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Identification and characterization of zinc-starvation-induced ZIP transporters from barley roots.
Plant Physiol Biochem. 2009 May; 47(5):377-83.PP

Abstract

Zinc (Zn) is an essential element for plants but limited information is currently available on the molecular basis for Zn(2+) transport in crop species. To expand the knowledge on Zn(2+) transport in barley (Hordeum vulgare L.), a cDNA library prepared from barley roots was expressed in the yeast (Saccharomyces cerevisiae) mutant strain Deltazrt1/Deltazrt2, defective in Zn(2+) uptake. This strategy resulted in isolation and identification of three new Zn(2+) transporters from barley. All of the predicted proteins have a high similarity to the ZIP protein family, and are designated HvZIP3, HvZIP5 and HvZIP8, respectively. Complementation studies in Deltazrt1/Deltazrt2 showed restored growth of the yeast cells transformed with the different HvZIPs, although with different efficiency. Transformation into Fe(2+) and Mn(2+) uptake defective yeast mutants showed that the HvZIPs were unable to restore the growth on Fe(2+) and Mn(2+) limited media, respectively, indicating a specific role in Zn(2+) transport. In intact barley roots, HvZIP8 was constitutively expressed whereas HvZIP3 and HvZIP5 were mainly expressed in -Zn plants. These results suggest that HvZIP3, HvZIP5 and HvZIP8 are Zn(2+) transporters involved in Zn(2+) homeostasis in barley roots. The new transporters may facilitate breeding of barley genotypes with improved Zn efficiency and Zn content.

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Authors+Show Affiliations

Pedas P
Department of Agriculture and Ecology, University of Copenhagen, Denmark. pp@life.ku.dk
Schjoerring JK
No affiliation info available
Husted S
No affiliation info available

MeSH

Amino Acid SequenceCarrier ProteinsCation Transport ProteinsGene Expression Regulation, PlantGenetic Complementation TestHordeumIon TransportIronManganeseMolecular Sequence DataMutationPhylogenyPlant ProteinsPlant RootsReverse Transcriptase Polymerase Chain ReactionSaccharomyces cerevisiaeSequence Homology, Amino AcidZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19249224

Citation

Pedas, Pai, et al. "Identification and Characterization of Zinc-starvation-induced ZIP Transporters From Barley Roots." Plant Physiology and Biochemistry : PPB, vol. 47, no. 5, 2009, pp. 377-83.
Pedas P, Schjoerring JK, Husted S. Identification and characterization of zinc-starvation-induced ZIP transporters from barley roots. Plant Physiol Biochem. 2009;47(5):377-83.
Pedas, P., Schjoerring, J. K., & Husted, S. (2009). Identification and characterization of zinc-starvation-induced ZIP transporters from barley roots. Plant Physiology and Biochemistry : PPB, 47(5), 377-83. https://doi.org/10.1016/j.plaphy.2009.01.006
Pedas P, Schjoerring JK, Husted S. Identification and Characterization of Zinc-starvation-induced ZIP Transporters From Barley Roots. Plant Physiol Biochem. 2009;47(5):377-83. PubMed PMID: 19249224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of zinc-starvation-induced ZIP transporters from barley roots. AU - Pedas,Pai, AU - Schjoerring,Jan K, AU - Husted,Søren, Y1 - 2009/02/07/ PY - 2008/09/16/received PY - 2009/01/30/revised PY - 2009/01/30/accepted PY - 2009/3/3/entrez PY - 2009/3/3/pubmed PY - 2009/10/22/medline SP - 377 EP - 83 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 47 IS - 5 N2 - Zinc (Zn) is an essential element for plants but limited information is currently available on the molecular basis for Zn(2+) transport in crop species. To expand the knowledge on Zn(2+) transport in barley (Hordeum vulgare L.), a cDNA library prepared from barley roots was expressed in the yeast (Saccharomyces cerevisiae) mutant strain Deltazrt1/Deltazrt2, defective in Zn(2+) uptake. This strategy resulted in isolation and identification of three new Zn(2+) transporters from barley. All of the predicted proteins have a high similarity to the ZIP protein family, and are designated HvZIP3, HvZIP5 and HvZIP8, respectively. Complementation studies in Deltazrt1/Deltazrt2 showed restored growth of the yeast cells transformed with the different HvZIPs, although with different efficiency. Transformation into Fe(2+) and Mn(2+) uptake defective yeast mutants showed that the HvZIPs were unable to restore the growth on Fe(2+) and Mn(2+) limited media, respectively, indicating a specific role in Zn(2+) transport. In intact barley roots, HvZIP8 was constitutively expressed whereas HvZIP3 and HvZIP5 were mainly expressed in -Zn plants. These results suggest that HvZIP3, HvZIP5 and HvZIP8 are Zn(2+) transporters involved in Zn(2+) homeostasis in barley roots. The new transporters may facilitate breeding of barley genotypes with improved Zn efficiency and Zn content. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/19249224/Identification_and_characterization_of_zinc_starvation_induced_ZIP_transporters_from_barley_roots_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(09)00042-4 DB - PRIME DP - Unbound Medicine ER -