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Excessive energy intake does not modify fed-state tissue protein synthesis rates in adult rats.
Obesity (Silver Spring). 2009 Jul; 17(7):1348-55.O

Abstract

The impact of chronic excessive energy intake on protein metabolism is still controversial. Male Wistar rats were fed ad libitum during 5 weeks with either a high-fat high-sucrose diet (HF: n = 9) containing 45% of total energy as lipids (protein 14%; carbohydrate 40% with 83.5% sucrose) or a standard diet (controls: n = 10). Energy intake and body weight were recorded. At the end of the experiment, we measured body composition, metabolic parameters (plasma amino acid, lipid, insulin, and glucose levels), inflammatory parameter (plasma alpha2-macroglobulin), oxidative stress parameters (antioxidant enzyme activities, lipoperoxidation (LPO), protein carbonyl content in liver and muscle), and in vivo fed-state fractional protein synthesis rates (FSRs) in muscle and liver. Energy intake was significantly higher in HF compared with control rats (+28%). There were significant increases in body weight (+8%), body fat (+21%), renal (+41%), and epidydimal (+28%) fat pads in HF compared with control rats. No effect was observed in other tissue weights (liver, muscle, spleen, kidneys, intestine). Liver and muscle FSRs, plasma levels of lipids, glucose, insulin and alpha2-macroglobulin, soleus and liver glutathione reductase and peroxidase activities, MnSOD activity, LPO, and protein carbonyl content were not altered by the HF diet. Only soleus muscle and liver Cu/ZnSOD activity and soleus muscle catalase activities were reduced in HF rats compared with control rats. Thus, chronic excessive energy intake and increased adiposity, in the absence of other metabolic alterations, do not stimulate fed-state tissue protein synthesis rates.

Authors+Show Affiliations

INRA, UMR 1019 Nutrition Humaine, Saint Genès Champanelle, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19247270

Citation

Adéchian, Solange, et al. "Excessive Energy Intake Does Not Modify Fed-state Tissue Protein Synthesis Rates in Adult Rats." Obesity (Silver Spring, Md.), vol. 17, no. 7, 2009, pp. 1348-55.
Adéchian S, Giardina S, Rémond D, et al. Excessive energy intake does not modify fed-state tissue protein synthesis rates in adult rats. Obesity (Silver Spring). 2009;17(7):1348-55.
Adéchian, S., Giardina, S., Rémond, D., Papet, I., Buonocore, D., Gaudichon, C., Dardevet, D., Marzatico, F., & Mosoni, L. (2009). Excessive energy intake does not modify fed-state tissue protein synthesis rates in adult rats. Obesity (Silver Spring, Md.), 17(7), 1348-55. https://doi.org/10.1038/oby.2009.35
Adéchian S, et al. Excessive Energy Intake Does Not Modify Fed-state Tissue Protein Synthesis Rates in Adult Rats. Obesity (Silver Spring). 2009;17(7):1348-55. PubMed PMID: 19247270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excessive energy intake does not modify fed-state tissue protein synthesis rates in adult rats. AU - Adéchian,Solange, AU - Giardina,Silvana, AU - Rémond,Didier, AU - Papet,Isabelle, AU - Buonocore,Daniela, AU - Gaudichon,Claire, AU - Dardevet,Dominique, AU - Marzatico,Fulvio, AU - Mosoni,Laurent, Y1 - 2009/02/26/ PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/10/9/medline SP - 1348 EP - 55 JF - Obesity (Silver Spring, Md.) JO - Obesity (Silver Spring) VL - 17 IS - 7 N2 - The impact of chronic excessive energy intake on protein metabolism is still controversial. Male Wistar rats were fed ad libitum during 5 weeks with either a high-fat high-sucrose diet (HF: n = 9) containing 45% of total energy as lipids (protein 14%; carbohydrate 40% with 83.5% sucrose) or a standard diet (controls: n = 10). Energy intake and body weight were recorded. At the end of the experiment, we measured body composition, metabolic parameters (plasma amino acid, lipid, insulin, and glucose levels), inflammatory parameter (plasma alpha2-macroglobulin), oxidative stress parameters (antioxidant enzyme activities, lipoperoxidation (LPO), protein carbonyl content in liver and muscle), and in vivo fed-state fractional protein synthesis rates (FSRs) in muscle and liver. Energy intake was significantly higher in HF compared with control rats (+28%). There were significant increases in body weight (+8%), body fat (+21%), renal (+41%), and epidydimal (+28%) fat pads in HF compared with control rats. No effect was observed in other tissue weights (liver, muscle, spleen, kidneys, intestine). Liver and muscle FSRs, plasma levels of lipids, glucose, insulin and alpha2-macroglobulin, soleus and liver glutathione reductase and peroxidase activities, MnSOD activity, LPO, and protein carbonyl content were not altered by the HF diet. Only soleus muscle and liver Cu/ZnSOD activity and soleus muscle catalase activities were reduced in HF rats compared with control rats. Thus, chronic excessive energy intake and increased adiposity, in the absence of other metabolic alterations, do not stimulate fed-state tissue protein synthesis rates. SN - 1930-7381 UR - https://www.unboundmedicine.com/medline/citation/19247270/Excessive_energy_intake_does_not_modify_fed_state_tissue_protein_synthesis_rates_in_adult_rats_ L2 - https://doi.org/10.1038/oby.2009.35 DB - PRIME DP - Unbound Medicine ER -