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Evaluation of reverse-transcriptase PCR as a diagnostic tool to confirm Japanese encephalitis virus infection.
Trans R Soc Trop Med Hyg. 2009 Apr; 103(4):403-6.TR

Abstract

Japanese encephalitis (JE) is a serious central nervous system infection and major public health problem in several countries of Southeast Asia including India. This study evaluated the use of IgM ELISA and reverse-transcriptase (RT)-PCR in blood and cerebrospinal fluid (CSF) samples from acute encephalitis patients for the detection of Japanese encephalitis virus (JEV). Forty-four children suffering from acute encephalitis were enrolled, and 36 were selected from whom both CSF and serum samples were available. Twenty-two of the 36 CSF samples were positive for JEV by IgM ELISA and all were negative by RT-PCR. Twenty-three of the 36 serum samples were positive by IgM ELISA while 28 were positive by RT-PCR. Total positivity for JEV infection in CSF and serum samples was 66.7% (24/36) and 83.3% (30/36) respectively by one or both tests. The overall positivity for JEV infection was 86.1% (31/36). We suggest that the use of RT-PCR in serum samples during the early days of JEV infection may be helpful in confirming diagnosis in those cases which are negative for JEV-specific IgM antibodies in both serum and CSF samples.

Authors+Show Affiliations

Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226 014, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19249068

Citation

Saxena, Vandana, et al. "Evaluation of Reverse-transcriptase PCR as a Diagnostic Tool to Confirm Japanese Encephalitis Virus Infection." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 103, no. 4, 2009, pp. 403-6.
Saxena V, Mishra VK, Dhole TN. Evaluation of reverse-transcriptase PCR as a diagnostic tool to confirm Japanese encephalitis virus infection. Trans R Soc Trop Med Hyg. 2009;103(4):403-6.
Saxena, V., Mishra, V. K., & Dhole, T. N. (2009). Evaluation of reverse-transcriptase PCR as a diagnostic tool to confirm Japanese encephalitis virus infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103(4), 403-6. https://doi.org/10.1016/j.trstmh.2009.01.021
Saxena V, Mishra VK, Dhole TN. Evaluation of Reverse-transcriptase PCR as a Diagnostic Tool to Confirm Japanese Encephalitis Virus Infection. Trans R Soc Trop Med Hyg. 2009;103(4):403-6. PubMed PMID: 19249068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of reverse-transcriptase PCR as a diagnostic tool to confirm Japanese encephalitis virus infection. AU - Saxena,Vandana, AU - Mishra,Virendra K, AU - Dhole,Tapan N, Y1 - 2009/02/26/ PY - 2006/10/08/received PY - 2009/01/22/revised PY - 2009/01/22/accepted PY - 2009/3/3/entrez PY - 2009/3/3/pubmed PY - 2009/7/28/medline SP - 403 EP - 6 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 103 IS - 4 N2 - Japanese encephalitis (JE) is a serious central nervous system infection and major public health problem in several countries of Southeast Asia including India. This study evaluated the use of IgM ELISA and reverse-transcriptase (RT)-PCR in blood and cerebrospinal fluid (CSF) samples from acute encephalitis patients for the detection of Japanese encephalitis virus (JEV). Forty-four children suffering from acute encephalitis were enrolled, and 36 were selected from whom both CSF and serum samples were available. Twenty-two of the 36 CSF samples were positive for JEV by IgM ELISA and all were negative by RT-PCR. Twenty-three of the 36 serum samples were positive by IgM ELISA while 28 were positive by RT-PCR. Total positivity for JEV infection in CSF and serum samples was 66.7% (24/36) and 83.3% (30/36) respectively by one or both tests. The overall positivity for JEV infection was 86.1% (31/36). We suggest that the use of RT-PCR in serum samples during the early days of JEV infection may be helpful in confirming diagnosis in those cases which are negative for JEV-specific IgM antibodies in both serum and CSF samples. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/19249068/Evaluation_of_reverse_transcriptase_PCR_as_a_diagnostic_tool_to_confirm_Japanese_encephalitis_virus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035-9203(09)00022-4 DB - PRIME DP - Unbound Medicine ER -