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Generation and functional characterization of mice with a conditional BMP7 allele.
Int J Dev Biol. 2009; 53(4):597-603.IJ

Abstract

Bone Morphogenetic Proteins (BMPs) play multiple and important roles in embryonic development as well as in homeostasis and tissue repair in the adult. Bmp7 has been implicated in developmental disorders and in a variety of diseases, but functional studies to elucidate its role so far have been hampered, since mice deficient in BMP7 die around or just after birth. To facilitate such studies, we generated mice in which the Bmp7 gene has been rendered conditional-null by flanking its first coding exon with loxP sites. To this end, we adapted the two-loxP site strategy to Bacterial Homologous Recombination to create a Bacterial Artificial Chromosome-based vector for direct targeting in mouse embryonic stem cells. Functional analysis showed that in vivo, the conditional-null Bmp7(flx/flx) mice are phenotypically wild type, whereas post Cre-mediated recombination, the resulting Bmp7(delta/delta) mice are phenotypically null. Thus, this study validates the usefulness of the Bmp7(flx/flx) mouse which in turn should empower in vivo studies aimed at elucidating the roles of Bmp7 in postnatal development, homeostasis and disease.

Authors+Show Affiliations

Institute of Immunology, Biomedical Sciences Research Center Al. Fleming, Vari 166 72, Hellas/Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19247966

Citation

Zouvelou, Vasiliki, et al. "Generation and Functional Characterization of Mice With a Conditional BMP7 Allele." The International Journal of Developmental Biology, vol. 53, no. 4, 2009, pp. 597-603.
Zouvelou V, Passa O, Segklia K, et al. Generation and functional characterization of mice with a conditional BMP7 allele. Int J Dev Biol. 2009;53(4):597-603.
Zouvelou, V., Passa, O., Segklia, K., Tsalavos, S., Valenzuela, D. M., Economides, A. N., & Graf, D. (2009). Generation and functional characterization of mice with a conditional BMP7 allele. The International Journal of Developmental Biology, 53(4), 597-603. https://doi.org/10.1387/ijdb.082648vz
Zouvelou V, et al. Generation and Functional Characterization of Mice With a Conditional BMP7 Allele. Int J Dev Biol. 2009;53(4):597-603. PubMed PMID: 19247966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation and functional characterization of mice with a conditional BMP7 allele. AU - Zouvelou,Vasiliki, AU - Passa,Ourania, AU - Segklia,Katerina, AU - Tsalavos,Sotiris, AU - Valenzuela,David M, AU - Economides,Aris N, AU - Graf,Daniel, PY - 2009/2/28/entrez PY - 2009/2/28/pubmed PY - 2009/8/18/medline SP - 597 EP - 603 JF - The International journal of developmental biology JO - Int. J. Dev. Biol. VL - 53 IS - 4 N2 - Bone Morphogenetic Proteins (BMPs) play multiple and important roles in embryonic development as well as in homeostasis and tissue repair in the adult. Bmp7 has been implicated in developmental disorders and in a variety of diseases, but functional studies to elucidate its role so far have been hampered, since mice deficient in BMP7 die around or just after birth. To facilitate such studies, we generated mice in which the Bmp7 gene has been rendered conditional-null by flanking its first coding exon with loxP sites. To this end, we adapted the two-loxP site strategy to Bacterial Homologous Recombination to create a Bacterial Artificial Chromosome-based vector for direct targeting in mouse embryonic stem cells. Functional analysis showed that in vivo, the conditional-null Bmp7(flx/flx) mice are phenotypically wild type, whereas post Cre-mediated recombination, the resulting Bmp7(delta/delta) mice are phenotypically null. Thus, this study validates the usefulness of the Bmp7(flx/flx) mouse which in turn should empower in vivo studies aimed at elucidating the roles of Bmp7 in postnatal development, homeostasis and disease. SN - 1696-3547 UR - https://www.unboundmedicine.com/medline/citation/19247966/Generation_and_functional_characterization_of_mice_with_a_conditional_BMP7_allele_ L2 - http://www.intjdevbiol.com/paper.php?doi=10.1387/ijdb.082648vz DB - PRIME DP - Unbound Medicine ER -