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IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats.
Vet Parasitol. 2009 May 12; 161(3-4):356-9.VP

Abstract

The aims of this study were to analyze the systemic IgG responses against third-instar salivary gland (L3SG) antigens by ELISA in Oestrus ovis experimentally infected kids (EIK) and in naturally exposed adult goats (NEG). Firstly, kids (n=4 per group) were assigned to receive intranasally 0, 12, 24, 36, and 48 first-instars in experimental infections. Blood samples were taken from EIK at Days 0, 14, 42 and 67 post-infection. At necropsy (Day 67), larval number and developmental instars were recorded. In an epidemiological study, blood serum samples were collected from 448 grazing NEG (n=20 flocks) in Baja California Sur, Mexico. Results showed that larval establishment rate was similar in EIK groups. Systemic IgG response reached the threshold after Day 42, but humoral response was not statistically different among EIK groups receiving experimental infections. In NEG, all surveyed flocks (100%) showed specific systemic IgG antibodies to L3SG antigens and the overall goat oestrosis prevalence was 59.2%. In conclusion, larval L3SG antigens were effective in detection of specific systemic IgG antibodies against O. ovis infected kids and goats by ELISA.

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Authors+Show Affiliations

Angulo-Valadez CE
Centro de Investigaciones Biológicas del Noroeste, Mar Bermejo 195, Col. Playa de Santa Rita, La Paz, B.C.S. 23090, Mexico.
Cepeda-Palacios R
No affiliation info available
Ascencio F
No affiliation info available
Jacquiet P
No affiliation info available
Dorchies P
No affiliation info available
Ramírez-Orduña JM
No affiliation info available
López MA
No affiliation info available

MeSH

AnimalsDipteraFemaleGoat DiseasesGoatsImmunoglobulin GInsect ProteinsLarvaMaleNasal MucosaSalivary Glands

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19251372

Citation

Angulo-Valadez, C E., et al. "IgG Antibody Response Against Salivary Gland Antigens From Oestrus Ovis L. Larvae (Diptera: Oestridae) in Experimentally and Naturally Infected Goats." Veterinary Parasitology, vol. 161, no. 3-4, 2009, pp. 356-9.
Angulo-Valadez CE, Cepeda-Palacios R, Ascencio F, et al. IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats. Vet Parasitol. 2009;161(3-4):356-9.
Angulo-Valadez, C. E., Cepeda-Palacios, R., Ascencio, F., Jacquiet, P., Dorchies, P., Ramírez-Orduña, J. M., & López, M. A. (2009). IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats. Veterinary Parasitology, 161(3-4), 356-9. https://doi.org/10.1016/j.vetpar.2009.01.027
Angulo-Valadez CE, et al. IgG Antibody Response Against Salivary Gland Antigens From Oestrus Ovis L. Larvae (Diptera: Oestridae) in Experimentally and Naturally Infected Goats. Vet Parasitol. 2009 May 12;161(3-4):356-9. PubMed PMID: 19251372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgG antibody response against salivary gland antigens from Oestrus ovis L. larvae (Diptera: Oestridae) in experimentally and naturally infected goats. AU - Angulo-Valadez,C E, AU - Cepeda-Palacios,R, AU - Ascencio,F, AU - Jacquiet,Ph, AU - Dorchies,Ph, AU - Ramírez-Orduña,J M, AU - López,M A, Y1 - 2009/02/06/ PY - 2008/07/08/received PY - 2009/01/21/revised PY - 2009/01/26/accepted PY - 2009/3/3/entrez PY - 2009/3/3/pubmed PY - 2009/7/3/medline SP - 356 EP - 9 JF - Veterinary parasitology JO - Vet Parasitol VL - 161 IS - 3-4 N2 - The aims of this study were to analyze the systemic IgG responses against third-instar salivary gland (L3SG) antigens by ELISA in Oestrus ovis experimentally infected kids (EIK) and in naturally exposed adult goats (NEG). Firstly, kids (n=4 per group) were assigned to receive intranasally 0, 12, 24, 36, and 48 first-instars in experimental infections. Blood samples were taken from EIK at Days 0, 14, 42 and 67 post-infection. At necropsy (Day 67), larval number and developmental instars were recorded. In an epidemiological study, blood serum samples were collected from 448 grazing NEG (n=20 flocks) in Baja California Sur, Mexico. Results showed that larval establishment rate was similar in EIK groups. Systemic IgG response reached the threshold after Day 42, but humoral response was not statistically different among EIK groups receiving experimental infections. In NEG, all surveyed flocks (100%) showed specific systemic IgG antibodies to L3SG antigens and the overall goat oestrosis prevalence was 59.2%. In conclusion, larval L3SG antigens were effective in detection of specific systemic IgG antibodies against O. ovis infected kids and goats by ELISA. SN - 0304-4017 UR - https://www.unboundmedicine.com/medline/citation/19251372/IgG_antibody_response_against_salivary_gland_antigens_from_Oestrus_ovis_L__larvae__Diptera:_Oestridae__in_experimentally_and_naturally_infected_goats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(09)00079-X DB - PRIME DP - Unbound Medicine ER -