limits of detection of analytes using sweeping-micellar electrokinetic chromatography (sweeping-MEKC) were in the range 7-100 nmol L(-1), which were 250-3600-fold improvement for dopamine, DOPA and epinephrine compared with conventional capillary zone electrophoresis. An improvement of approximately 20-fold was observed for all analytes compared with typical micellar electrokinetic chromatography conditions. Baseline separation was achieved for the all analytes within 12 min and migration-time and peak-area repeatability were better than RSD 0.35% and 5.68%, respectively. The developed method was applied to measure the biogenic amines in biofluids extracted from wheat phloem sap, human plasma and human urine.

Authors+Show Affiliations

Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham, UK.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19246044

Citation

Tseng, Hua-Ming, and David A. Barrett. "Micellar Electrokinetic Biofluid Analysis of Biogenic Amines Using On-line Sample Concentration and UV Laser-induced Native Fluorescence Detection." Journal of Chromatography. A, vol. 1216, no. 15, 2009, pp. 3387-91.
Tseng HM, Barrett DA. Micellar electrokinetic biofluid analysis of biogenic amines using on-line sample concentration and UV laser-induced native fluorescence detection. J Chromatogr A. 2009;1216(15):3387-91.
Tseng, H. M., & Barrett, D. A. (2009). Micellar electrokinetic biofluid analysis of biogenic amines using on-line sample concentration and UV laser-induced native fluorescence detection. Journal of Chromatography. A, 1216(15), 3387-91. https://doi.org/10.1016/j.chroma.2009.02.018
Tseng HM, Barrett DA. Micellar Electrokinetic Biofluid Analysis of Biogenic Amines Using On-line Sample Concentration and UV Laser-induced Native Fluorescence Detection. J Chromatogr A. 2009 Apr 10;1216(15):3387-91. PubMed PMID: 19246044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic biofluid analysis of biogenic amines using on-line sample concentration and UV laser-induced native fluorescence detection. AU - Tseng,Hua-Ming, AU - Barrett,David A, Y1 - 2009/02/11/ PY - 2008/09/12/received PY - 2009/02/02/revised PY - 2009/02/05/accepted PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/9/11/medline SP - 3387 EP - 91 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 15 N2 - A simple and sensitive sweeping micellar electrokinetic chromatography method coupled with UV laser-induced native fluorescence detection has been developed for quantitative analysis of biogenic amines in biofluids. The background electrolyte comprised 30 mmol L(-1) phosphoric acid and 20 mmol L(-1) sodium dodecyl sulfate. The concentration limits of detection of analytes using sweeping-micellar electrokinetic chromatography (sweeping-MEKC) were in the range 7-100 nmol L(-1), which were 250-3600-fold improvement for dopamine, DOPA and epinephrine compared with conventional capillary zone electrophoresis. An improvement of approximately 20-fold was observed for all analytes compared with typical micellar electrokinetic chromatography conditions. Baseline separation was achieved for the all analytes within 12 min and migration-time and peak-area repeatability were better than RSD 0.35% and 5.68%, respectively. The developed method was applied to measure the biogenic amines in biofluids extracted from wheat phloem sap, human plasma and human urine. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19246044/Micellar_electrokinetic_biofluid_analysis_of_biogenic_amines_using_on_line_sample_concentration_and_UV_laser_induced_native_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00218-0 DB - PRIME DP - Unbound Medicine ER -