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Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries.
Int J Paediatr Dent. 2009 Mar; 19(2):141-7.IJ

Abstract

BACKGROUND

Streptococcus mutans and Streptococcus sobrinus are closely associated with the development of early childhood caries (ECC). Recently, quantitative real-time polymerase chain reaction (qRT-PCR) has been used for rapid and accurate quantification of these bacterial species.

AIM

This study aims to detect quantitatively the levels of S. mutans and S. sobrinus in plaque samples by qRT-PCR, and to assess their association with the prevalence of ECC in Korean preschool children.

DESIGN

One hundred and five children (71 months old or younger) were examined and classified into three groups (caries-free, ECC, severe ECC). Dental plaque samples were collected and qRT-PCR was conducted using oligonucleotide primers specific for glucosyltransferase gene (S. mutans-gtfB, S. sobrinus-gtfU) and universal primer. Pearson's correlation test was conducted to evaluate the relationship between the dmfs (decayed, missing, or filled surfaces primary teeth) scores and the microbiological findings.

RESULTS

There was a significant difference between the levels of S. mutans and S. sobrinus in the plaque samples of the three groups (P < 0.05). The proportion of S. sobrinus to S. mutans showed strong correlation to the dmfs scores (r = 0.748, P < 0.05).

CONCLUSIONS

The qRT-PCR results of this study showed that children with ECC had higher level of S. mutans and S. sobrinus in their dental plaque samples. The children with higher ratio of S. sobrinus to S. mutans in their dental plaque showed higher incidence of ECC.

Authors+Show Affiliations

Department of Pediatric Dentistry, School of Dentistry, Seoul National University, Seoul, South Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19250396

Citation

Choi, Eun-Jung, et al. "Quantitative Real-time Polymerase Chain Reaction for Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque Samples and Its Association With Early Childhood Caries." International Journal of Paediatric Dentistry, vol. 19, no. 2, 2009, pp. 141-7.
Choi EJ, Lee SH, Kim YJ. Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries. Int J Paediatr Dent. 2009;19(2):141-7.
Choi, E. J., Lee, S. H., & Kim, Y. J. (2009). Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries. International Journal of Paediatric Dentistry, 19(2), 141-7. https://doi.org/10.1111/j.1365-263X.2008.00942.x
Choi EJ, Lee SH, Kim YJ. Quantitative Real-time Polymerase Chain Reaction for Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque Samples and Its Association With Early Childhood Caries. Int J Paediatr Dent. 2009;19(2):141-7. PubMed PMID: 19250396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries. AU - Choi,Eun-Jung, AU - Lee,Sung-Hoon, AU - Kim,Young-Jae, PY - 2009/3/3/entrez PY - 2009/3/3/pubmed PY - 2009/7/1/medline SP - 141 EP - 7 JF - International journal of paediatric dentistry JO - Int J Paediatr Dent VL - 19 IS - 2 N2 - BACKGROUND: Streptococcus mutans and Streptococcus sobrinus are closely associated with the development of early childhood caries (ECC). Recently, quantitative real-time polymerase chain reaction (qRT-PCR) has been used for rapid and accurate quantification of these bacterial species. AIM: This study aims to detect quantitatively the levels of S. mutans and S. sobrinus in plaque samples by qRT-PCR, and to assess their association with the prevalence of ECC in Korean preschool children. DESIGN: One hundred and five children (71 months old or younger) were examined and classified into three groups (caries-free, ECC, severe ECC). Dental plaque samples were collected and qRT-PCR was conducted using oligonucleotide primers specific for glucosyltransferase gene (S. mutans-gtfB, S. sobrinus-gtfU) and universal primer. Pearson's correlation test was conducted to evaluate the relationship between the dmfs (decayed, missing, or filled surfaces primary teeth) scores and the microbiological findings. RESULTS: There was a significant difference between the levels of S. mutans and S. sobrinus in the plaque samples of the three groups (P < 0.05). The proportion of S. sobrinus to S. mutans showed strong correlation to the dmfs scores (r = 0.748, P < 0.05). CONCLUSIONS: The qRT-PCR results of this study showed that children with ECC had higher level of S. mutans and S. sobrinus in their dental plaque samples. The children with higher ratio of S. sobrinus to S. mutans in their dental plaque showed higher incidence of ECC. SN - 1365-263X UR - https://www.unboundmedicine.com/medline/citation/19250396/Quantitative_real_time_polymerase_chain_reaction_for_Streptococcus_mutans_and_Streptococcus_sobrinus_in_dental_plaque_samples_and_its_association_with_early_childhood_caries_ DB - PRIME DP - Unbound Medicine ER -